Anti-alpha-synuclein aggregate antibody [MJFR-14-6-4-2] is a rabbit monoclonal antibody that is used in SNCA Immunohistochemistry (IHC) and immunocytochemistry/immunofluorescence (ICC/IF). Suitable for human, mouse, rat, and more samples.
- Cited in over 70 publications
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone MJFR-14-6-4-2 is cited in over 90 publications
Alpha-synuclein aggregate MJFR-14-6-4-2 was developed with support from the Michael J. Fox Foundation for Parkinson’s Research.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (AB209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--immunocytochemistry-immunofluorescence-img66231.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (AB209538)")
![Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (AB209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--immunocytochemistry-immunofluorescence-img71960.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (AB209538)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (AB209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119404.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (AB209538)")
![Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (AB209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--immunocytochemistry-immunofluorescence-img88890.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (AB209538)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (AB209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24195.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (AB209538)")
Publications
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- Scientific reports 13:226132023Impaired neuronal activity as a potential factor contributing to the underdeveloped cerebrovasculature in a young Parkinson's disease mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Young Jeong,Hyun Jung Lee,Namsuk Kim,Yan Li,Jong-Cheol Rah,Won-Jong OhPubMed 38114623
- NPJ Parkinson's disease 9:1642023Sex-dimorphic neuroprotective effect of CD163 in an α-synuclein mouse model of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara A Ferreira,Conghui Li,Ida H Klæstrup,Zagorka Vitic,Rikke K Rasmussen,Asger Kirkegaard,Gitte U Toft,Cristine Betzer,Pia Svendsen,Poul H Jensen,Yonglun Luo,Anders Etzerodt,Søren K Moestrup,Marina Romero-RamosPubMed 38092806
- NPJ Parkinson's disease 9:1482023Characterization of the pathogenic α-Synuclein Variant V15A in Parkinson´s disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSokhna Haissatou Diaw,Max Borsche,Linn Streubel-Gallasch,Marija Dulovic-Mahlow,Julia Hermes,Insa Lenz,Philip Seibler,Christine Klein,Norbert Brüggemann,Melissa Vos,Katja LohmannPubMed 37903765
- Frontiers in neurology 14:11730742023Erythrocytic alpha-synuclein as potential biomarker for the differentiation between essential tremor and Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenwei Yu,Genliang Liu,Yuanchu Zheng,Guoshi Huang,Tao FengPubMed 37693770
- Frontiers in molecular biosciences 10:11775562023Sarkosyl differentially solubilizes patient-derived alpha-synuclein fibril strains.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHjalte Gram,Vasileios Theologidis,Thomas Boesen,Poul Henning JensenPubMed 37621995
- Molecular neurodegeneration 18:412023Targeted degradation of ⍺-synuclein aggregates in Parkinson's disease using the AUTOTAC technology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJihoon Lee,Ki Woon Sung,Eun-Jin Bae,Dabin Yoon,Dasarang Kim,Jin Saem Lee,Da-Ha Park,Daniel Youngjae Park,Su Ran Mun,Soon Chul Kwon,Hye Yeon Kim,Joo-Ok Min,Seung-Jae Lee,Young Ho Suh,Yong Tae KwonPubMed 37355598
- Biomolecules & therapeutics 31:417-42420236-Shogaol, an Active Ingredient of Ginger, Improves Intestinal and Brain Abnormalities in -Induced Parkinson's Disease Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEugene Huh,Jin Gyu Choi,Yujin Choi,In Gyoung Ju,Dongjin Noh,Dong-Yun Shin,Dong Hyun Kim,Hi-Joon Park,Myung Sook OhPubMed 37337830
- Journal of neuroinflammation 20:932023The cervical lymph node contributes to peripheral inflammation related to Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZongran Liu,Yang Huang,Xuejing Wang,Jia-Yi Li,Can Zhang,Ying Yang,Jing ZhangPubMed 37038192
- NPJ Parkinson's disease 9:422023DOPAL initiates αSynuclein-dependent impaired proteostasis and degeneration of neuronal projections in Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Masato,Nicoletta Plotegher,Francesca Terrin,Michele Sandre,Gaia Faustini,Andrea Thor,Stephen Adams,Giulia Berti,Susanna Cogo,Federica De Lazzari,Camilla Maria Fontana,Paul Anthony Martinez,Randy Strong,Rina Bandopadhyay,Marco Bisaglia,Arianna Bellucci,Elisa Greggio,Luisa Dalla Valle,Daniela Boassa,Luigi BubaccoPubMed 36966140
- Glycobiology 33:325-3412023Interplay between APP and glypican-1 processing and α-synuclein aggregation in undifferentiated and differentiated human neural progenitor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang Cheng,Lars-Åke Fransson,Katrin ManiPubMed 36790131
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Not recommended | Tested | Tested |
| Mouse | Expected | Not recommended | Tested | Expected |
| Rat | Expected | Not recommended | Tested | Expected |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 0.0022 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:20798282, PubMed:26442590, PubMed:28288128, PubMed:30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).
