Anti-alpha smooth muscle Actin antibody [1A4]

14 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

  Alpha smooth muscle Actin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of human colon using mouse Anti-alpha smooth muscle Actin antibody

  Immunohistochemical analysis of formalin fixed paraffin embedded human colon labelling alpha smooth muscle actin with ab7817 at a concentration of 0.01 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab7817 anti-alpha smooth muscle actin antibody [1A4] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

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  Flow Cytometry (Intracellular) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

  Alpha smooth muscle Actin Flow Cytometry (Intracellular) staining of SV40LT-SMC cells using mouse Anti-alpha smooth muscle Actin antibody

  Overlay histogram showing SV40LT-SMC cells stained with ab7817 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab7817 1.137μg/ml) for 30 min at 22°C. The secondary antibody used was Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution for 30 min at 22°C

  Isotype control antibody (black line) was mouse IgG2a [18C8BC7AD10] (Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control ab170191) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

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  This image is courtesy of an anonymous customer review

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

  Alpha smooth muscle Actin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of mouse aorta and skin using mouse Anti-alpha smooth muscle Actin antibody

  Immunohistochemical analysis of mouse aorta (A) or skin (B) tissue staining alpha smooth muscle Actin with ab7817.
  
  Tissue was fixed with 10% Neutral Buffered Formalin and blocked with 1% serum for 45 minutes 21°C; antigen retrieval was by enzymatic method in 0.0001% Trypsin-CaCl. Samples were incubated with primary antibody (0.034μg/ml in 0.3% Triton X-100 in PBS) for 1 hour at 21°C. A biotin-conjugated horse anti-mouse polyclonal IgG (1/50 dilution) was used as the secondary antibody.

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  This image is courtesy of a customer review submitted by Dr. Ho-Jae Lee

  Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

  Alpha smooth muscle Actin Immunocytochemistry/ Immunofluorescence staining of mouse heart cells using mouse Anti-alpha smooth muscle Actin antibody

  ab7817 staining alpha smooth muscle Actin in mouse heart cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde permeabilized with TritonX-100 and blocked with 5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody 6.82μg/ml in blocking buffer for 2 hours. An Alexa Fluor^® 488-conjugated Donkey monoclonal to mouse IgG dilution 1/200 was used as secondary antibody.

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  This image is courtesy of a customer review submitted by Rudolf Jung

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

  Alpha smooth muscle Actin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human liver tissue using mouse Anti-alpha smooth muscle Actin antibody

  ab7817 staining alpha smooth muscle actin in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized with wash buffer with tween; antigen retrieval was by heat mediation in Tris-EDTA buffer pH 9.0. Samples were incubated with primary antibody (0.034μg/ml in blocking buffer) for 30 minutes at 20°C. A HRP-conjugated Goat anti-mouse IgG polyclonal (undiluted) was used as the secondary antibody.

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  Western blot - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

  Gel type: MOPS
  

  Blocking buffer: 3% milk

  Loading control: alpha tubulin (Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866), secondary Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (1:10000 dilution)

  All lanes: Western blot - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817) at 1 µg/mL

  Lane 1: NIH 3T3 whole cell lysate at 20 µg

  Lane 2: SV40LT-SMC whole cell lysate at 20 µg

  Lane 3: A431 whole cell lysate at 20 µg

  Lane 4: A549 whole cell lysate at 20 µg

  Lane 5: Jurkat whole cell lysate at 20 µg

  Secondary

  All lanes: Goat anti-Mouse IgG H&L (IRDye® 800RD) at 1/10000 dilution

  Predicted band size: 42 kDa

  Observed band size: 42 kDa

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  Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

  Alpha smooth muscle Actin Immunocytochemistry/ Immunofluorescence staining of SV40LT-SMC cells using mouse Anti-alpha smooth muscle Actin antibody

  ab7817 staining alpha smooth muscle Actin in SV40LT-SMC cells (positive control top panel) and A431 cells (negative control bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab7817 at 1μg/ml concentration and Anti-beta Tubulin antibody - Loading Control ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor^® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor^® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
  Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

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  Western blot - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

  Gel type: MOPS
  

  Blocking buffer: 3% milk

  Loading control: alpha tubulin (Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866), secondary Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (1:10000 dilution)

  All lanes: Western blot - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817) at 1 µg/mL

  Lane 1: Human colon tissue lysate at 20 µg

  Lane 2: Mouse colon tissue lysate at 20 µg

  Lane 3: Human Foreskin Fibroblast Whole Cell Lysate at 20 µg

  Secondary

  All lanes: Goat anti-Mouse IgG H&L (IRDye® 800RD) at 1/10000 dilution

  Predicted band size: 42 kDa

  Observed band size: 42 kDa

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  This image is courtesy of an anonymous customer review

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

  Alpha smooth muscle Actin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-alpha smooth muscle Actin antibody

  ab7817 staining alpha smooth muscle actin in Mouse intestine tissue by Immunohistochemistry-Immunofluorescence. Tissue was fixed with formaldehyde and blocked with 100% Cas-block for 30 minutes at room temperature; antigen retrieval was performed by heat mediated citrate buffer, pH6. The sample was incubated with primary antibody at 0.034μg/ml for 16 hours at 4°C. An Alexa Fluor^® 488 Goat anti-mouse IgG was used as the secondary antibody at 1/400 dilution. Autofluorescence was blocked with 0.1% Sudan Black in 70% ethanol for 10 minutes at room temperature after antigen retrieval, and followed with 3X wash with PBS-T after antigen retrieval. Image was taken with confocal microscope.

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  This image is courtesy of a customer review submitted by Charles Pallangyo

  Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

  Alpha smooth muscle Actin Immunocytochemistry/ Immunofluorescence staining of mouse primary colon myofibroblasts using mouse Anti-alpha smooth muscle Actin antibody

  ab7817 staining alpha smooth muscle Actin (green) in Mouse primary colon myofibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% BSA for 30 hours at 25°C. Samples were incubated with primary antibody (1/100 dilution in PBS + 5% BSA) for 2 hours at 25°C. Donkey Anti-Mouse IgG H&L (DyLight^® 488) (Donkey Anti-Mouse IgG H&L (DyLight® 488) ab96875) (1/1000 dilution) was used as the secondary antibody. Costained with Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547 Rabbit anti-Vimentin (red).

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  This image is courtesy of an anonymous customer review.

  Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

  Alpha smooth muscle Actin Immunocytochemistry/ Immunofluorescence staining of IMR-90 cells using mouse Anti-alpha smooth muscle Actin antibody

  ab7817 staining alpha smooth muscle Actin in human IMR-90 (Human Lung Fibroblast Cell Line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde permeabilized with 0.1% TritonX-100 and blocked with 100% Cad-Block for 30 minutes at room temperature. Samples were incubated with primary antibody 3.41μg/ml in antibody diluent buffer for 16 hours at 4°C. An Alexa Fluor^® 488-conjugated polyclonal Goat anti-mouse IgG dilution 1/400 was used as secondary antibody.

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  Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

  Alpha smooth muscle Actin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-alpha smooth muscle Actin antibody

  ab7817 staining ACTA2 in NIH3T3 WT and NIH3T3-ACTA2 KO cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal rabbit serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7817 at 1µg/ml (shown in green) and Rabbit polyclonal to beta Tubulin - Loading Control (Anti-beta Tubulin antibody - Loading Control ab6046) (shown in pseudocolour magenta). Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).

  Also suitable in cells fixed with 100% methanol (5 min).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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  This image is courtesy of a customer review submitted by David Ivancic

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

  Alpha smooth muscle Actin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of 10% NBF-fixed human colon tissue using mouse Anti-alpha smooth muscle Actin antibody

  Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of 10% NBF-fixed human colon tissue permeabilized with 0.05% tween 20. Stained with ab7817 at 1/100 dilution. Secondary antibody used was Alexa fluor® 488 Donkey anti-Rabbit IgG at 1/300 dilution. Blocking was done with Sea Block for 30 minutes at 22°C. The sample was incubated with the primary antibody and Sea Block for 14 hours at 4°C. Antigen retrieval method was heat mediated, ab94674 100X Citrate Buffer pH 6.0.

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  Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

  Alpha smooth muscle Actin Immunocytochemistry/ Immunofluorescence staining of SaOS-2 cells using mouse Anti-alpha smooth muscle Actin antibody

  ab7817 staining ACTA2 in SaOS-2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal rabbit serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7817 at 1µg/ml (shown in green) and Rabbit polyclonal to beta Tubulin - Loading Control (Anti-beta Tubulin antibody - Loading Control ab6046) (shown in pseudocolour magenta). Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.