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AB248607

Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Alpha SNAP antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SNAPA, NAPA, Alpha-soluble NSF attachment protein, SNAP-alpha, N-ethylmaleimide-sensitive factor attachment protein alpha

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (AB248607)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (AB248607)

This data was developed using ab133673, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Alpha SNAP with ab133673 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (AB248607)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (AB248607)

This data was developed using ab133673, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HepG2 cells labelling Alpha SNAP with ab133673 at 1/100.

Western blot - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (AB248607)
  • WB

Unknown

Western blot - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (AB248607)

This data was developed using ab133673, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Alpha SNAP antibody [EPR7372] (<a href='/en-us/products/primary-antibodies/alpha-snap-antibody-epr7372-ab133673'>ab133673</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Molt4 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 33 kDa

false

OI-RD Scanning - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (AB248607)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (AB248607)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7372

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Perform antigen retrieval before commencing with IHC staining protocol.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab248607 is the carrier-free version of ab133673.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha SNAP also known as NSF attachment protein alpha is an important component of cellular machinery. This protein weighs approximately 33 kDa and facilitates membrane trafficking processes. It is expressed ubiquitously meaning it is found throughout various tissues in the body. Alpha SNAP is essential for the proper function of membrane fusion events playing a significant role in exocytosis and endocytosis.
Biological function summary

Alpha SNAP works as a part of the SNARE complex. It acts as a mediator that helps recycle SNARE proteins which are key components in vesicle fusion processes. The protein binds to the SNARE complex allowing NSF (N-ethylmaleimide-sensitive factor) to disassemble it enabling the components to be reused. This recycling is critical for continuous vesicular transport aiding in the maintenance of cellular function.

Pathways

Alpha SNAP functions mainly in the vesicular transport and membrane trafficking pathways. It interacts with NSF and other SNARE proteins to facilitate these processes. Within the cellular transport pathway Alpha SNAP ensures efficient neurotransmitter release and hormone secretion. It is involved in the endocytic and exocytic pathways both vital for proper cellular communication and function.

Mutations or dysfunctions in Alpha SNAP have been linked to neurological disorders such as epilepsy due to its role in neurotransmitter release. Its malfunction can disrupt synaptic communication leading to abnormal neural activity. Additionally Alpha SNAP may be implicated in some types of neurodegenerative diseases interacting with proteins such as NSF which are important for synaptic maintenance and plasticity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus (Probable). Together with GNA12 promotes CDH5 localization to plasma membrane (PubMed : 15980433).
See full target information NAPA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 10:e727 PubMed36301031

2022

Forkhead box M1 recruits FoxP3 Treg cells to induce immune escape in hilar cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ma,Zhaowei Sun,Xueliang Li,Jingyun Guo,Qinlei Wang,Mujian Teng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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