Anti-alpha smooth muscle Actin antibody (ab5694) is a rabbit polyclonal antibody detecting alpha smooth muscle Actin in Western Blot, IHC-P. Suitable for Human, Mouse.
- Over 3000 publications
- Trusted since 2003
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- Nature communications 15:36842024Development of Aptamer-DNAzyme based metal-nucleic acid frameworks for gastric cancer therapy.Applications:IHC-PReactive species:HumanJiaqi Yan,Rajendra Bhadane,Meixin Ran,Xiaodong Ma,Yuanqiang Li,Dongdong Zheng,Outi M H Salo-Ahen,Hongbo ZhangPubMed 38693181
- Disease models & mechanisms 17:2024eNOS plays essential roles in the developing heart and aorta linked to disruption of Notch signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLorraine Eley,Rachel V Richardson,Ahlam Alqahtani,Bill Chaudhry,Deborah J HendersonPubMed 38111957
- Renal failure 46:23007272024CXC chemokine receptor 7 ameliorates renal fibrosis by inhibiting β-catenin signaling and epithelial-to-mesenchymal transition in tubular epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPing Meng,Chunli Liu,Jingchun Li,Ping Fang,Bo Yang,Wei Sun,Yunfang ZhangPubMed 38189094
- Scientific reports 14:5592024WNTA5-mediated miR-374a-5p regulates vascular smooth muscle cell phenotype transformation and M1 macrophage polarization impacting intracranial aneurysm progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZengshi Li,Junqiang Huang,Lijian Yang,Xi Li,Wei LiPubMed 38177414
- Clinical and translational medicine 13:e15162023The molecular classification of cancer-associated fibroblasts on a pan-cancer single-cell transcriptional atlas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBonan Chen,Wai Nok Chan,Fuda Xie,Chun Wai Mui,Xiaoli Liu,Alvin H K Cheung,Raymond W M Lung,Chit Chow,Zhenhua Zhang,Canbin Fang,Peiyao Yu,Shihua Shi,Shikun Zhou,Guoming Chen,Zhangding Wang,Shouyu Wang,Xiaofan Ding,Bing Huang,Li Liang,Yujuan Dong,Chi Chun Wong,William K K Wu,Alfred S L Cheng,Nathalie Wong,Jun Yu,Kwok Wai Lo,Gary M K Tse,Wei Kang,Ka Fai ToPubMed 38148640
- eLife 12:2023Apoptosis recognition receptors regulate skin tissue repair in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlivia Justynski,Kate Bridges,Will Krause,Maria Fernanda Forni,Quan M Phan,Teresa Sandoval-Schaefer,Kristyn Carter,Diane E King,Henry C Hsia,Michael I Gazes,Steven D Vyce,Ryan R Driskell,Kathryn Miller-Jensen,Valerie HorsleyPubMed 38127424
- Cell 186:5910-5924.e172023A tridimensional atlas of the developing human head.Applications:IHC - WmtReactive species:HumanRaphael Blain,Gérard Couly,Eimad Shotar,Joséphine Blévinal,Maryne Toupin,Anais Favre,Ali Abjaghou,Megumi Inoue,Edwin Hernández-Garzón,Frédéric Clarençon,Frédéric Chalmel,Séverine Mazaud-Guittot,Paolo Giacobini,Yorick Gitton,Alain ChédotalPubMed 38070509
- Nature communications 14:81632023Generation of three-dimensional meat-like tissue from stable pig epiblast stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaoxiang Zhu,Dengfeng Gao,Linzi Li,Yixuan Yao,Yingjie Wang,Minglei Zhi,Jinying Zhang,Xinze Chen,Qianqian Zhu,Jie Gao,Tianzhi Chen,Xiaowei Zhang,Tong Wang,Suying Cao,Aijin Ma,Xianchao Feng,Jianyong HanPubMed 38071210
- Nature communications 14:80692023Systemically administered wound-homing peptide accelerates wound healing by modulating syndecan-4 function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHoracio Maldonado,Bryan D Savage,Harlan R Barker,Ulrike May,Maria Vähätupa,Rahul K Badiani,Katarzyna I Wolanska,Craig M J Turner,Toini Pemmari,Tuomo Ketomäki,Stuart Prince,Martin J Humphries,Erkki Ruoslahti,Mark R Morgan,Tero A H JärvinenPubMed 38057316
- Cell transplantation 32:96368972312152332023Isolation, Expansion, and Endoscopic Delivery of Autologous Enteric Neuronal Stem Cells in Swine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyo Hotta,Weikang Pan,Sukhada Bhave,Nandor Nagy,Rhian Stavely,Takahiro Ohkura,Kumar Krishnan,Geoffrey de Couto,Richard Myers,Luis Rodriguez-Borlado,Alan J Burns,Allan M GoldsteinPubMed 38049927
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 99.95% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ACTA2. The exact immunogen used to generate this antibody is proprietary information. Database link P62736
