AB28052

Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control

Name: Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (ab28052) | Abcam
Brand: Abcam Limited
SKU: AB28052
Price: 645 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(55 Publications)

Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for ICC, WB and reacts with Human, Rat samples. Cited in 55 publications. Immunogen corresponding to Tissue preparation containing ACTA1 protein.

View Alternative Names

ACTA, ACTA1, Alpha-actin-1

4 Images

Supplier Data

Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (AB28052)

Immunocytochemistry analysis of HeLa cells labeling Alpha Skeletal Muscle Actin with ab28052 at 1/1000. Cells were fixed and permeabilized with methanol followed by aceton. The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

AbReview10776****

Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (AB28052)

ab28052 at dilution 1/200 staining alpha sarcomeric actin from rat neonatal cardiac myocytes by immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% NP40 and incubated with the antibody for 45 minutes. A Cy3® donkey anti-mouse antibody was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.

This image is courtesy of an Abreview submitted by Richard Patten.

Supplier Data

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (AB28052)

chemiluminescent substrate.

Lane 1:

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (ab28052) at 1/500 dilution

Lane 2:

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (ab28052) at 1/1000 dilution

Lane 3:

Mouse Monoclonal Anti-Actin (α-Sarcomeric) antibody at 1/500 dilution

All lanes:

Whole extract of Rat heart

Secondary

All lanes:

Goat Anti-Mouse IgG-Peroxidase

Predicted band size: 42 kDa

false

AbReview10777****

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (AB28052)

This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.

All lanes:

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (ab28052) at 1/200 dilution

All lanes:

40 µg rat neonatal cardiac myocytes (whole cell lysate)

Secondary

All lanes:

HRP polymer conjugated donkey anti-mouse IgG at 1/1000

Predicted band size: 42 kDa

Observed band size: 42 kDa

true

Exposure time: 1s

This image is courtesy of an Abreview submitted by Richard Patten.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Alpha Sr-1

Isotype

IgM

Carrier free

Reacts with

Rat, Human

Applications

ICC, WB

applications

Immunogen

Tissue preparation containing ACTA1 protein. The exact immunogen used to generate this antibody is proprietary information.

P68135

Specificity

In Western blotting of pure rabbit striated muscle actin or of total extracts from different tissues, e.g. rat striated muscle, rat myocardium, rat aortic media, human fibroblasts and pure chicken gizzard actin, ab28052 labels alpha-skeletal (alpha-sarcomeric) and alpha-cardiac muscle actins. No labelling is observed with actin from fibroblasts (alpha-smooth muscle and beta-cytoplasmic actin), aorta (alpha-smooth muscle actin), or chicken gizzard (gamma-smooth muscle actin).

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 Preservative: 0.097% Sodium azide Constituents: 0.79% Tris HCl

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Skeletal Muscle Actin also known as Actin Alpha 1 Actin Alpha and Actin Protein is a highly conserved protein important for muscle function. It has a molecular mass of approximately 42 kDa. This protein is expressed in skeletal muscle tissues and is an essential component of muscle cell's cytoskeleton. The actin family to which Alpha Skeletal Muscle Actin belongs is integral for maintaining cell shape integrity and movement. This isoform of actin is especially significant in forming thin filaments in muscle fibers which are essential for muscle contraction.

Biological function summary

Alpha Skeletal Muscle Actin plays an essential role in the contractile apparatus of muscle cells. It exists as part of the actin filament complex interacting closely with myosin motor proteins. This interaction powers the sliding mechanism that enables muscle contraction and relaxation. Alpha-actin is responsible for mechanical stiffness and tension within muscle tissues enabling muscle fibers to withstand physical stress. The protein's structural role in muscle cells makes it indispensable for proper muscle function and muscle fiber stability.

Pathways

Alpha Skeletal Muscle Actin is deeply involved in the cytoskeletal organization and muscle contraction pathways. It is pivotal in the actin-myosin interaction pathway where it binds to troponin and tropomyosin which regulate muscle contraction cycles. Within these pathways the protein ensures coordinated muscle contractions alongside other proteins like myosin heavy chain and tropomyosin. These interactions coordinate muscle contraction maintaining muscle tone and health.

