Mouse Recombinant Monoclonal alpha smooth muscle Actin antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 9 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (AB240654), expandable thumbnail](https://content.abcam.com/products/images/alpha-smooth-muscle-actin-antibody-1a4-bsa-and-azide-free-ab240654--immunocytochemistry-immunofluorescence-img172586.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (AB240654)")
![Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (AB240654), expandable thumbnail](https://content.abcam.com/products/images/alpha-smooth-muscle-actin-antibody-1a4-bsa-and-azide-free-ab240654--immunocytochemistry-immunofluorescence-img173611.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (AB240654)")
![Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (AB240654), expandable thumbnail](https://content.abcam.com/products/images/alpha-smooth-muscle-actin-antibody-1a4-bsa-and-azide-free-ab240654--immunocytochemistry-immunofluorescence-img175005.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (AB240654)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (AB240654), expandable thumbnail](https://content.abcam.com/products/images/alpha-smooth-muscle-actin-antibody-1a4-bsa-and-azide-free-ab240654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img173353.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (AB240654)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (AB240654), expandable thumbnail](https://content.abcam.com/products/images/alpha-smooth-muscle-actin-antibody-1a4-bsa-and-azide-free-ab240654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img180942.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (AB240654)")
Publications
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- Journal of nanobiotechnology 22:142024Traditional Chinese medicine inspired dual-drugs loaded inhalable nano-therapeutics alleviated idiopathic pulmonary fibrosis by targeting early inflammation and late fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeiling Zheng,Kai Liu,Lei Li,Cuiling Feng,Guanghao WuPubMed 38166847
- FASEB bioAdvances 5:377-3942023A combination of 5/6-nephrectomy and unilateral ureteral obstruction model accelerates progression of remote organ fibrosis in chronic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyoka Homma,Yuki Enoki,Sato Uchida,Kazuaki Taguchi,Kazuaki MatsumotoPubMed 37810171
- Biology direct 18:352023Single-cell transcriptomics reveals cellular heterogeneity and macrophage-to-mesenchymal transition in bicuspid calcific aortic valve disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Lyu,Yang Liu,Binglin Li,Ran Xu,Jianghong Guo,Dan ZhuPubMed 37391760
- Annals of medicine 55:21892952023Targeting GPC3 cancer-associated fibroblasts sensitizing the PD-1 blockage therapy in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDinuo Li,Yu Wang,Ce Shi,Shuai Fu,Yi-Fei Sun,Chen LiPubMed 37036308
- Oncogenesis 12:182023Transgelin promotes lung cancer progression via activation of cancer-associated fibroblasts with enhanced IL-6 release.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChanjun Sun,Kaishang Zhang,Chen Ni,Jiajia Wan,Xixi Duan,Xiaohan Lou,Xiaohan Yao,Xiangnan Li,Ming Wang,Zhuoyu Gu,Pengyuan Yang,Zhenzhen Li,Zhihai QinPubMed 36990991
- Cell communication and signaling : CCS 20:1042022SENP1 regulates the transformation of lung resident mesenchymal stem cells and is associated with idiopathic pulmonary fibrosis progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Sun,Xiaoshu Liu,Xiaoyu Yang,Xiaoyan Jing,Chunyan Duan,Ganghao Yang,Chi Wu,Hui Huang,Qun Luo,Shu Xia,Qian Zhang,Yang Yang,Zuojun XuPubMed 35836260
- iScience 25:1039782022The role of long noncoding RNA Nron in atherosclerosis development and plaque stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng Du,Cheng Wang,Liuye Yang,Bing Liu,Zhe Zheng,Liu Yang,Fengxiao Zhang,Jiangtong Peng,Dan Huang,Kai HuangPubMed 35295812
- Cancer cell 39:1227-1244.e202021Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesColin Hutton,Felix Heider,Adrian Blanco-Gomez,Antonia Banyard,Alexander Kononov,Xiaohong Zhang,Saadia Karim,Viola Paulus-Hock,Dale Watt,Nina Steele,Samantha Kemp,Elizabeth K J Hogg,Joanna Kelly,Rene-Filip Jackstadt,Filipa Lopes,Matteo Menotti,Luke Chisholm,Angela Lamarca,Juan Valle,Owen J Sansom,Caroline Springer,Angeliki Malliri,Richard Marais,Marina Pasca di Magliano,Santiago Zelenay,Jennifer P Morton,Claus JørgensenPubMed 34297917
- Cells 10:2021Evogliptin Suppresses Calcific Aortic Valve Disease by Attenuating Inflammation, Fibrosis, and Calcification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBongkun Choi,Eun-Young Kim,Ji-Eun Kim,Soyoon Oh,Si-On Park,Sang-Min Kim,Hyuksu Choi,Jae-Kwan Song,Eun-Ju ChangPubMed 33401457
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Expected |
| Mouse | Expected | Tested | Tested | Expected |
| Rat | Tested | Tested | Tested | Tested |
| Baboon | Predicted | Predicted | Not recommended | Predicted |
| Cow | Predicted | Predicted | Not recommended | Predicted |
| Mammals | Predicted | Predicted | Not recommended | Predicted |
| Pig | Predicted | Predicted | Not recommended | Predicted |
| Rabbit | Predicted | Predicted | Not recommended | Predicted |
| Sheep | Predicted | Predicted | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/50000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Pig, Mammals, Baboon, Sheep | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Pig, Mammals, Baboon, Sheep | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Not recommended for Human. |
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rabbit, Cow, Pig, Mammals, Baboon, Sheep | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Pig, Mammals, Baboon, Sheep | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Alternative names
Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTSA, ACTVS, GIG46, ACTA2
Recommended products
Mouse Recombinant Monoclonal alpha smooth muscle Actin antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 9 publications.
