AB265588

Anti-alpha smooth muscle Actin antibody [BLR082G]

Recombinant
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(8 Publications)

Rabbit Recombinant Monoclonal alpha smooth muscle Actin antibody. Suitable for IHC-P, ICC, WB and reacts with Human, Mouse samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human ACTA2 aa 1-50.

View Alternative Names

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [BLR082G] (AB265588)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [BLR082G] (AB265588)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection : DAB.

Supplier Data

Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [BLR082G] (AB265588)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded IMR-90 cells labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection : DAB.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [BLR082G] (AB265588)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast tissue labeling alpha smooth muscle Actin with ab265588 at 1/100 dilution. Detection : DAB.

Supplier Data

Western blot - Anti-alpha smooth muscle Actin antibody [BLR082G] (AB265588)

Cells prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-alpha smooth muscle Actin antibody [BLR082G] (ab265588) at 1/1000 dilution

Lane 1:

MCF7 whole cell lysate at 50 µg

Lane 2:

A549 whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

IMR-90 whole cell lysate at 10 µg

Lane 5:

HepG2 whole cell lysate at 50 µg

Lane 6:

LNCaP whole cell lysate at 50 µg

Lane 7:

RKO whole cell lysate at 50 µg

Lane 8:

K562 whole cell lysate at 50 µg

Lane 9:

NIH/3T3 whole cell lysate at 50 µg

Lane 10:

TCMK whole cell lysate at 50 µg

Predicted band size: 42 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR082G

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC, WB

applications

Immunogen

Synthetic Peptide within Human ACTA2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P62736

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from TCS.

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.

Biological function summary

Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.

Pathways

Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.

Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA2

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Surgery open science 20:106-115 PubMed39021615

2024

Development and validation of a mouse model to investigate post surgical pain after laparotomy.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Martinez,Thomas Maisey,Nicola Ingram,Nikil Kapur,Paul A Beales,David G Jayne

Experimental and therapeutic medicine 23:420 PubMed35601076

2022

Simvastatin alleviates epithelial-mesenchymal transition and oxidative stress of high glucose-induced lens epithelial cells by inhibiting RhoA/ROCK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jianming Fu,Xiaojie Hu

International journal of ophthalmology 14:1674-1682 PubMed34804856

2021

TGF-β2-induced NEAT1 regulates lens epithelial cell proliferation, migration and EMT by the miR-26a-5p/FANCE axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Hui Yu,Shao-Yi Liu,Cheng-Fang Li

Experimental and therapeutic medicine 22:1237 PubMed34539833

2021

Long non-coding RNA miR155HG silencing restrains ovarian cancer progression by targeting the microRNA-155-5p/tyrosinase-related protein 1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Aiping Wen,Le Luo,Chengchao Du,Xin Luo

International journal of molecular sciences 22: PubMed34445305

2021

Anti-Inflammatory Effect of Allicin Associated with Fibrosis in Pulmonary Arterial Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

José L Sánchez-Gloria,Constanza Estefanía Martínez-Olivares,Pedro Rojas-Morales,Rogelio Hernández-Pando,Roxana Carbó,Ivan Rubio-Gayosso,Abraham S Arellano-Buendía,Karla M Rada,Fausto Sánchez-Muñoz,Horacio Osorio-Alonso

Antioxidants (Basel, Switzerland) 9: PubMed33203103

2020

Effects of Allicin on Pathophysiological Mechanisms during the Progression of Nephropathy Associated to Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Abraham Said Arellano-Buendía,Luis Gerardo Castañeda-Lara,María L Loredo-Mendoza,Fernando E García-Arroyo,Pedro Rojas-Morales,Raúl Argüello-García,Juan G Juárez-Rojas,Edilia Tapia,José Pedraza-Chaverri,Laura Gabriela Sánchez-Lozada,Horacio Osorio-Alonso

Stem cell research & therapy 11:362 PubMed32811535

2020

SIRT1-modified human umbilical cord mesenchymal stem cells ameliorate experimental peritoneal fibrosis by inhibiting the TGF-β/Smad3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhong Guo,Liuwei Wang,Rong Gou,Yulin Wang,Xiujie Shi,Xinxin Pang,Lin Tang

Biochemical and biophysical research communication : PubMed32085895

2020

LINC00028 regulates the development of TGFβ1-treated human tenon capsule fibroblasts by targeting miR-204-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Huali Sui,Shanshan Fan,Wenjing Liu,Yingchao Li,Xuan Zhang,Yunhong Du,Huijing Bao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha smooth muscle Actin antibody [BLR082G]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [BLR082G] (AB265588)

Immunocytochemistry - Anti-alpha smooth muscle Actin antibody [BLR082G] (AB265588)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [BLR082G] (AB265588)

Western blot - Anti-alpha smooth muscle Actin antibody [BLR082G] (AB265588)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise