Anti-Alpha Skeletal Muscle Actin antibody - Cytoskeleton Marker

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody - Cytoskeleton Marker (AB235928)

Paraffin-embedded human skeletal muscle tissue stained for Alpha Skeletal Muscle Actin using ab235928 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then, primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Alpha Skeletal Muscle Actin antibody - Cytoskeleton Marker (AB235928)

4% formaldehyde-fixed, 0.2% Triton X-100-permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Alpha Skeletal Muscle Actin using ab235928 at 1/133 dilution in ICC/IF. Cells were blocked in 10 % normal goat serum and incubated with primary antibody at 4°C overnight. Secondary antibody was Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (H+L).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody - Cytoskeleton Marker (AB235928)

Paraffin-embedded human cervical cancer tissue stained for Alpha Skeletal Muscle Actin using ab235928 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then, primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.