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-2 µg/mL | Notes - |
Species Mouse | Dilution info 0.5-2 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 - 1/200 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/50 - 1/200 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
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Target data
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Alternative names
ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
Recommended products
Anti-alpha smooth muscle Actin antibody (ab5694) is a rabbit polyclonal antibody detecting alpha smooth muscle Actin in Western Blot, IHC-P. Suitable for Human, Mouse.
- Over 3000 publications
- Trusted since 2003
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 99.95% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ACTA2. The exact immunogen used to generate this antibody is proprietary information. Database link P62736
- Purification technique
- Affinity purification Immunogen
- Specificity
Alpha smooth muscle actin antibody (ab5694) stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. ab5694 reacts with tumors arising from smooth muscles and myoepithelial cells.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-alpha smooth muscle Actin antibody (ab5694) is a rabbit polyclonal antibody and is validated for use in IHC-P and WB.
Anti-alpha smooth muscle Actin antibody (ab5694) was first used in a scientific publication in 1985 and has been cited over 3004 times in peer reviewed journals. It's performance in Western blot, immunofluorescence and IHC in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-alpha smooth muscle Actin antibody (ab5694) has high sensitivity and specificity.
Anti-alpha smooth muscle Actin antibody (ab5694) has 150 independent reviews from customers.
Anti-alpha smooth muscle Actin antibody (ab5694) specifically detects alpha smooth muscle Actin (UniProt ID: P62736; Molecular weight: 42kDa) and is sold in 100 µg selling sizes.
Alpha-Smooth Muscle Actin (α-SMA) is a critical protein involved in smooth muscle contraction, encoded by the ACTA2 gene. Predominantly found in vascular smooth muscle cells, α-SMA plays a vital role in fibrogenesis and wound healing. It is often used as a marker for myofibroblast formation, which are specialized cells that contribute to tissue repair and fibrosis. The expression of α-SMA is associated with the activation of myofibroblasts, making it an important indicator in studies of fibrosis and other related conditions.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.
- Biological function summary
Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.
- Pathways
Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.
- Associated diseases and disorders
Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
This image is courtesy of a customer review submitted by Cameron Johnstone.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of 10% formalin-fixed Human transitional cell carcinoma of the kidney. Stained with ab5694 at 1/150 dilution. Secondary antibody used was goat anti-rabbit Alexa-568® at 1/500 dilution. Blocking was done with 5% serum for 1 hour at 25°C. The sample was incubated with the primary antibody for 1hour at 25°C with 5% normal goat serum. Antigen retrieval method was heat mediated with EDTA pH 8.
Image from Lee H. et al., BMC Dev Biol. 2014 Dec 21;14:48. Fig 2Bdoi: 10.1186/s12861-014-0048-3 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
Pancreatic vessel imaging in the intact adult mouse pancreas. In adult mouse tissues (12 weeks old), imaging was performed after CLARITY. Three-dimensional projection clarified mouse pancreas with capillary immunostained for α-smooth muscle actin (green). Scale bar, 200 μm.From a study, that aimed to improve the original CLARITY procedure and developed specific CLARITY protocols for various intact organs.
Western blot - Anti-alpha smooth muscle Actin antibody (ab5694)
Incubated with the primary antibody at 4°C overnight.
