AB52218

Anti-Alpha Skeletal Muscle Actin antibody - Loading Control

Name: Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (ab52218) | Abcam
Brand: Abcam Limited
SKU: AB52218
Price: 525 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(12 Publications)

Rabbit Polyclonal alpha skeletal muscle Actin antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human ACTA1.

View Alternative Names

ACTA, ACTA1, Alpha-actin-1

3 Images

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (AB52218)

ab52218 staining human skeletal muscle tissue. Staining is localised to cytoplasm.
Left panel : with primary antibody at 2 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (AB52218)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling Alpha Skeletal Muscle Actin with ab52218. Right pane - treated with immunizing peptide.

Unknown

Western blot - Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (AB52218)

All lanes:

Western blot - Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (ab52218) at 1/500 dilution

Lane 1:

extracts from LOVO cells,

Lane 2:

extracts from LOVO cells, with immunizing peptide

Predicted band size: 42 kDa

Observed band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ACTA1. The exact immunogen used to generate this antibody is proprietary information.

P68133

Specificity

Actin alpha 1 antibody detects endogenous levels of total Actin alpha 1 protein

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Skeletal Muscle Actin also known as Actin Alpha 1 Actin Alpha and Actin Protein is a highly conserved protein important for muscle function. It has a molecular mass of approximately 42 kDa. This protein is expressed in skeletal muscle tissues and is an essential component of muscle cell's cytoskeleton. The actin family to which Alpha Skeletal Muscle Actin belongs is integral for maintaining cell shape integrity and movement. This isoform of actin is especially significant in forming thin filaments in muscle fibers which are essential for muscle contraction.

Biological function summary

Alpha Skeletal Muscle Actin plays an essential role in the contractile apparatus of muscle cells. It exists as part of the actin filament complex interacting closely with myosin motor proteins. This interaction powers the sliding mechanism that enables muscle contraction and relaxation. Alpha-actin is responsible for mechanical stiffness and tension within muscle tissues enabling muscle fibers to withstand physical stress. The protein's structural role in muscle cells makes it indispensable for proper muscle function and muscle fiber stability.

Pathways

Alpha Skeletal Muscle Actin is deeply involved in the cytoskeletal organization and muscle contraction pathways. It is pivotal in the actin-myosin interaction pathway where it binds to troponin and tropomyosin which regulate muscle contraction cycles. Within these pathways the protein ensures coordinated muscle contractions alongside other proteins like myosin heavy chain and tropomyosin. These interactions coordinate muscle contraction maintaining muscle tone and health.

Alpha Skeletal Muscle Actin mutations have a direct link to various myopathies including actin myopathy and nemaline myopathy. These conditions often result in muscle weakness and occasionally muscle degeneration. The protein's malfunction may also connect to cardiomyopathies due to its important role in muscle function. Research suggests that protein interactions with dystrophin another muscle-associated protein might further elucidate its role in these muscle disorders. Actin dysfunction in these conditions highlights the necessity for healthy actin filaments for skeletal muscle integrity and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Advanced healthcare materials 12:e2301335 PubMed37499214

2023

Creating and Transferring an Innervated, Vascularized Muscle Flap Made from an Elastic, Cellularized Tissue Construct Developed In Situ.

Applications

Unspecified application

Species

Unspecified reactive species

Hideyoshi Sato,Keishi Kohyama,Takafumi Uchibori,Keisuke Takanari,Johnny Huard,Stephen F Badylak,Antonio D'Amore,William R Wagner

Cellular and molecular gastroenterology and hepatology 15:1475-1504 PubMed36801449

2023

Periostin Protects Against Alcohol-related Liver Disease by Activating Autophagy by Interacting With Protein Disulfide Isomerase.

Applications

Unspecified application

Species

Unspecified reactive species

Yanfei Zhang,Jiayu Jin,Heming Wu,Jingwen Huang,Shuting Ye,Jinhua Qiu,Gaoliang Ouyang,Tiantian Wu,Fan Liu,Yingfu Liu

The Journal of pathology 255:212-223 PubMed34228359

2021

Periostin deficiency reduces diethylnitrosamine-induced liver cancer in mice by decreasing hepatic stellate cell activation and cancer cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjun Xiao,Yezhen Zhang,Zhaofeng Li,Bin Liu,Dan Cui,Fan Liu,Dafan Chen,Yingfu Liu,Gaoliang Ouyang

Mediators of inflammation 2021:6639252 PubMed33927570

2021

Toll-Like Receptor 4 Mediated Oxidized Low-Density Lipoprotein-Induced Foam Cell Formation in Vascular Smooth Muscle Cells via Src and Sirt1/3 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongli Chen,Qiqi Xue,Lijuan Cao,Yanpin Wang,Yuanyuan Chen,Xiaojie Zhang,Fan Xiao,Ying Yang,Melvin R Hayden,Yan Liu,Ke Yang

Journal of applied physiology (Bethesda, Md. : 1985) 128:545-553 PubMed31999526

2020

The proarrhythmic features of pathological cardiac hypertrophy in neonatal rat ventricular cardiomyocyte cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Zeinab Neshati,Martin J Schalij,Antoine A F de Vries

Cell reports 30:793-806.e6 PubMed31968254

2020

Periostin Promotes Colorectal Tumorigenesis through Integrin-FAK-Src Pathway-Mediated YAP/TAZ Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Handong Ma,Jing Wang,Xueli Zhao,Tiantian Wu,Zhengjie Huang,Dafan Chen,Yingfu Liu,Gaoliang Ouyang

eLife 8: PubMed31549961

2019

A mechanism in agrin signaling revealed by a prevalent Rapsyn mutation in congenital myasthenic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Guanglin Xing,Hongyang Jing,Lei Zhang,Yu Cao,Lei Li,Kai Zhao,Zhaoqi Dong,Wenbing Chen,Hongsheng Wang,Rangjuan Cao,Wen-Cheng Xiong,Lin Mei

EBioMedicine 43:513-524 PubMed31076347

2019

CD5L is a pleiotropic player in liver fibrosis controlling damage, fibrosis and immune cell content.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Bárcena,Gemma Aran,Luís Perea,Lucía Sanjurjo,Érica Téllez,Anna Oncins,Helena Masnou,Isabel Serra,Mónica García-Gallo,Leonor Kremer,Margarita Sala,Carolina Armengol,Pau Sancho-Bru,Maria-Rosa Sarrias

The American journal of pathology 186:1568-81 PubMed27102768

2016

Treatment with ActRIIB-mFc Produces Myofiber Growth and Improves Lifespan in the Acta1 H40Y Murine Model of Nemaline Myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Tinklenberg,Hui Meng,Lin Yang,Fujun Liu,Raymond G Hoffmann,Mahua Dasgupta,Kenneth P Allen,Alan H Beggs,Edna C Hardeman,R Scott Pearsall,Robert H Fitts,Michael W Lawlor

European journal of endocrinology 174:389-98 PubMed26671973

2015

A nontargeted study of muscle proteome in severely obese women with androgen excess compared with severely obese men and nonhyperandrogenic women.

Applications

Unspecified application

Species

Unspecified reactive species

María Insenser,Rafael Montes-Nieto,M Ángeles Martínez-García,Héctor F Escobar-Morreale

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alpha Skeletal Muscle Actin antibody - Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (AB52218)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (AB52218)

Western blot - Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (AB52218)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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