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AB189254

Anti-alpha Sarcoglycan antibody [EPR14773]

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(16 Publications)

Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254) is a rabbit monoclonal antibody detecting alpha Sarcoglycan in Western Blot, IP, IHC-P. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

ADL, DAG2, SGCA, Alpha-sarcoglycan, Alpha-SG, 50 kDa dystrophin-associated glycoprotein, Adhalin, Dystroglycan-2, 50DAG

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)

Immunhistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling alpha Sarcoglycan with ab189254 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)
  • IP

Supplier Data

Immunoprecipitation - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)

Immunoprecipitation analysis of human skeletal muscle tissue lysate labeling alpha Sarcoglycan using ab189254 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2 : PBS instead of human skeletal muscle tissue lysate.

All lanes:

Immunoprecipitation - Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254)

Predicted band size: 43 kDa

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Western blot - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)
  • WB

Supplier Data

Western blot - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)

All lanes:

Western blot - Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254) at 1/1000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 50 kDa

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Western blot - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)
  • WB

Supplier Data

Western blot - Anti-alpha Sarcoglycan antibody [EPR14773] (AB189254)

All lanes:

Western blot - Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254) at 1/10000 dilution

All lanes:

Human skeletal muscle lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 50 kDa

false

  • Carrier free

    Anti-alpha Sarcoglycan antibody [EPR14773] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-alpha Sarcoglycan antibody [EPR14773]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-alpha Sarcoglycan antibody [EPR14773]

  • HRP

    HRP Anti-alpha Sarcoglycan antibody [EPR14773]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14773

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-alpha Sarcoglycan antibody [EPR14773] (ab189254) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of alpha Sarcoglycan?
Anti-alpha Sarcoglycan [EPR14773] (ab189254) specifically detects a band for alpha Sarcoglycan (UniProt: Q16586) at a molecular weight of 43kDa.

Trusted by the scientific community
Anti-alpha Sarcoglycan [EPR14773] (ab189254) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR14773] also available for your convenience: ab189254, Carrier free - ab240307, PE - ab309435, Alexa Fluor® 647 - ab320777, Alexa Fluor® 488 - ab321851, HRP - ab323780

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Sarcoglycan also known as Adhalin or 50DAG is a protein with a molecular mass of approximately 50 kDa. It is an important component in skeletal and cardiac muscle tissue. This protein is part of the dystrophin-glycoprotein complex (DGC) which connects the cytoskeleton of a muscle fiber to the extracellular matrix. Alpha Sarcoglycan is primarily expressed in striated muscle cells including those of skeletal and cardiac muscles but also at low levels in other tissues.
Biological function summary

Alpha Sarcoglycan contributes to the integrity and function of muscle fibers by stabilizing the DGC which includes sarcoglycans and other associated proteins. This stabilization provides mechanical support during muscle contraction and stretching. Alpha Sarcoglycan's role in the complex ensures proper signal transduction and protects muscle fibers from injury that may occur through mechanical stress. The protein's interaction with other sarcoglycans such as beta gamma and delta facilitates these structural and signaling functions.

Pathways

Alpha Sarcoglycan is involved in muscle development and maintenance pathways. It plays a role in the mechanotransduction signaling pathway which helps cells convert mechanical stimuli into chemical activity. The DGC including alpha Sarcoglycan works closely with dystrophin an important protein in the process. This relationship is essential for maintaining the structural integrity of muscle fibers and impacts pathways regulating muscle repair and regeneration.

Mutations in the alpha Sarcoglycan gene are linked to limb-girdle muscular dystrophy type 2D (LGMD2D). This condition is characterized by progressive muscle weakness particularly affecting the shoulders and hips. The disruption of alpha Sarcoglycan affects its interaction with other sarcoglycan proteins leading to the disassembly of the DGC and causing increased muscle cell damage. Understanding the role of alpha Sarcoglycan can also provide insights into other disorders related to defects in the DGC.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
See full target information SGCA
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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40183632

2025

AOC 1044 induces exon 44 skipping and restores dystrophin protein in preclinical models of Duchenne muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Usue Etxaniz,Isaac Marks,Tyler Albin,Matthew Diaz,Raghav Bhardwaj,Aaron Anderson,Olecya Tyaglo,Tiffany Hoang,Maria Azzurra Missinato,Kristoffer Svensson,Ben Badillo,Philip R Kovach,Laura Leung,Michael Cochran,Hae Won Kwon,Md Nur Ahad Shah,Rika Maruyama,Toshifumi Yokota,Venkata R Doppalapudi,Beatrice Darimont,Husam S Younis,W Michael Flanagan,Arthur A Levin,Hanhua Huang,Georgios Karamanlidis

