AB220179

Anti-alpha smooth muscle Actin antibody [ACTA2/791]

Name: Anti-alpha smooth muscle Actin antibody [ACTA2/791] (ab220179) | Abcam
Brand: Abcam Limited
SKU: AB220179
Price: 495 USD
Availability: InStock

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(9 Publications)

Mouse Monoclonal alpha smooth muscle Actin antibody. Suitable for ICC, IHC-P and reacts with Human, Rat samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ACTA2.

View Alternative Names

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

5 Images

Unknown

Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (AB220179)

Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with ab220179 followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (AB220179)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (AB220179)

Immunohistochemical analysis of formalin fixed paraffin embedded Human colon carcinoma tissue labeling alpha smooth muscle Actin with ab220179 at 0.5 μg/ml.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (AB220179)

Immunohistochemical analysis of formalin fixed paraffin embedded Human leiomyosarcoma tissue labeling alpha smooth muscle Actin with ab220179 at 0.5 μg/ml.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (AB220179)

Immunohistochemical analysis of formalin fixed paraffin embedded Human angiosarcoma tissue labeling alpha smooth muscle Actin with ab220179 at 0.5 μg/ml.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (AB220179)

Immunohistochemical analysis of formalin fixed paraffin embedded Rat lung tissue labeling alpha smooth muscle Actin with ab220179 at 0.5 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ACTA2/791

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Rat, Human

Applications

ICC, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ACTA2.

P62736

Specificity

Actin proteins have very high homology. The immunogen for this product is based on the ACTA2 protein. There is a chance that this product will detect other actin family members. The cross reactivity has not been experimentally determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.25-0.5 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.25-0.5 µg/mL", "IHCP-species-notes": "" }, "Chicken": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

ab220179 was purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.

Biological function summary

Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.

Pathways

Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.

Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Biomaterials science 13:6368-6377 PubMed41044926

2025

A tissue-engineered endothelial cell - smooth muscle cell arteriole-like model.

Applications

Unspecified application

Species

Unspecified reactive species

Ninghao Zhu,Lily Liang,Nan Zhao,Haosong Chen,Peter C Searson

Scientific reports 15:26596 PubMed40695950

2025

The new perspective on understanding the mechanisms of cardiovascular diseases development.

Applications

Unspecified application

Species

Unspecified reactive species

Ulyana Khovantseva,Diana Kiseleva,Vadim Cherednichenko,Deyyara Chakal,Denis Breshenkov,Yuliya Markina,Rustam Ziganshin,Eduard Charchyan,Alexander Markin

Cells 13: PubMed39594574

2024

Cancer-Associated Fibroblast-Derived FGF7 Promotes Clear Cell Renal Cell Carcinoma Progression and Macrophage Infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Man Jia,Mingyu Xie,Xixi Luo,Huiping Wang,Chunyan Duan,Wanni Lai,Rongyang Dai,Ronghao Wang

International journal of oral science 15:38 PubMed37679344

2023

Single-cell transcriptomic analysis uncovers the origin and intratumoral heterogeneity of parotid pleomorphic adenoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuyun Xu,Jiaxiang Xie,Rongsong Ling,Shengqi Ouyang,Gan Xiong,Yanwen Lu,Bokai Yun,Ming Zhang,Wenjin Wang,Xiqiang Liu,Demeng Chen,Cheng Wang

The Journal of clinical investigation 133: PubMed36602864

2023

A systems biology approach identifies the role of dysregulated PRDM6 in the development of hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Kushan L Gunawardhana,Lingjuan Hong,Trojan Rugira,Severin Uebbing,Joanna Kucharczak,Sameet Mehta,Dineth R Karunamuni,Brenda Cabera-Mendoza,Neeru Gandotra,Curt Scharfe,Renato Polimanti,James P Noonan,Arya Mani

Cancer gene therapy 30:149-162 PubMed36123390

2022

YAP 5-methylcytosine modification increases its mRNA stability and promotes the transcription of exosome secretion-related genes in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Yu,Congcong Zhang,Yikun Wang,Xiaoting Tian,Yayou Miao,Fanyu Meng,Lifang Ma,Xiao Zhang,Jinjing Xia

Frontiers in cardiovascular medicine 9:886687 PubMed35665251

2022

Microvascular Dysfunction in Skeletal Muscle Precedes Myocardial Vascular Changes in Diabetic Cardiomyopathy: Sex-Dependent Differences.

Applications

Unspecified application

Species

Unspecified reactive species

Sadi Loai,Xuetao Sun,Mansoor Husain,Michael A Laflamme,Herman Yeger,Sara S Nunes,Hai-Ling Margaret Cheng

Frontiers in physiology 12:714785 PubMed34408668

2021

Upregulation of Calcium Homeostasis Modulators in Contractile-To-Proliferative Phenotypical Transition of Pulmonary Arterial Smooth Muscle Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marisela Rodriguez,Jiyuan Chen,Pritesh P Jain,Aleksandra Babicheva,Mingmei Xiong,Jifeng Li,Ning Lai,Tengteng Zhao,Moises Hernandez,Angela Balistrieri,Sophia Parmisano,Tatum Simonson,Ellen Breen,Daniela Valdez-Jasso,Patricia A Thistlethwaite,John Y-J Shyy,Jian Wang,Joe G N Garcia,Ayako Makino,Jason X-J Yuan

Cell reports 32:108172 PubMed32997990

2020

Actin R256 Mono-methylation Is a Conserved Post-translational Modification Involved in Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Ashok Kumar,Yuan Zhong,Amelie Albrecht,Pau Biak Sang,Adrian Maples,Zhenan Liu,Vinesh Vinayachandran,Rohit Reja,Chia-Fang Lee,Ashutosh Kumar,Jiyuan Chen,Jing Xiao,Bongsoo Park,Jianjun Shen,Bin Liu,Maria D Person,Kathleen M Trybus,Kam Y J Zhang,B Franklin Pugh,Kristine E Kamm,Dianna M Milewicz,Xuetong Shen,Prabodh Kapoor

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha smooth muscle Actin antibody [ACTA2/791]

Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (AB220179)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (AB220179)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (AB220179)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (AB220179)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (AB220179)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise