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Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for ICC, WB and reacts with Human, Rat samples. Cited in 50 publications. Immunogen corresponding to Tissue preparation containing ACTA1 protein.


Images

Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (AB28052), expandable thumbnail
  • Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (AB28052), expandable thumbnail
  • Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (AB28052), expandable thumbnail
  • Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (AB28052), expandable thumbnail

Publications

Key facts

Isotype
IgM
Host species
Mouse
Storage buffer

pH: 7.2
Preservative: 0.097% Sodium azide
Constituents: 0.79% Tris HCl

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Tissue preparation containing ACTA1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P68135

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWB
Human
Tested
Expected
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Additional Targets

Acta1

Alternative names

Recommended products

Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for ICC, WB and reacts with Human, Rat samples. Cited in 50 publications. Immunogen corresponding to Tissue preparation containing ACTA1 protein.

Key facts

Isotype
IgM
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Tissue preparation containing ACTA1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P68135
Clone number
Alpha Sr-1
Purity
Tissue culture supernatant
Specificity

In Western blotting of pure rabbit striated muscle actin or of total extracts from different tissues, e.g. rat striated muscle, rat myocardium, rat aortic media, human fibroblasts and pure chicken gizzard actin, ab28052 labels alpha-skeletal (alpha-sarcomeric) and alpha-cardiac muscle actins. No labelling is observed with actin from fibroblasts (alpha-smooth muscle and beta-cytoplasmic actin), aorta (alpha-smooth muscle actin), or chicken gizzard (gamma-smooth muscle actin).

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha Skeletal Muscle Actin also known as Actin Alpha 1 Actin Alpha and Actin Protein is a highly conserved protein important for muscle function. It has a molecular mass of approximately 42 kDa. This protein is expressed in skeletal muscle tissues and is an essential component of muscle cell's cytoskeleton. The actin family to which Alpha Skeletal Muscle Actin belongs is integral for maintaining cell shape integrity and movement. This isoform of actin is especially significant in forming thin filaments in muscle fibers which are essential for muscle contraction.

Biological function summary

Alpha Skeletal Muscle Actin plays an essential role in the contractile apparatus of muscle cells. It exists as part of the actin filament complex interacting closely with myosin motor proteins. This interaction powers the sliding mechanism that enables muscle contraction and relaxation. Alpha-actin is responsible for mechanical stiffness and tension within muscle tissues enabling muscle fibers to withstand physical stress. The protein's structural role in muscle cells makes it indispensable for proper muscle function and muscle fiber stability.

Pathways

Alpha Skeletal Muscle Actin is deeply involved in the cytoskeletal organization and muscle contraction pathways. It is pivotal in the actin-myosin interaction pathway where it binds to troponin and tropomyosin which regulate muscle contraction cycles. Within these pathways the protein ensures coordinated muscle contractions alongside other proteins like myosin heavy chain and tropomyosin. These interactions coordinate muscle contraction maintaining muscle tone and health.

Associated diseases and disorders

Alpha Skeletal Muscle Actin mutations have a direct link to various myopathies including actin myopathy and nemaline myopathy. These conditions often result in muscle weakness and occasionally muscle degeneration. The protein's malfunction may also connect to cardiomyopathies due to its important role in muscle function. Research suggests that protein interactions with dystrophin another muscle-associated protein might further elucidate its role in these muscle disorders. Actin dysfunction in these conditions highlights the necessity for healthy actin filaments for skeletal muscle integrity and function.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (ab28052), expandable thumbnail

    Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (ab28052)

    Alpha Skeletal Muscle Actin Immunocytochemistry staining using mouse Anti-Alpha Skeletal Muscle Actin antibody

    Immunocytochemistry analysis of HeLa cells labeling Alpha Skeletal Muscle Actin with ab28052 at 1/1000. Cells were fixed and permeabilized with methanol followed by aceton. The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

  • Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (ab28052), expandable thumbnail

    Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (ab28052)

    Alpha Skeletal Muscle Actin Western blot staining of Whole extract of Rat heart using mouse Anti-Alpha Skeletal Muscle Actin antibody

    chemiluminescent substrate.

    Lane 1: Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (ab28052) at 1/500 dilution

    Lane 2: Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (ab28052) at 1/1000 dilution

    Lane 3: Mouse Monoclonal Anti-Actin (α-Sarcomeric) antibody at 1/500 dilution

    All lanes: Whole extract of Rat heart

    Secondary

    All lanes: Goat Anti-Mouse IgG-Peroxidase

    Predicted band size: 42 kDa

  • Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (ab28052), expandable thumbnail
    This image is courtesy of a customer review submitted by Richard Patten.

    Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (ab28052)

    Alpha Skeletal Muscle Actin Immunocytochemistry staining using mouse Anti-Alpha Skeletal Muscle Actin antibody

    ab28052 at dilution 1/200 staining alpha sarcomeric actin from rat neonatal cardiac myocytes by immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% NP40 and incubated with the antibody for 45 minutes. A Cy3® donkey anti-mouse antibody was used as the secondary antibody.

    This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.

  • Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (ab28052), expandable thumbnail
    This image is courtesy of a customer review submitted by Richard Patten.

    Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (ab28052)

    Alpha Skeletal Muscle Actin Western blot staining of 40 µg rat neonatal cardiac myocytes (whole cell lysate) using mouse Anti-Alpha Skeletal Muscle Actin antibody

    This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.

    All lanes: Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (ab28052) at 1/200 dilution

    All lanes: 40 µg rat neonatal cardiac myocytes (whole cell lysate)

    Secondary

    All lanes: HRP polymer conjugated donkey anti-mouse IgG at 1/1000

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa

    Observed band size: 42 kDa

    Exposure time: 1s

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Product protocols

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