Materials today. Bio 28:101180 PubMed39221216

2024

A photothermal responsive system accelerating nitric oxide release to enhance bone repair by promoting osteogenesis and angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yannan Cheng,Yuanfang Huo,Yongle Yu,Ping Duan,Xianzhen Dong,Zirui Yu,Qiang Cheng,Honglian Dai,Zhenyu Pan

Experimental and therapeutic medicine 27:97 PubMed38356676

2024

Identification and functional characterization of a novel heterozygous splice‑site mutation in the calpain 3 gene causes rare autosomal dominant limb‑girdle muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Mao,Jie Yang,Xiaodong Zhao,Xueling Jia,Xin Shi,Lihui Zhao,Santasree Banerjee,Lili Zhang,Xiaoling Ma

ACS medicinal chemistry letters 14:1049-1053 PubMed37583821

2023

Synthesis and Evaluation of Bithiazole Derivatives As Potential α-Sarcoglycan Correctors.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni Ribaudo,Marcello Carotti,Alberto Ongaro,Erika Oselladore,Martina Scano,Giuseppe Zagotto,Dorianna Sandonà,Alessandra Gianoncelli

Molecular therapy. Methods & clinical development 30:161-180 PubMed37457303

2023

CRISPR-Cas9 correction in the DMD mouse model is accompanied by upregulation of Dp71f protein.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana V Egorova,Anna V Polikarpova,Svetlana G Vassilieva,Marina A Dzhenkova,Irina M Savchenko,Oleg A Velyaev,Anna A Shmidt,Vladislav O Soldatov,Mikhail V Pokrovskii,Alexey V Deykin,Maryana V Bardina

International journal of molecular sciences 24: PubMed37298068

2023

In-Frame Deletion of Dystrophin Exons 8-50 Results in DMD Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana V Egorova,Ivan I Galkin,Oleg A Velyaev,Svetlana G Vassilieva,Irina M Savchenko,Vyacheslav A Loginov,Marina A Dzhenkova,Diana S Korshunova,Olga S Kozlova,Dmitry N Ivankov,Anna V Polikarpova

Molecular therapy. Methods & clinical development 28:284-299 PubMed36816759

2023

Systemic γ-sarcoglycan AAV gene transfer results in dose-dependent correction of muscle deficits in the LGMD 2C/R5 mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Young-Eun Seo,Stephen H Baine,Amber N Kempton,Oliver C Rogers,Sarah Lewis,Kaitlin Adegboye,Alex Haile,Danielle A Griffin,Ellyn L Peterson,Eric R Pozsgai,Rachael A Potter,Louise R Rodino-Klapac

Scientific reports 12:848 PubMed35039573

2022

Therapeutic potential of highly functional codon-optimized microutrophin for muscle-specific expression.

Applications

Unspecified application

Species

Unspecified reactive species

Anna V Starikova,Victoria V Skopenkova,Anna V Polikarpova,Denis A Reshetov,Svetlana G Vassilieva,Oleg A Velyaev,Anna A Shmidt,Irina M Savchenko,Vladislav O Soldatov,Tatiana V Egorova,Maryana V Bardina

Human molecular genetics 31:499-509 PubMed34505136

2021

CFTR corrector C17 is effective in muscular dystrophy, in vivo proof of concept in LGMDR3.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Scano,Alberto Benetollo,Leonardo Nogara,Michela Bondì,Francesco Dalla Barba,Michela Soardi,Sandra Furlan,Eylem Emek Akyurek,Paola Caccin,Marcello Carotti,Roberta Sacchetto,Bert Blaauw,Dorianna Sandonà

Communications biology 4:427 PubMed33782502

2021

MiR-199-3p enhances muscle regeneration and ameliorates aged muscle and muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Masashi Fukuoka,Hiromi Fujita,Kosumo Numao,Yasuko Nakamura,Hideo Shimizu,Masayuki Sekiguchi,Hirohiko Hohjoh
View all publications
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