AB97373

Anti-Alpha Skeletal Muscle Actin antibody - Loading Control

Name: Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (ab97373) | Abcam
Brand: Abcam Limited
SKU: AB97373
Price: 525 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal alpha skeletal muscle Actin antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACTA1 aa 1-150.

View Alternative Names

ACTA, ACTA1, Alpha-actin-1

3 Images

Unknown

Western blot - Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (AB97373)

10% SDS PAGE

All lanes:

Western blot - Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (ab97373) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 42 kDa

false

Supplier Data

Western blot - Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (AB97373)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (ab97373) at 1/5000 dilution

All lanes:

RD Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 42 kDa

false

Supplier Data

Western blot - Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (AB97373)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (ab97373) at 1/5000 dilution

Lane 1:

NIH-3T3 whole cell extract at 30 µg

Lane 2:

JC whole cell extract at 30 µg

Lane 3:

BCL-1 whole cell extract at 30 µg

Lane 4:

C2C12 whole cell extract at 30 µg

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

applications

Immunogen

Recombinant Fragment Protein within Human ACTA1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P68133

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Skeletal Muscle Actin also known as Actin Alpha 1 Actin Alpha and Actin Protein is a highly conserved protein important for muscle function. It has a molecular mass of approximately 42 kDa. This protein is expressed in skeletal muscle tissues and is an essential component of muscle cell's cytoskeleton. The actin family to which Alpha Skeletal Muscle Actin belongs is integral for maintaining cell shape integrity and movement. This isoform of actin is especially significant in forming thin filaments in muscle fibers which are essential for muscle contraction.

Biological function summary

Alpha Skeletal Muscle Actin plays an essential role in the contractile apparatus of muscle cells. It exists as part of the actin filament complex interacting closely with myosin motor proteins. This interaction powers the sliding mechanism that enables muscle contraction and relaxation. Alpha-actin is responsible for mechanical stiffness and tension within muscle tissues enabling muscle fibers to withstand physical stress. The protein's structural role in muscle cells makes it indispensable for proper muscle function and muscle fiber stability.

Pathways

Alpha Skeletal Muscle Actin is deeply involved in the cytoskeletal organization and muscle contraction pathways. It is pivotal in the actin-myosin interaction pathway where it binds to troponin and tropomyosin which regulate muscle contraction cycles. Within these pathways the protein ensures coordinated muscle contractions alongside other proteins like myosin heavy chain and tropomyosin. These interactions coordinate muscle contraction maintaining muscle tone and health.

Alpha Skeletal Muscle Actin mutations have a direct link to various myopathies including actin myopathy and nemaline myopathy. These conditions often result in muscle weakness and occasionally muscle degeneration. The protein's malfunction may also connect to cardiomyopathies due to its important role in muscle function. Research suggests that protein interactions with dystrophin another muscle-associated protein might further elucidate its role in these muscle disorders. Actin dysfunction in these conditions highlights the necessity for healthy actin filaments for skeletal muscle integrity and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5677 PubMed40593809

2025

Regenerating aged bone marrow via a nitric oxide nanopump.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Li,Sihan Hu,Hanwen Li,Wenzheng Lin,Duoyi Zhao,Zhuobin Xu,Chun Pan,Huihui Wang,Dandan Li,Jingjing Liu,Hao Chen

Nature food 3:74-85 PubMed37118488

2022

A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Messmer,Iva Klevernic,Carolina Furquim,Ekaterina Ovchinnikova,Arin Dogan,Helder Cruz,Mark J Post,Joshua E Flack

Molecular therapy. Nucleic acids 19:1290-1298 PubMed32092825

2020

Allele-Selective Knockdown of MYH7 Using Antisense Oligonucleotides.

Applications

Unspecified application

Species

Unspecified reactive species

Brian R Anderson,Marianne L Jensen,Peter H Hagedorn,Sean C Little,Richard E Olson,Ron Ammar,Bernadette Kienzle,John Thompson,Ivar McDonald,Stephen Mercer,Jonas Vikesaa,Bettina Nordbo,Larry Iben,Yang Cao,Joanne Natale,Greg Dalton-Kay,Angela Cacace,Bo R Hansen,Maj Hedtjärn,Troels Koch,Linda J Bristow

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alpha Skeletal Muscle Actin antibody - Loading Control

Western blot - Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (AB97373)

Western blot - Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (AB97373)

Western blot - Anti-Alpha Skeletal Muscle Actin antibody - Loading Control (AB97373)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise