Rabbit Recombinant Monoclonal Alpha SNAP antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Images
![Western blot - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (AB248607), expandable thumbnail](https://content.abcam.com/products/images/alpha-snap-antibody-epr7372-bsa-and-azide-free-ab248607--western-blot-img105311.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (AB248607)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (AB248607), expandable thumbnail](https://content.abcam.com/products/images/alpha-snap-antibody-epr7372-bsa-and-azide-free-ab248607--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124861.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (AB248607)")
![Immunocytochemistry/ Immunofluorescence - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (AB248607), expandable thumbnail](https://content.abcam.com/products/images/alpha-snap-antibody-epr7372-bsa-and-azide-free-ab248607--immunocytochemistry-immunofluorescence-img3705.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (AB248607)")
![OI-RD Scanning - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (AB248607), expandable thumbnail](https://content.abcam.com/products/images/alpha-snap-antibody-epr7372-bsa-and-azide-free-ab248607--oi-rd-scanning-img97719.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (AB248607)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Tested |
| Mouse | Predicted | Predicted | Not recommended | Not recommended | Predicted |
| Rat | Predicted | Predicted | Not recommended | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform antigen retrieval before commencing with IHC staining protocol. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus (Probable). Together with GNA12 promotes CDH5 localization to plasma membrane (PubMed:15980433).
Alternative names
SNAPA, NAPA, Alpha-soluble NSF attachment protein, SNAP-alpha, N-ethylmaleimide-sensitive factor attachment protein alpha
Recommended products
Rabbit Recombinant Monoclonal Alpha SNAP antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR7372
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 9.3 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248607 is the carrier-free version of Anti-Alpha SNAP antibody [EPR7372] ab133673.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha SNAP also known as NSF attachment protein alpha is an important component of cellular machinery. This protein weighs approximately 33 kDa and facilitates membrane trafficking processes. It is expressed ubiquitously meaning it is found throughout various tissues in the body. Alpha SNAP is essential for the proper function of membrane fusion events playing a significant role in exocytosis and endocytosis.
- Biological function summary
Alpha SNAP works as a part of the SNARE complex. It acts as a mediator that helps recycle SNARE proteins which are key components in vesicle fusion processes. The protein binds to the SNARE complex allowing NSF (N-ethylmaleimide-sensitive factor) to disassemble it enabling the components to be reused. This recycling is critical for continuous vesicular transport aiding in the maintenance of cellular function.
- Pathways
Alpha SNAP functions mainly in the vesicular transport and membrane trafficking pathways. It interacts with NSF and other SNARE proteins to facilitate these processes. Within the cellular transport pathway Alpha SNAP ensures efficient neurotransmitter release and hormone secretion. It is involved in the endocytic and exocytic pathways both vital for proper cellular communication and function.
- Associated diseases and disorders
Mutations or dysfunctions in Alpha SNAP have been linked to neurological disorders such as epilepsy due to its role in neurotransmitter release. Its malfunction can disrupt synaptic communication leading to abnormal neural activity. Additionally Alpha SNAP may be implicated in some types of neurodegenerative diseases interacting with proteins such as NSF which are important for synaptic maintenance and plasticity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (ab248607), expandable thumbnail](https://content.abcam.com/products/images/alpha-snap-antibody-epr7372-bsa-and-azide-free-ab248607--western-blot-img105311.jpg "Western blot - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (ab248607)")
Western blot - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (ab248607)
This data was developed using Anti-Alpha SNAP antibody [EPR7372] ab133673, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Alpha SNAP antibody [EPR7372] (Anti-Alpha SNAP antibody [EPR7372] ab133673) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Molt4 cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 33 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (ab248607), expandable thumbnail](https://content.abcam.com/products/images/alpha-snap-antibody-epr7372-bsa-and-azide-free-ab248607--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124861.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (ab248607)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (ab248607)
This data was developed using Anti-Alpha SNAP antibody [EPR7372] ab133673, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Alpha SNAP with Anti-Alpha SNAP antibody [EPR7372] ab133673 at 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (ab248607), expandable thumbnail](https://content.abcam.com/products/images/alpha-snap-antibody-epr7372-bsa-and-azide-free-ab248607--immunocytochemistry-immunofluorescence-img3705.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (ab248607)")
Immunocytochemistry/ Immunofluorescence - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (ab248607)
This data was developed using Anti-Alpha SNAP antibody [EPR7372] ab133673, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HepG2 cells labelling Alpha SNAP with Anti-Alpha SNAP antibody [EPR7372] ab133673 at 1/100.
![OI-RD Scanning - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (ab248607), expandable thumbnail](https://content.abcam.com/products/images/alpha-snap-antibody-epr7372-bsa-and-azide-free-ab248607--oi-rd-scanning-img97719.jpg "OI-RD Scanning - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (ab248607)")
OI-RD Scanning - Anti-Alpha SNAP antibody [EPR7372] - BSA and Azide free (ab248607)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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