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AB182781

Anti-Alpha SNAP antibody

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(1 Publication)

Rabbit Polyclonal Alpha SNAP antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NAPA aa 1 to C-terminus.

View Alternative Names

SNAPA, NAPA, Alpha-soluble NSF attachment protein, SNAP-alpha, N-ethylmaleimide-sensitive factor attachment protein alpha

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody (AB182781)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody (AB182781)

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling NAPA with ab182781 at 1/25 dilution. The image on the right was treated with the immunogen.

Western blot - Anti-Alpha SNAP antibody (AB182781)
  • WB

Supplier Data

Western blot - Anti-Alpha SNAP antibody (AB182781)

All lanes:

Western blot - Anti-Alpha SNAP antibody (ab182781) at 1/350 dilution

Lane 1:

HeLa cell lysate at 40 µg

Lane 2:

Jurkat cell lysate at 40 µg

Lane 3:

Human liver cancer tissue lysate at 40 µg

Lane 4:

Mouse brain tissue lysate at 40 µg

Secondary

All lanes:

Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

Predicted band size: 33 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NAPA aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P54920

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Proteins & Peptides

AB100963

Recombinant Human Alpha SNAP protein (His tag N-Terminus)

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha SNAP also known as NSF attachment protein alpha is an important component of cellular machinery. This protein weighs approximately 33 kDa and facilitates membrane trafficking processes. It is expressed ubiquitously meaning it is found throughout various tissues in the body. Alpha SNAP is essential for the proper function of membrane fusion events playing a significant role in exocytosis and endocytosis.
Biological function summary

Alpha SNAP works as a part of the SNARE complex. It acts as a mediator that helps recycle SNARE proteins which are key components in vesicle fusion processes. The protein binds to the SNARE complex allowing NSF (N-ethylmaleimide-sensitive factor) to disassemble it enabling the components to be reused. This recycling is critical for continuous vesicular transport aiding in the maintenance of cellular function.

Pathways

Alpha SNAP functions mainly in the vesicular transport and membrane trafficking pathways. It interacts with NSF and other SNARE proteins to facilitate these processes. Within the cellular transport pathway Alpha SNAP ensures efficient neurotransmitter release and hormone secretion. It is involved in the endocytic and exocytic pathways both vital for proper cellular communication and function.

Mutations or dysfunctions in Alpha SNAP have been linked to neurological disorders such as epilepsy due to its role in neurotransmitter release. Its malfunction can disrupt synaptic communication leading to abnormal neural activity. Additionally Alpha SNAP may be implicated in some types of neurodegenerative diseases interacting with proteins such as NSF which are important for synaptic maintenance and plasticity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus (Probable). Together with GNA12 promotes CDH5 localization to plasma membrane (PubMed : 15980433).
See full target information NAPA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in aging neuroscience 15:1282855 PubMed38035268

2023

Protection against Aβ-induced neuronal damage by KU-32: PDHK1 inhibition as important target.

Applications

Unspecified application

Species

Unspecified reactive species

Ranu Pal,Dongwei Hui,Heather Menchen,Huiping Zhao,Olivier Mozziconacci,Heather Wilkins,Brian S J Blagg,Christian Schöneich,Russell H Swerdlow,Mary L Michaelis,Elias K Michaelis
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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