AB150301

Anti-alpha smooth muscle Actin antibody [SP171]

BOND RX™ Validated
20ul selling size
RabMAb
Advanced Validation
Recombinant
KO Validated
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(31 Publications)

Anti-alpha smooth muscle Actin antibody [SP171] (ab150301) is a rabbit monoclonal antibody detecting alpha smooth muscle Actin in Flow Cytometry (Intra), IHC-P, ICC/IF, mIHC. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

11 Images

Multiplex immunohistochemistry - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Fluorescence multiplex immunohistochemical analysis of human liver tissue (formalin-fixed paraffin-embedded section). Panel A shows merged staining of ab195872 anti-Cytokeratin 19 stained on branch of bile ducts (magenta; Opal™690) at 1 : 8000 ( 0.127 μg/ml) [Panel B], ab275376 anti-Factor VIII stained on endothelial cells (red; Opal™570) at 1 : 1000 ( 0.457 μg/ml) [Panel C], and ab150301 anti-alpha smooth muscle Actin stained on smooth muscles (green; Opal™520) at 1 : 200 ( 0.14 μg/ml) [Panel C] on human liver. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining : in the order of ab195872 for 30 mins, ab275376 for 30 mins and ab150301 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon tissue labelling alpha smooth muscle Actin with ab150301 at 1/200 dilution.

Flow Cytometry (Intracellular) - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling alpha smooth muscle Actin with purified ab150301 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling alpha smooth muscle Actin with ab150301 at 1/200 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

ab150301 staining alpha smooth muscle Actin in wild-type HeLa cells (top panel) and ACTA2 knockout HeLa cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab150301 at 5μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor^® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor^® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling alpha smooth muscle Actin with ab150301 at 1/200 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling alpha smooth muscle Actin with ab150301 at 1/200 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Flow Cytometry analysis of C6 (Rat glial tumor glial cell) cells labeling alpha smooth muscle Actin with purified ab150301 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling alpha smooth muscle Actin with purified ab150301. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Flow Cytometry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling alpha smooth muscle Actin with purified ab150301 at 1/200 dilution (0.825 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

ab150301 staining alpha smooth muscle Actin in SV40LT-SMC cells (positive control, top panel) and A431 cells (negative control, bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab150301 at 1μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor^® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor^® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Carrier free

Anti-alpha smooth muscle Actin antibody [SP171] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [SP171]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-alpha smooth muscle Actin antibody [SP171]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [SP171]
660 APC

APC Anti-alpha smooth muscle Actin antibody [SP171]
578 PE

PE Anti-alpha smooth muscle Actin antibody [SP171]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-alpha smooth muscle Actin antibody [SP171]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-alpha smooth muscle Actin antibody [SP171]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-alpha smooth muscle Actin antibody [SP171]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP171

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), IHC-P, ICC/IF, mIHC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/200", "mIHC-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 mg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "Compared with ab150301, <a href='/en-us/products/primary-antibodies/alpha-smooth-muscle-actin-antibody-epr5368-ab124964'>ab124964</a> has higher sensitivity, we recommend <a href='/en-us/products/primary-antibodies/alpha-smooth-muscle-actin-antibody-epr5368-ab124964'>ab124964</a> as an alternative for testing alpha smooth muscle Actin in western blot.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "" }, "Mouse": { "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "" }, "Rat": { "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 mg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "" }, "Chicken": { "mIHC-species-checked": "predicted", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "mIHC-species-checked": "predicted", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "mIHC-species-checked": "predicted", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "mIHC-species-checked": "predicted", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-alpha smooth muscle Actin antibody [SP171] (ab150301) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in Human, Mouse, Rat samples.

Collaboration
Anti-alpha smooth muscle Actin [SP171] (ab150301) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Trusted by the scientific community
Anti-alpha smooth muscle Actin [SP171] (ab150301) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-alpha smooth muscle Actin antibody [SP171] (ab150301) has been confirmed by Western blot testing in ACTA2 Knockout HeLa cells.

Other related products
We have a range of other formats of antibody clone [SP171] also available for your convenience: ab150301, Carrier free - ab242395, Alexa Fluor^® 488 - ab267536, Alexa Fluor^® 647 - ab267537, ab279419, APC - ab310827, PE - ab310903, Alexa Fluor^® 594 - ab311730, Alexa Fluor^® 568 - ab313007, Alexa Fluor^® 555 - ab313211, Alexa Fluor^® 750 - ab321123

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.

