Mouse Monoclonal alpha smooth muscle Actin antibody. Carrier free. Suitable for IHC-P and reacts with Rat, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ACTA2.
IgG2a
Mouse
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IHC-P | |
---|---|
Human | Tested |
Mouse | Predicted |
Rat | Tested |
Chicken | Predicted |
Cow | Predicted |
Rabbit | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 0.25000-0.50000 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 0.25000-0.50000 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rabbit, Chicken, Cow | Dilution info - | Notes - |
Select an associated product type
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
Mouse Monoclonal alpha smooth muscle Actin antibody. Carrier free. Suitable for IHC-P and reacts with Rat, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ACTA2.
IgG2a
Mouse
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
ACTA2/791
Affinity purification Protein A/G
Actin proteins have very high homology. The immunogen for this product is based on the ACTA2 protein. There is a chance that this product will detect other actin family members. The cross reactivity has not been experimentally determined.
kappa
ab22179 was purified from Bioreactor Concentrate by Protein A/G.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
ab212795 is a carrier free version of Anti-alpha smooth muscle Actin antibody [ACTA2/791] ab220179.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.
Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.
Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.
Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Immunohistochemical analysis of formalin fixed, paraffin embedded Human angiosarcoma tissue labeling alpha smooth muscle Actin with Anti-alpha smooth muscle Actin antibody [ACTA2/791] ab220179 at 0.5 µg/ml.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-alpha smooth muscle Actin antibody [ACTA2/791] ab220179)
.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human leiomyosarcoma tissue labeling alpha smooth muscle Actin with Anti-alpha smooth muscle Actin antibody [ACTA2/791] ab220179 at 0.5 µg/ml.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-alpha smooth muscle Actin antibody [ACTA2/791] ab220179)
.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon carcinoma tissue labeling alpha smooth muscle Actin with Anti-alpha smooth muscle Actin antibody [ACTA2/791] ab220179 at 0.5 µg/ml.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-alpha smooth muscle Actin antibody [ACTA2/791] ab220179)
.
Immunohistochemical analysis of formalin fixed, paraffin embedded Rat lung tissue labeling alpha smooth muscle Actin with Anti-alpha smooth muscle Actin antibody [ACTA2/791] ab220179 at 0.5 µg/ml.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-alpha smooth muscle Actin antibody [ACTA2/791] ab220179)
.
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