AB184705

Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control

RabMAb
Recombinant
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(11 Publications)

Rabbit Recombinant Monoclonal alpha skeletal muscle Actin antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 11 publications.

View Alternative Names

ACTA, ACTA1, Alpha-actin-1

6 Images

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (AB184705)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Alpha Skeletal Muscle Actin with ab184705 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Weak cytoplasm staining in rat skeletal muscle is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (AB184705)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Alpha Skeletal Muscle Actin with ab184705 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Weak cytoplasm staining in mouse skeletal muscle is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (AB184705)

All lanes:

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (ab184705) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate at 10 µg

Lane 2:

Mouse skeletal muscle lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 5s

Supplier Data

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (AB184705)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (ab184705) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Mouse spleen tissue lysate at 10 µg

Lane 5:

Rat brain tissue lysate at 10 µg

Lane 6:

Rat heart tissue lysate at 10 µg

Lane 7:

Rat kidney tissue lysate at 10 µg

Lane 8:

Rat spleen tissue lysate at 10 µg

Lane 9:

Human brain tissue lysate at 10 µg

Lane 10:

Human heart tissue lysate at 10 µg

Lane 11:

Human kidney tissue lysate at 10 µg

Lane 12:

Human spleen tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (AB184705)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (ab184705) at 1/1000 dilution

All lanes:

Human bladder lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (AB184705)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (ab184705) at 1/1000 dilution

All lanes:

Rat skeletal muscle lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 1s

Carrier free

Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18430

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

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Product details

IHC is recommended for rat and mouse only.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Skeletal Muscle Actin also known as Actin Alpha 1 Actin Alpha and Actin Protein is a highly conserved protein important for muscle function. It has a molecular mass of approximately 42 kDa. This protein is expressed in skeletal muscle tissues and is an essential component of muscle cell's cytoskeleton. The actin family to which Alpha Skeletal Muscle Actin belongs is integral for maintaining cell shape integrity and movement. This isoform of actin is especially significant in forming thin filaments in muscle fibers which are essential for muscle contraction.

Biological function summary

Alpha Skeletal Muscle Actin plays an essential role in the contractile apparatus of muscle cells. It exists as part of the actin filament complex interacting closely with myosin motor proteins. This interaction powers the sliding mechanism that enables muscle contraction and relaxation. Alpha-actin is responsible for mechanical stiffness and tension within muscle tissues enabling muscle fibers to withstand physical stress. The protein's structural role in muscle cells makes it indispensable for proper muscle function and muscle fiber stability.

Pathways

Alpha Skeletal Muscle Actin is deeply involved in the cytoskeletal organization and muscle contraction pathways. It is pivotal in the actin-myosin interaction pathway where it binds to troponin and tropomyosin which regulate muscle contraction cycles. Within these pathways the protein ensures coordinated muscle contractions alongside other proteins like myosin heavy chain and tropomyosin. These interactions coordinate muscle contraction maintaining muscle tone and health.

Alpha Skeletal Muscle Actin mutations have a direct link to various myopathies including actin myopathy and nemaline myopathy. These conditions often result in muscle weakness and occasionally muscle degeneration. The protein's malfunction may also connect to cardiomyopathies due to its important role in muscle function. Research suggests that protein interactions with dystrophin another muscle-associated protein might further elucidate its role in these muscle disorders. Actin dysfunction in these conditions highlights the necessity for healthy actin filaments for skeletal muscle integrity and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

BMC nephrology 26:526 PubMed41013408

2025

INHBA knockdown inhibits renal fibrosis in mice following ischemia-reperfusion injury by suppressing activation of the TGF-β/Smad signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yifei Wang,Qiao Tang,Qian Sun

The Korean journal of pain 38:244-257 PubMed40247654

2025

Correlation between knee pain and quadriceps condition in osteoarthritis rats treated with cog polydioxanone filaments.

Applications

Unspecified application

Species

Unspecified reactive species

Myeounghoon Cha,Guanghai Nan,Nari Kang,Sun Joon Bai,Ryo Ikeda,Bae Hwan Lee,Jun Ho Jang

Cell & bioscience 14:102 PubMed39160617

2024

SOX2 interacts with hnRNPK to modulate alternative splicing in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlan Huang,Yuxuan Liu,Mingyi Pu,Yuli Zhang,Qiang Cao,Senru Li,Yuanjie Wei,Linlin Hou

Cell communication and signaling : CCS 22:247 PubMed38689280

2024

The c-Abl-RACK1-FAK signaling axis promotes renal fibrosis in mice through regulating fibroblast-myofibroblast transition.

Applications

Unspecified application

Species

Unspecified reactive species

Qianyi Bao,Anyu Wang,Wenxuan Hong,Yushu Wang,Baojie Li,Lin He,Xiaodong Yuan,Gang Ma

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2303291 PubMed37553778

2023

Lepr-Expressing PDLSCs Contribute to Periodontal Homeostasis and Respond to Mechanical Force by Piezo1.

Applications

Unspecified application

Species

Unspecified reactive species

Danting Zhang,Weimin Lin,Shuang Jiang,Peng Deng,Linfeng Liu,Qian Wang,Rui Sheng,Hui Sophie Shu,Lijun Wang,Weiguo Zou,Bo O Zhou,Junjun Jing,Ling Ye,Bo Yu,Shiwen Zhang,Quan Yuan

Annals of the New York Academy of Sciences 1518:299-314 PubMed36303271

2022

Epidermal growth factor activates a hypoxia-inducible factor 1α-microRNA-21 axis to inhibit aquaporin 4 in chronic rhinosinusitis.

Applications

Unspecified application

Species

Unspecified reactive species

Junjun Chen,Dong Liu,Jingpu Yang,Chengxun Jin,Chang Zhao,Jinzhang Cheng

Life (Basel, Switzerland) 12: PubMed36295111

2022

Distinguished Functions of Microglia in the Two Stages of Oxygen-Induced Retinopathy: A Novel Target in the Treatment of Ischemic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyi Zhou,Yutong Jing,Yali Niu,Tianfang Chang,Jiaxing Sun,Changmei Guo,Yusheng Wang,Guorui Dou

Nature communications 13:1487 PubMed35347124

2022

Cell death-induced immunogenicity enhances chemoimmunotherapeutic response by converting immune-excluded into T-cell inflamed bladder tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Fotis Nikolos,Kazukuni Hayashi,Xen Ping Hoi,Mark Ellie Alonzo,Qianxing Mo,Armine Kasabyan,Hideki Furuya,Jane Trepel,Dolores Di Vizio,Jlenia Guarnerio,Dan Theodorescu,Charles Rosser,Andrea Apolo,Matthew Galsky,Keith Syson Chan

Frontiers in cell and developmental biology 9:630262 PubMed34660566

2021

Pancreatic Cancer Progression Is Regulated by IPO7/p53/LncRNA MALAT1/MiR-129-5p Positive Feedback Loop.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Xu,Weixue Xu,Yang Xuan,Zhen Liu,Qinyun Sun,Cheng Lan

Translational oncology 14:101171 PubMed34243012

2021

ORMDL1 is upregulated and associated with favorable outcomes in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Wang,Wanjun Liu,Si Chen,Qianxin Luo,Yichen Li,Shaoyong Peng,Huaiming Wang,Xiaoxia Liu,Daici Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (AB184705)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (AB184705)

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (AB184705)

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (AB184705)

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (AB184705)

Western blot - Anti-Alpha Skeletal Muscle Actin antibody [EPR18430] - Loading Control (AB184705)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

Primary Antibodies

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise