AB250775

Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free

Name: Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free (ab250775) | Abcam
Brand: Abcam Limited
SKU: AB250775
Price: 905 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(1 Publication)

Rabbit Recombinant Monoclonal alpha skeletal muscle Actin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

ACTA, ACTA1, Alpha-actin-1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free (AB250775)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free (AB250775)

This data was developed using ab184705, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Alpha Skeletal Muscle Actin with ab184705 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Weak cytoplasm staining in rat skeletal muscle is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free (AB250775)

This data was developed using ab184705, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Alpha Skeletal Muscle Actin with ab184705 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Weak cytoplasm staining in mouse skeletal muscle is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free (AB250775)

This data was developed using ab184705, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-skeletal-muscle-actin-antibody-epr18430-loading-control-ab184705'>ab184705</a>) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate at 10 µg

Lane 2:

Mouse skeletal muscle lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 5s

Supplier Data

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free (AB250775)

This data was developed using ab184705, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Mouse spleen tissue lysate at 10 µg

Lane 5:

Rat brain tissue lysate at 10 µg

Lane 6:

Rat heart tissue lysate at 10 µg

Lane 7:

Rat kidney tissue lysate at 10 µg

Lane 8:

Rat spleen tissue lysate at 10 µg

Lane 9:

Human brain tissue lysate at 10 µg

Lane 10:

Human heart tissue lysate at 10 µg

Lane 11:

Human kidney tissue lysate at 10 µg

Lane 12:

Human spleen tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free (AB250775)

This data was developed using ab184705, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Human bladder lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free (AB250775)

This data was developed using ab184705, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Rat skeletal muscle lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 1s

Unconjugated

Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18430

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

ab250775 is the carrier-free version of ab184705.

IHC is recommended for rat and mouse only.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Skeletal Muscle Actin also known as Actin Alpha 1 Actin Alpha and Actin Protein is a highly conserved protein important for muscle function. It has a molecular mass of approximately 42 kDa. This protein is expressed in skeletal muscle tissues and is an essential component of muscle cell's cytoskeleton. The actin family to which Alpha Skeletal Muscle Actin belongs is integral for maintaining cell shape integrity and movement. This isoform of actin is especially significant in forming thin filaments in muscle fibers which are essential for muscle contraction.

Biological function summary

Alpha Skeletal Muscle Actin plays an essential role in the contractile apparatus of muscle cells. It exists as part of the actin filament complex interacting closely with myosin motor proteins. This interaction powers the sliding mechanism that enables muscle contraction and relaxation. Alpha-actin is responsible for mechanical stiffness and tension within muscle tissues enabling muscle fibers to withstand physical stress. The protein's structural role in muscle cells makes it indispensable for proper muscle function and muscle fiber stability.

Pathways

Alpha Skeletal Muscle Actin is deeply involved in the cytoskeletal organization and muscle contraction pathways. It is pivotal in the actin-myosin interaction pathway where it binds to troponin and tropomyosin which regulate muscle contraction cycles. Within these pathways the protein ensures coordinated muscle contractions alongside other proteins like myosin heavy chain and tropomyosin. These interactions coordinate muscle contraction maintaining muscle tone and health.

Alpha Skeletal Muscle Actin mutations have a direct link to various myopathies including actin myopathy and nemaline myopathy. These conditions often result in muscle weakness and occasionally muscle degeneration. The protein's malfunction may also connect to cardiomyopathies due to its important role in muscle function. Research suggests that protein interactions with dystrophin another muscle-associated protein might further elucidate its role in these muscle disorders. Actin dysfunction in these conditions highlights the necessity for healthy actin filaments for skeletal muscle integrity and function.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 16:4716-4727 PubMed39678812

2024

Enhancing the radiosensitivity of colorectal cancer cells by reducing spermine synthase through promoting autophagy and DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Bin Guo,Yue-Ming Wu,Zhi-Zhao Lin

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free (AB250775)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free (AB250775)

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free (AB250775)

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free (AB250775)

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free (AB250775)

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free (AB250775)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Reagents, Controls & Accessories

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise