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AB255739

Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free

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Mouse Recombinant Monoclonal alpha Sarcoglycan antibody. Carrier free. Suitable for IHC-Fr and reacts with Mouse, Rat, Human samples.

View Alternative Names

ADL, DAG2, SGCA, Alpha-sarcoglycan, Alpha-SG, 50 kDa dystrophin-associated glycoprotein, Adhalin, Dystroglycan-2, 50DAG

4 Images
Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (AB255739)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (AB255739)

This data was developed using ab234589, the same clone but in a different formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse heart (fresh) tissue labeling alpha Sarcoglycan with ab234589 at a 1/100 dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 μg/ml) (Green). Membranous staining on mouse heart is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (AB255739)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (AB255739)

This data was developed using ab234589, the same clone but in a different formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen human heart tissue labeling alpha Sarcoglycan. Membranous staining on human heart. The section was blocked using Bloxall™ for 20 mins at room temperature, then incubated with ab234589, 1 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. It was then counterstained with haematoxylin and mounted with DPX. The nuclear counterstain was DAPI (Blue).

Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (AB255739)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (AB255739)

This data was developed using ab234589, the same clone but in a different formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse spleen (fresh) tissue labeling alpha Sarcoglycan with ab234589 at a 1/100 dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution (Green). Negative control : No staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (AB255739)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-alpha Sarcoglycan antibody [IVD3-A9] - BSA and Azide free (AB255739)

This data was developed using ab234589, the same clone but in a different formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling alpha Sarcoglycan. Membranous staining on human heart. The section was blocked using Bloxall™ for 20 mins at room temperature, then incubated with ab234589, 1 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. It was then counterstained with haematoxylin and mounted with DPX. The nuclear counterstain was DAPI (Blue).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

IVD3-A9

Isotype

IgG1

Carrier free

Yes

Reacts with

Mouse, Human, Rat

Applications

IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab255739 is the carrier free version of ab234589.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Sarcoglycan also known as Adhalin or 50DAG is a protein with a molecular mass of approximately 50 kDa. It is an important component in skeletal and cardiac muscle tissue. This protein is part of the dystrophin-glycoprotein complex (DGC) which connects the cytoskeleton of a muscle fiber to the extracellular matrix. Alpha Sarcoglycan is primarily expressed in striated muscle cells including those of skeletal and cardiac muscles but also at low levels in other tissues.
Biological function summary

Alpha Sarcoglycan contributes to the integrity and function of muscle fibers by stabilizing the DGC which includes sarcoglycans and other associated proteins. This stabilization provides mechanical support during muscle contraction and stretching. Alpha Sarcoglycan's role in the complex ensures proper signal transduction and protects muscle fibers from injury that may occur through mechanical stress. The protein's interaction with other sarcoglycans such as beta gamma and delta facilitates these structural and signaling functions.

Pathways

Alpha Sarcoglycan is involved in muscle development and maintenance pathways. It plays a role in the mechanotransduction signaling pathway which helps cells convert mechanical stimuli into chemical activity. The DGC including alpha Sarcoglycan works closely with dystrophin an important protein in the process. This relationship is essential for maintaining the structural integrity of muscle fibers and impacts pathways regulating muscle repair and regeneration.

Mutations in the alpha Sarcoglycan gene are linked to limb-girdle muscular dystrophy type 2D (LGMD2D). This condition is characterized by progressive muscle weakness particularly affecting the shoulders and hips. The disruption of alpha Sarcoglycan affects its interaction with other sarcoglycan proteins leading to the disassembly of the DGC and causing increased muscle cell damage. Understanding the role of alpha Sarcoglycan can also provide insights into other disorders related to defects in the DGC.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
See full target information SGCA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com