Rabbit Recombinant Monoclonal alpha smooth muscle Actin antibody. Suitable for IHC-P, ICC, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ACTA2 aa 1-50.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Liquid
Monoclonal
IHC-P | ICC | WB | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
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Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
Rabbit Recombinant Monoclonal alpha smooth muscle Actin antibody. Suitable for IHC-P, ICC, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ACTA2 aa 1-50.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Liquid
Monoclonal
BLR082G
Affinity purification
Purified from TCS.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This supplementary information is collated from multiple sources and compiled automatically.
Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.
Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.
Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.
Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.
Immunocytochemical analysis of formalin-fixed, paraffin-embedded IMR-90 cells labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.
Cells prepared using NETN lysis buffer.
All lanes: Western blot - Anti-alpha smooth muscle Actin antibody [BLR082G] (ab265588) at 1/1000 dilution
Lane 1: MCF7 whole cell lysate at 50 µg
Lane 2: A549 whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Lane 4: IMR-90 whole cell lysate at 10 µg
Lane 5: HepG2 whole cell lysate at 50 µg
Lane 6: LNCaP whole cell lysate at 50 µg
Lane 7: RKO whole cell lysate at 50 µg
Lane 8: K562 whole cell lysate at 50 µg
Lane 9: NIH/3T3 whole cell lysate at 50 µg
Lane 10: TCMK whole cell lysate at 50 µg
Predicted band size: 42 kDa
Exposure time: 30s
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast tissue labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection: DAB.
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