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Rabbit Recombinant Monoclonal alpha smooth muscle Actin antibody. Suitable for IHC-P, ICC, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ACTA2 aa 1-50.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [BLR082G] (AB265588), expandable thumbnail
  • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [BLR082G] (AB265588), expandable thumbnail
  • Western blot - Anti-alpha smooth muscle Actin antibody [BLR082G] (AB265588), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [BLR082G] (AB265588), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human ACTA2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P62736

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICCWB
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/500.00000

Notes

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal alpha smooth muscle Actin antibody. Suitable for IHC-P, ICC, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ACTA2 aa 1-50.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human ACTA2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P62736
Clone number

BLR082G

Purification technique

Affinity purification

Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.

Biological function summary

Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.

Pathways

Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.

Associated diseases and disorders

Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [BLR082G] (ab265588), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [BLR082G] (ab265588)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.

  • Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [BLR082G] (ab265588), expandable thumbnail

    Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [BLR082G] (ab265588)

    Immunocytochemical analysis of formalin-fixed, paraffin-embedded IMR-90 cells labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.

  • Western blot - Anti-alpha smooth muscle Actin antibody [BLR082G] (ab265588), expandable thumbnail

    Western blot - Anti-alpha smooth muscle Actin antibody [BLR082G] (ab265588)

    Cells prepared using NETN lysis buffer.

    All lanes: Western blot - Anti-alpha smooth muscle Actin antibody [BLR082G] (ab265588) at 1/1000 dilution

    Lane 1: MCF7 whole cell lysate at 50 µg

    Lane 2: A549 whole cell lysate at 50 µg

    Lane 3: Jurkat whole cell lysate at 50 µg

    Lane 4: IMR-90 whole cell lysate at 10 µg

    Lane 5: HepG2 whole cell lysate at 50 µg

    Lane 6: LNCaP whole cell lysate at 50 µg

    Lane 7: RKO whole cell lysate at 50 µg

    Lane 8: K562 whole cell lysate at 50 µg

    Lane 9: NIH/3T3 whole cell lysate at 50 µg

    Lane 10: TCMK whole cell lysate at 50 µg

    Predicted band size: 42 kDa

    Exposure time: 30s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [BLR082G] (ab265588), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [BLR082G] (ab265588)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast tissue labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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