Alpha Skeletal Muscle Actin mutations have a direct link to various myopathies including actin myopathy and nemaline myopathy. These conditions often result in muscle weakness and occasionally muscle degeneration. The protein's malfunction may also connect to cardiomyopathies due to its important role in muscle function. Research suggests that protein interactions with dystrophin another muscle-associated protein might further elucidate its role in these muscle disorders. Actin dysfunction in these conditions highlights the necessity for healthy actin filaments for skeletal muscle integrity and function.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA1

style

left-column

Publications (55)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 15:292 PubMed39256792

2024

Targeting NPM1 inhibits proliferation and promotes apoptosis of hepatic progenitor cells via suppression of mTOR signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Wang,Min Wang,Lin Liu,Hongyi Li,Helin Liu,Jiangbo Ren,Tianhui Liu,Min Cong,Zhijun Zhu,Xinyan Zhao,Liying Sun,Jidong Jia

Aging and disease 14:2215-2237 PubMed37199578

2023

IFNγ Transcribed by IRF1 in CD4+ Effector Memory T Cells Promotes Senescence-Associated Pulmonary Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyun Chen,Qiuyi Wang,Jie Li,Yuan Li,Ao Chen,Jiawen Zhou,Jingyu Zhao,Zhiyuan Mao,Zihao Zhou,Jin'ge Zhang,Yue Wang,Rong Wang,Qing Li,Yongjie Zhang,Runqiu Jiang,Dengshun Miao,Jianliang Jin

Clinical epigenetics 15:167 PubMed37858275

2023

Pharmacological EZH2 inhibition combined with retinoic acid treatment promotes differentiation and apoptosis in rhabdomyosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eleanor O'Brien,Carmen Tse,Ian Tracy,Ian Reddin,Joanna Selfe,Jane Gibson,William Tapper,Reuben J Pengelly,Jinhui Gao,Ewa Aladowicz,Gemma Petts,Khin Thway,Sergey Popov,Anna Kelsey,Timothy J Underwood,Janet Shipley,Zoë S Walters

Reviews in cardiovascular medicine 23:408 PubMed39076662

2022

Noninvasive Evaluation of Angiogenesis and Therapeutic Response after Hindlimb Ischemia with an Integrin-Targeted Tracer by PET.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongchan Sun,Weibin He,Shuang Xia,Guang Tong,Lin Zeng,Ling Xue,Junqing Yang,Ning Tan,Pengcheng He

Acta physiologica (Oxford, England) 236:e13869 PubMed36002394

2022

Citrullination is linked to reduced Ca sensitivity in hearts of a murine model of rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Gianluigi Pironti,Stefano Gastaldello,Dilson E Rassier,Johanna T Lanner,Mattias Carlström,Lars H Lund,Håkan Westerblad,Takashi Yamada,Daniel C Andersson

Aging cell 21:e13680 PubMed35906886

2022

Sirt1 overexpression improves senescence-associated pulmonary fibrosis induced by vitamin D deficiency through downregulating IL-11 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawen Zhou,Haiyun Chen,Qiuyi Wang,Sihan Chen,Rong Wang,Ziyang Wang,Cuicui Yang,Ao Chen,Jingyu Zhao,Zihao Zhou,Zhiyuan Mao,Guoping Zuo,Dengshun Miao,Jianliang Jin

Aging 14:4036-4049 PubMed35522573

2022

Pentraxin 3 depletion (PTX3 KD) inhibited myocardial fibrosis in heart failure after myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Yufang Xu,Yiting Hu,Yanping Geng,Na Zhao,Caiyun Jia,Haojing Song,Wanjun Bai,Caihui Guo,Lili Wang,Yanhui Ni,Xiaoyong Qi

Stem cells (Dayton, Ohio) 40:133-148 PubMed35257186

2022

Regulation of Myogenesis by a Na/K-ATPase α1 Caveolin-Binding Motif.

Applications

Unspecified application

Species

Unspecified reactive species

Minqi Huang,Xiaoliang Wang,Moumita Banerjee,Shreya T Mukherji,Laura C Kutz,Aijie Zhao,Michael Sepanski,Chen-Ming Fan,Guo-Zhang Zhu,Jiang Tian,Da-Zhi Wang,Hua Zhu,Zi-Jian Xie,Sandrine V Pierre,Liquan Cai

Biology 10: PubMed34827089

2021

PPARα, δ and FOXO1 Gene Silencing Overturns Palmitate-Induced Inhibition of Pyruvate Oxidation Differentially in C2C12 Myotubes.

Applications

Unspecified application

Species

Unspecified reactive species

Hung-Che Chien,Despina Constantin,Paul L Greenhaff,Dumitru Constantin-Teodosiu

Journal of cellular and molecular medicine 25:9767-9783 PubMed34547172

2021

LncRNA TUG1 attenuates ischaemia-reperfusion-induced apoptosis of renal tubular epithelial cells by sponging miR-144-3p via targeting Nrf2.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Zhao,Wu Chen,Wei Li,Weimin Yu,Siqi Li,Ting Rao,Yuan Ruan,Xiangjun Zhou,Cong Liu,Yucheng Qi,Fan Cheng

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control

Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (AB28052)

Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (AB28052)

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (AB28052)

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] - Loading Control (AB28052)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (55)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Reagents, Controls & Accessories

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise