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Rabbit Recombinant Monoclonal alpha smooth muscle Actin acetyl E3 antibody. Carrier free. Suitable for ELISA, IHC-P, ICC/IF, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Synthetic peptide, Human, Mouse, Rat samples. Cited in 26 publications.

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Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAIHC-PICC/IFWBIHC-FrIPFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Expected
Not recommended
Tested
Mouse
Tested
Tested
Tested
Expected
Expected
Not recommended
Expected
Rat
Tested
Expected
Tested
Tested
Expected
Not recommended
Expected
Synthetic peptide
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Images

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Species
Human
Dilution info
0.00002-1 µg/mL
Notes

-

Species
Mouse
Dilution info
0.00002-1 µg/mL
Notes

-

Species
Rat
Dilution info
0.00002-1 µg/mL
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Internal test showed non-specific staining on mouse kidney, mouse stomach, rat kidney and rat stomach.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species
Human
Dilution info
-
Notes

Internal test showed non-specific staining on mouse kidney, mouse stomach, rat kidney and rat stomach.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
-
Notes

Internal test showed non-specific staining on mouse kidney, mouse stomach, rat kidney and rat stomach.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Rat, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab199376- Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Target data

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

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Rabbit Recombinant Monoclonal alpha smooth muscle Actin acetyl E3 antibody. Carrier free. Suitable for ELISA, IHC-P, ICC/IF, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Synthetic peptide, Human, Mouse, Rat samples. Cited in 26 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Carrier free

Yes

Clone number

E184

Purification technique

Affinity purification Protein A

Specificity

For immunohistochemistry, this antibody only detects actin in smooth muscle and not cardiac muscle.
This antibody has been shown to detect P62736-ACTA (gene ACTA2): (Ac)-EEEDSTALVC and P63267-ACTH (gene ACTG2): (Ac)-EEETTALVC in indirect ELISA.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab215368 is the carrier-free version of ab32575.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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