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Rabbit Recombinant Monoclonal alpha smooth muscle Actin acetyl E3 antibody. Carrier free. Suitable for ELISA, IHC-P, ICC/IF, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Synthetic peptide, Human, Mouse, Rat samples. Cited in 26 publications.
Alternative names=Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTSA, ACTVS, ACTA2, GIG46
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
The exact immunogen used to generate this antibody is proprietary information.
ELISA | IHC-P | ICC/IF | WB | IHC-Fr | IP | Flow Cyt (Intra) | |
---|---|---|---|---|---|---|---|
Human | Tested | Tested | Tested | Tested | Expected | Not recommended | Tested |
Mouse | Tested | Tested | Tested | Expected | Expected | Not recommended | Expected |
Rat | Tested | Expected | Tested | Tested | Expected | Not recommended | Expected |
Synthetic peptide | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species Human | Dilution info 0.00002-1 µg/mL | Notes - |
Species Mouse | Dilution info 0.00002-1 µg/mL | Notes - |
Species Rat | Dilution info 0.00002-1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Internal test showed non-specific staining on mouse kidney, mouse stomach, rat kidney and rat stomach. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Internal test showed non-specific staining on mouse kidney, mouse stomach, rat kidney and rat stomach. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes Internal test showed non-specific staining on mouse kidney, mouse stomach, rat kidney and rat stomach. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes ab199376- Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Rabbit Recombinant Monoclonal alpha smooth muscle Actin acetyl E3 antibody. Carrier free. Suitable for ELISA, IHC-P, ICC/IF, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Synthetic peptide, Human, Mouse, Rat samples. Cited in 26 publications.
Alternative names=Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTSA, ACTVS, ACTA2, GIG46
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
The exact immunogen used to generate this antibody is proprietary information.
Yes
E184
Affinity purification Protein A
For immunohistochemistry, this antibody only detects actin in smooth muscle and not cardiac muscle.
This antibody has been shown to detect P62736-ACTA (gene ACTA2): (Ac)-EEEDSTALVC and P63267-ACTH (gene ACTG2): (Ac)-EEETTALVC in indirect ELISA.
Blue Ice
+4°C
Do Not Freeze
ab215368 is the carrier-free version of ab32575.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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