Rabbit Recombinant Monoclonal alpha smooth muscle Actin acetyl E3 antibody. Carrier free. Suitable for ELISA, IHC-P, ICC/IF, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Synthetic peptide, Mouse, Human, Rat samples. Cited in 26 publications.
pH: 7.2 - 7.4
Constituents: PBS
ELISA | IHC-P | ICC/IF | WB | IHC-Fr | IP | Flow Cyt (Intra) | |
---|---|---|---|---|---|---|---|
Human | Expected | Tested | Tested | Tested | Expected | Not recommended | Tested |
Mouse | Expected | Tested | Tested | Tested | Expected | Not recommended | Expected |
Rat | Expected | Expected | Tested | Tested | Expected | Not recommended | Expected |
Synthetic peptide | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Internal test showed non-specific staining on mouse kidney, mouse stomach, rat kidney and rat stomach. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Internal test showed non-specific staining on mouse kidney, mouse stomach, rat kidney and rat stomach. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes Internal test showed non-specific staining on mouse kidney, mouse stomach, rat kidney and rat stomach. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes ab199376- Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
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Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
Rabbit Recombinant Monoclonal alpha smooth muscle Actin acetyl E3 antibody. Carrier free. Suitable for ELISA, IHC-P, ICC/IF, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Synthetic peptide, Mouse, Human, Rat samples. Cited in 26 publications.
pH: 7.2 - 7.4
Constituents: PBS
For immunohistochemistry, this antibody only detects actin in smooth muscle and not cardiac muscle.
This antibody has been shown to detect P62736-ACTA (gene ACTA2): (Ac)-EEEDSTALVC and P63267-ACTH (gene ACTG2): (Ac)-EEETTALVC in indirect ELISA.
ab215368 is the carrier-free version of Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Alpha smooth muscle Actin also known as ACTG2 is an important protein in muscle contraction and cellular structure. It is most commonly found in smooth muscle cells and is not typically expressed in striated muscle. This protein is part of the actin family and has a molecular mass of approximately 42 kDa. ACTG2 plays a role in forming microfilaments which are key components of the muscle contractile machinery. In smooth muscle tissues such as those found in the intestines and blood vessels ACTG2 is often detected together with other cytoskeletal and regulatory proteins.
ACTG2 contributes to muscle contraction by facilitating the interaction between actin and myosin. It is not considered a part of a protein complex but interacts closely with several other proteins involved in contractile functions. Beyond contraction ACTG2 also supports cellular integrity and shape maintenance. In tissues ACTG2 is involved in activities that require dynamic structural reorganization such as during cellular migration and wound healing highlighting its versatility beyond just a contractile agent.
ACTG2 participates in the regulation of the smooth muscle contraction pathway influencing muscle tone and responsiveness to stimuli. The actin-myosin interaction where ACTG2 is involved is critical for the contractile process with other proteins like myosin light chain kinase (MLCK) and tropomyosin playing supportive roles. Additionally the integrin signaling pathway is another context where ACTG2 is indirectly involved aiding in the transmission of mechanical signals from the extracellular matrix to the cell.
ACTG2 is associated with conditions such as megacystis-microcolon-intestinal hypoperistalsis syndrome (MMIHS) and various vascular disorders. Mutations in the ACTG2 gene can disrupt normal muscle function leading to defective peristalsis in the gastrointestinal tract as seen in MMIHS. The connection between ACTG2 and myosin regulatory light chains illustrates how changes in its function can impact muscle contractility and lead to disease. In vascular disorders abnormal expression of ACTG2 is often linked to impaired vessel tone and blood flow regulation.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
FoxA1 (red) and alpha smooth muscle actin (green) staining are shown by indirect immunofluorescence on sections of prostate from mice of the indicated genotypes.
Wild type: 21 weeks, Tgfbr2r/r: 44 weeks, Ptenr/r: 21 weeks, Ptenr/r;Tgfbr2r/r: 11 weeks, Apcr/r: 36 weeks, and Apcr/r;Tgfbr2r/r: 24 weeks old.
IF images were captured on an Olympus BX51 microscope and DP70 digital camera, or on a Nikon Eclipse NI-U and captured with a DS-QI1 camera with NIS Elements software.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Clone E184 (ab215368) has been successfully conjugated by Abcam. This image was generated using Anti-alpha smooth muscle Actin antibody [E184] (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab196919 for protocol details.
Alexa Fluor® 647 Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab196919 staining alpha Smooth Muscle Actin in HeLa cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 647 Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab196919 at 1/100 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Intracellular Flow Cytometry analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling alpha smooth muscle Actin with purified Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575 at a dilution of 1/20 (red).
Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabeled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse smooth muscle tissue labeling alpha smooth muscle Actin with purified Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575 at a dilution of 1/200. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).
Negative control using PBS instead of primary antibody.
Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575).
Clone E184 (ab215368) has been successfully conjugated by Abcam. This image was generated using Anti-alpha smooth muscle Actin antibody [E184] (PE). Please refer to PE Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab209435 for protocol details.
PE Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab209435 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with PE Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab209435 at 1/500 dilution (Pseudocolored in green) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This data was developed using Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575, the same antibody clone in a different buffer formulation. Different batches of Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575 were tested on A431 (Human epidermoid carcinoma epithelial cell) lysate at 0.005 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 42 kDa.
All lanes: Western blot - Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575)
This IHC data was generated using the same anti-alpha smooth muscle Actin antibody clone, E184, in a different buffer formulation (cat# Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human smooth muscle tissue labelling alpha smooth muscle Actin with purified Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575 at a dilution of 1/200. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody
Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma) cells labeling alpha smooth muscle Actin (green) with purified Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, anti-Tubulin (mouse mAb) at 1/1000 followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Alexa Fluor®594 goat anti-mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).
For negative control 1, rabbit primary antibody and anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) were used. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse primary antibody) was used followed by anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575).
Following reconstitution in PBS with 10% DMSO, peptides (1 ug per mL) were immobilised in PBS on an ELISA plate overnight. After blocking in 5% BSA, primary antibody (Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein ab271180) was added in a concentration range of 0.017-1000 ng per mL.
Pre-adsorbed secondary antibody goat anti-rabbit IgG H&L (HRP, Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) was used at 1/20000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575).
Following reconstitution in PBS with 10% DMSO, peptides (1 ug per mL) were immobilised in PBS on an ELISA plate overnight. After blocking in 5% BSA, primary antibody (Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein ab271180) was added in a concentration range of 0.017-1000 ng per mL.
Pre-adsorbed secondary antibody goat anti-rabbit IgG H&L (HRP, Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) was used at 1/20000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575).
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