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Rabbit Recombinant Monoclonal alpha smooth muscle Actin acetyl E3 antibody. Suitable for IHC-Fr, IHC-P, WB, ICC/IF, Flow Cyt (Intra), ELISA and reacts with Mouse, Rat, Human, Synthetic peptide samples. Cited in 583 publications.
Alternative names=Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTSA, ACTVS, ACTA2, GIG46
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-Fr | IP | IHC-P | WB | ICC/IF | Flow Cyt (Intra) | ELISA | |
|---|---|---|---|---|---|---|---|
| Human | Expected | Not recommended | Tested | Tested | Tested | Tested | Expected |
| Mouse | Expected | Not recommended | Tested | Tested | Tested | Expected | Expected |
| Rat | Expected | Not recommended | Expected | Tested | Tested | Expected | Expected |
| Synthetic peptide | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
Images
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse, Synthetic peptide | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200 - 1/500 | Notes Internal test showed non-specific staining on mouse kidney, mouse stomach, rat kidney and rat stomach. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/200 - 1/500 | Notes Internal test showed non-specific staining on mouse kidney, mouse stomach, rat kidney and rat stomach. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/200 - 1/500 | Notes Internal test showed non-specific staining on mouse kidney, mouse stomach, rat kidney and rat stomach. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 - 1/5000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes - |
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Species Rat | Dilution info 1/500 | Notes - |
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 0.00002-1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Recommended products
Rabbit Recombinant Monoclonal alpha smooth muscle Actin acetyl E3 antibody. Suitable for IHC-Fr, IHC-P, WB, ICC/IF, Flow Cyt (Intra), ELISA and reacts with Mouse, Rat, Human, Synthetic peptide samples. Cited in 583 publications.
Alternative names=Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTSA, ACTVS, ACTA2, GIG46
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
- Clone number
E184
- Purification technique
Affinity purification Protein A
- Specificity
For immunohistochemistry, this antibody only detects actin in smooth muscle and not cardiac muscle.
This antibody has been shown to detect P62736-ACTA (gene ACTA2): (Ac)-EEEDSTALVC and P63267-ACTH (gene ACTG2): (Ac)-EEETTALVC in indirect ELISA.- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
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