Biological function summary

Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.

Pathways

Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.

Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA2

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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Bioengineering & translational medicine 10:e70025 PubMed41030280

2025

Engineering biomimetic nanovesicles for PEBP1 mRNA delivery to inhibit ferroptosis in abdominal aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Lulu Chen,Bicheng Chen,Xiang Su

Advanced biology 9:e2400437 PubMed39960128

2025

The Role of Luteolin in Inhibiting Prostaglandin-Endoperoxide Synthase 2 to Relieve Neointimal Hyperplasia in Arteriovenous Fistula.

Applications

Unspecified application

Species

Unspecified reactive species

Ruibin Zhang,Wei Li,Jihua Yang,Xiujie Fan,Huili Fan,Wei Li

Investigative ophthalmology & visual science 65:25 PubMed39661356

2024

Epiretinal Membrane: Correlations Among Clinical, Immunohistochemical, and Biochemical Features and Their Prognostic Implications.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Seung Yang,Suho Choi,Soojin Kim,Chan Hong Min,Dongwoo Kim,Youngseop Lee,Minji Kim,Donghoon Koo,Jaiyoung Ryu,Jeongmin Kim

The Tohoku journal of experimental medicine : PubMed39603602

2024

Yangfei Tongluo Decoction Alleviated Fibrosis and Inflammation in Post-Infectious Bronchiolitis Obliterans via Hedgehog Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxia Chen,Libiao Wang,Sujing Su,Shuhua Fan

Cells 13: PubMed39404393

2024

Role of WISP1 in Stellate Cell Migration and Liver Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela González,Gisela Campos,Larissa Pütter,Adrian Friebel,Christian H Holland,Leonhard Holländer,Ahmed Ghallab,Zaynab Hobloss,Maiju Myllys,Stefan Hoehme,Nadja M Meindl-Beinker,Steven Dooley,Rosemarie Marchan,Thomas S Weiss,Jan G Hengstler,Patricio Godoy

Cell reports. Medicine 5:101731 PubMed39303711

2024

Reprograming immunosuppressive microenvironment by eIF4G1 targeting to eradicate pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lihong He,Xiaozhen Zhang,Fukang Shi,Hanjia Zhang,Yan Chen,Kang Sun,Hanshen Yang,Jiatao Shi,Zihao Lin,Qingsong Lu,Sicheng Wang,Linyue Liu,Xinyuan Liu,Qingbo Meng,Junmin Huang,Pinglong Xu,Xueli Bai,Tingbo Liang

Biophotonics discovery 1: PubMed39011049

2024

Quantifying collagen reorganization during immunotherapy in murine melanoma with second harmonic generation imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Alexa R Heaton,Nathaniel J Burkard,Paul M Sondel,Melissa C Skala

Respiratory research 25:270 PubMed38987833

2024

LncRNAH19 acts as a ceRNA of let-7 g to facilitate endothelial-to-mesenchymal transition in hypoxic pulmonary hypertension via regulating TGF-β signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yu,Jiabing Huang,Xu Liu,Juan Li,Miao Yu,Minghui Li,Yuliang Xie,Ye Li,Junyu Qiu,Zhou Xu,Tiantian Zhu,Weifang Zhang

Kidney & blood pressure research 49:385-396 PubMed38735279

2024

Indoxyl Sulfate Aggravates Podocyte Damage through the TGF-β1/Smad/ROS Signalling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Jia,Lihua Lin,Kang Xun,Damei Li,Weijiang Wu,Shaobo Sun,Hong Qiu,Donghua Jin

Investigative ophthalmology & visual science 65:46 PubMed38687491

2024

Single-Nuclei Characterization of Lacrimal Gland in Scopolamine-Induced Dry Eye Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Tang,Shengqian Dou,Chao Wei,Ziwen Sun,Di Sun,Qingjun Zhou,Lixin Xie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha smooth muscle Actin antibody [SP171]

Multiplex immunohistochemistry - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Flow Cytometry (Intracellular) - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Flow Cytometry (Intracellular) - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Flow Cytometry (Intracellular) - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [SP171] (AB150301)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (31)

Product promise