Incubated with the secondary antibody at room temperature for 1 hour.
Lanes 1 - 3: Western blot - Anti-alpha smooth muscle Actin antibody (ab5694) at 1 µg/mL
Lanes 4 - 5: Western blot - Anti-alpha smooth muscle Actin antibody (ab5694)
Lane 1: HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate - overexpressing alpha-Actin at 20 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) cell lysate at 20 µg
Lanes 3 and 5: Mouse heart tissue homogenate at 20 µg
Lane 4: NIH/3T3 cell lysate at 20 µg
SecondaryAll lanes: Fluor 750-conjugated goat anti-rabbit IgG (H+L) at 1/12500 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Image from Lloyd-Lewis B. et al., Breast Cancer Res. 2016 18: 127. Fig 2.; doi: 10.1186/s13058-016-0754-9. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
Passive CLARITY technique (PACT)-sorbitol refractive index matching solution (sRIMS) clearing and 3D imaging of virgin and lactating mouse mammary tissue. a PACT-sRIMS tissue clearing and immunostaining protocol and timeline. Three-dimensional confocal imaging of PACT-sRIMS-cleared virgin (b) and lactating (c) mammary glands immunostained with basal cell markers (K5 and smooth muscle actin (stained with ab5694)) and luminal cell markers (K8 and E-cadherin (E-CAD)). Main image shows the maximum intensity projection of the entire image sequence, with thin optical slices (1 μm) and their depth (z value) relative to the first image in the image sequence.
From a paper comparing imaging of intact virgin and lactating mammary glands using 3D imaging of solvent-cleared organs, see deep brain (seeDB), clear unobstructed brain imaging cocktails (CUBIC) and passive clarity technique.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
This picture shows formalin-fixed, paraffin embedded mouse intestine and mesentery, the optimal dilution is 1:1600 to 1:3200, incubation overnight at 4oC, counterstained with Hematoxylin.
This image was kindly supplied as part of the review by JQ Zhang.
Freytsis, M. et al PLoS One. 2018 Jan 5;13(1):e0190623. doi: 10.1371/journal.pone.0190623. eCollection 2018 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
Representative histology of aortic valve leaflets from aged mice demonstrates changes in pRb cKO AoVA) Masson's trichrome showing reduced collagen staining (blue) in leaflet from pRb cKO mouse with aortic regurgitation (AR). B) Movat pentachrome showing more diffuse collage staining (yellow) in fibrosa, but normal proteoglycan staining (blue) in the spongiosa layer of the leaflet from pRb cKO with AR. C) Immunohistochemistry for α-SMA, demonstrating presence of activated myofibroblasts throughout leaflets of pRb cKO mouse with and without AR. Scale bar is 50μm.
Alpha smooth muscle Actin is detected witn ab5694 at 1/1000 dilution.
(From Figure 2 of Freytsis et al)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody (ab5694)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling alpha smooth muscle Actin with ab5694 at a dilution of 1/1000. Heat mediated antigen retrieval was performed for 35 minutes followed by cooling for 20 minutes. Sections were incubated with the primary antibody for 1 hour followed by incubation with a biotinylated secondary antibody (Goat Anti-Rabbit IgG H&L (Biotin) ab6720) for 30 minutes then HRP-Streptavidin for 30 minutes. Developed using DAB chromogen substrate (5-10 minutes). Counter stained with hematoxylin.
Magnification: left - 10X, right - 40X.
Western blot - Anti-alpha smooth muscle Actin antibody (ab5694)
Please note that ab5694 does not appear to be specific to smooth muscle.
All lanes: Western blot - Anti-alpha smooth muscle Actin antibody (ab5694) at 1 µg/mL
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear cell lysate at 20 µg
Lane 2: HeLa whole cell lysate at 20 µg
Lane 3: A431 (Human epidermoid carcinoma cell line) cell lysate at 20 µg
Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 20 µg
Lane 5: HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg
SecondaryAll lanes: Alexa Fluor anti-rabbit at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 30 kDa, 35 kDa, 37 kDa, 42 kDa, 50 kDa, 75 kDa
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