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AB320059

Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free

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Goat Recombinant Monoclonal alpha smooth muscle Actin antibody. Carrier free. Suitable for ICC/IF, IHC-P and reacts with Human, Rat, Mouse samples.

View Alternative Names

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free (AB320059)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free (AB320059)

ab320059 staining ACTA2 in HeLa (positive) and A-431 (negative) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal donkey serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab320059 at 1µg/ml (shown in green) and ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with ab150133 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free (AB320059)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free (AB320059)

Immunofluorescence staining of ACTA2 in sections of formalin-fixed paraffin-embedded human colon (positive) and human brain (negative)*. Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab320059 at 1/500 dilution and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free (AB320059)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free (AB320059)

Immunofluorescence staining of ACTA2 in sections of formalin-fixed paraffin-embedded mouse stomach (positive) and mouse brain (negative).Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab320059 at 1/500 dilution and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions antibody concentrations and incubation times.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free (AB320059)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free (AB320059)

Immunofluorescence staining of ACTA2 in sections of formalin-fixed paraffin-embedded rat stomach (positive) and rat brain (negative).Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab320059 at 1/500 dilution and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions antibody concentrations and incubation times.

Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free (AB320059)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free (AB320059)

ab320059 staining ACTA2 in SV40LT-SMC cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal donkey serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab320059 at 1µg/ml (shown in green) and ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with ab150133 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

  • Unconjugated

    Anti-alpha smooth muscle Actin antibody [EPR5368]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-alpha smooth muscle Actin antibody [EPR5368]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [EPR5368]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [EPR5368]

  • 578 PE

    PE Anti-alpha smooth muscle Actin antibody [EPR5368]

  • Carrier free

    Anti-alpha smooth muscle Actin antibody [EPR5368] - BSA and Azide free

  • 660 APC

    APC Anti-alpha smooth muscle Actin antibody [EPR5368]

  • Unconjugated

    Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric)

  • Unconjugated

    Anti-alpha smooth muscle Actin [EPR5368] – Chicken IgY (Chimeric)

Key facts

Host species

Goat

Clonality

Monoclonal

Clone number

EPR5368

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Rat, Mouse

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab320059 is the PBS only version of ab320058.

This goat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab124964). By design, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.
Biological function summary

Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.

Pathways

Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.

Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
See full target information ACTA2

Additional targets

Acta2,Acta2
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Product promise

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For full details, please see our Terms & Conditions

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