Alternative names
Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTSA, ACTVS, GIG46, ACTA2
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
1A4
- Purification technique
Affinity purification Protein A
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Do Not Freeze
Notes
This product has switched to a recombinant production method on 15th September 2021.
ab240654 is the carrier-free version of Anti-alpha smooth muscle Actin antibody [1A4] ab7817.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.
- Biological function summary
Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.
- Pathways
Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.
- Associated diseases and disorders
Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654), expandable thumbnail](https://content.abcam.com/products/images/alpha-smooth-muscle-actin-antibody-1a4-bsa-and-azide-free-ab240654--immunocytochemistry-immunofluorescence-img172586.jpg "Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)")
Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)
alpha smooth muscle Actin immunofluorescence staining of SV40LT-SMC cells and A431 cells using mouse anti-alpha smooth muscle Actin antibody
This data was developed using the same antibody clone in a different buffer formulation (Anti-alpha smooth muscle Actin antibody [1A4] ab7817).
Anti-alpha smooth muscle Actin antibody [1A4] ab7817 staining alpha smooth muscle Actin in SV40LT-SMC cells (positive control top panel) and A431 cells (negative control bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Anti-alpha smooth muscle Actin antibody [1A4] ab7817 at 1μg/ml concentration and Anti-beta Tubulin antibody - Loading Control ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).![Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654), expandable thumbnail](https://content.abcam.com/products/images/alpha-smooth-muscle-actin-antibody-1a4-bsa-and-azide-free-ab240654--immunocytochemistry-immunofluorescence-img173611.jpg "Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)")
Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)
This data was developed using Anti-alpha smooth muscle Actin antibody [1A4] ab7817, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH 3T3 cells labelling alpha smooth muscle Actin with Anti-alpha smooth muscle Actin antibody [1A4] ab7817 at 1/50 dilution (Green). Confocal image showing cytoskeleton staining in NIH 3T3 cells. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed was used as a secondary at 1/10 dilution. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/20 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/50 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Negative control: secondary counterstain antibody only control.![Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654), expandable thumbnail](https://content.abcam.com/products/images/alpha-smooth-muscle-actin-antibody-1a4-bsa-and-azide-free-ab240654--immunocytochemistry-immunofluorescence-img175005.jpg "Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)")
Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)
This data was developed using Anti-alpha smooth muscle Actin antibody [1A4] ab7817, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH 3T3 cells labelling alpha smooth muscle Actin with Anti-alpha smooth muscle Actin antibody [1A4] ab7817 at 1/50 dilution (Green). Confocal image showing cytoskeleton staining in NIH 3T3 cells. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed was used as a secondary at 1/10 dilution. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/20 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/50 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Negative control: secondary counterstain antibody only control.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654), expandable thumbnail](https://content.abcam.com/products/images/alpha-smooth-muscle-actin-antibody-1a4-bsa-and-azide-free-ab240654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img173353.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)
Immunohistochemical analysis of Rat colon tissue labelling alpha smooth muscle Actin with Anti-alpha smooth muscle Actin antibody [1A4] ab7817 at 1/50000 dilution and ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) Secondary antibody. Positive cytoplasmic staining on Rat colon tissue is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
This image is produced using the same clone but in a different formulation Anti-alpha smooth muscle Actin antibody [1A4] ab7817.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654), expandable thumbnail](https://content.abcam.com/products/images/alpha-smooth-muscle-actin-antibody-1a4-bsa-and-azide-free-ab240654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img180942.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)
Immunohistochemical analysis of Mouse colon tissue labelling alpha smooth muscle Actin with Anti-alpha smooth muscle Actin antibody [1A4] ab7817 at 1/50000 dilution and ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) Secondary antibody. Positive cytoplasmic staining on Mouse colon tissue is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
This image is produced using the same clone but in a different formulation Anti-alpha smooth muscle Actin antibody [1A4] ab7817.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654), expandable thumbnail](https://content.abcam.com/products/images/alpha-smooth-muscle-actin-antibody-1a4-bsa-and-azide-free-ab240654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img184211.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)
Immunohistochemical analysis of Human colon tissue labelling alpha smooth muscle Actin with Anti-alpha smooth muscle Actin antibody [1A4] ab7817 at 1/50000 dilution and ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) Secondary antibody. Positive cytoplasmic staining on Human colon tissue is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
This image is produced using the same clone but in a different formulation Anti-alpha smooth muscle Actin antibody [1A4] ab7817.
![Western blot - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654), expandable thumbnail](https://content.abcam.com/products/images/alpha-smooth-muscle-actin-antibody-1a4-bsa-and-azide-free-ab240654--western-blot-img183622.jpg "Western blot - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)")
Western blot - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)
This image is produced using the same clone but in a different formulation Anti-alpha smooth muscle Actin antibody [1A4] ab7817.
All lanes: Western blot - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654) at 1/1000 dilution
Lane 1: NIH 3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 2: IMR-90 (human lung fibroblast), whole cell lysate at 20 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654), expandable thumbnail](https://content.abcam.com/products/images/alpha-smooth-muscle-actin-antibody-1a4-bsa-and-azide-free-ab240654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img184211.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)")
![Western blot - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654), expandable thumbnail](https://content.abcam.com/products/images/alpha-smooth-muscle-actin-antibody-1a4-bsa-and-azide-free-ab240654--western-blot-img183622.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)")
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