Alternative names
NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP
Recommended products
Anti-alpha-synuclein aggregate antibody [MJFR-14-6-4-2] is a rabbit monoclonal antibody that is used in SNCA Immunohistochemistry (IHC) and immunocytochemistry/immunofluorescence (ICC/IF). Suitable for human, mouse, rat, and more samples.
- Cited in over 70 publications
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone MJFR-14-6-4-2 is cited in over 90 publications
Alpha-synuclein aggregate MJFR-14-6-4-2 was developed with support from the Michael J. Fox Foundation for Parkinson’s Research.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
MJFR-14-6-4-2
- Purification technique
Affinity purification Protein A
- Specificity
This antibody mainly recognizes alpha synuclein Filament, but also has weak cross-reactivity with alpha synuclein Monomer.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Dot blot, ICC/IF, IHC-P in human, mouse, rat samples.
Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538) has been cited over 73 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Alpha-synuclein aggregate antibody (Anti-SNCA antibody) [MJFR-14-6-4-2] - Conformation-Specific (ab209538) has 6 independent reviews from customers.
Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538) specifically detects Alpha-synuclein aggregate (UniProt ID: P37840; Molecular weight: 14kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone MJFR-14-6-4-2 - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - BSA and Azide free ab214033.
Antibody clone MJFR-14-6-4-2 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Biotin, Alexa Fluor® 594 (Alexa Fluor® 488 Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific ab216124, Alexa Fluor® 647 Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific ab216309, Biotin Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific ab227047, Alexa Fluor® 594 Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] ab283628).
Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538) was developed by Abcam in partnership with the Michael J. Fox Foundation for Parkinson's Research.
ab209538 is not suitable for WB or other denaturing conditions, as it is conformation-specific.
This antibody is useful for studying Parkinson's disease and other synucleinopathies including dementia with Lewy bodies and multiple system atrophy.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody was developed with support from The Michael J. Fox Foundation.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-synuclein aggregates are critical in the study of neurobiology. These aggregates form by accumulation of alpha-synuclein a protein that exists mostly in neural tissues. Also known as SNCA this protein has a mass of approximately 14 kDa. Researchers frequently find alpha-synuclein in presynaptic terminals of neurons but expression also occurs in the nucleus and cytoplasm of neuronal cells. This protein has a notable interaction with lipid membranes indicating its role in synaptic vesicle regulation.
- Biological function summary
Alpha-synuclein manages several cellular processes most notably in neurotransmitter release and vesicle recycling. It does not work alone; it often forms complexes with other proteins like synaptobrevin-2 of the SNARE complex. The formation of these complexes allows for the proper functioning of synaptic vesicles. Alteration in alpha-synuclein's normal function through aggregation disrupts these processes which underlies many neural complications.
- Pathways
Many neuronal functions depend on alpha-synuclein's participation in synaptic vesicle pathways. Key pathways include the dopamine metabolic pathway where alpha-synuclein regulates dopamine release. The protein interacts with other synaptic proteins like the SNARE proteins potentially affecting protein kinase pathways. Abnormal aggregation impacts these pathways which can propagate cellular stress and contribute to cellular degeneration.
- Associated diseases and disorders
Alpha-synuclein aggregation correlates strongly with Parkinson's disease and Lewy body dementia. In these conditions aggregates form Lewy bodies protein clusters within neurons that impair their function and encourage neural degradation. The protein interacts with structural proteins like ubiquitin. Ubiquitin involvement highlights the importance of degradation pathways in these disorders as impaired proteostasis due to aggregated alpha-synuclein exacerbates disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--immunocytochemistry-immunofluorescence-img66231.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)")
Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)
Clone MJFR-14-6-4-2 (Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - BSA and Azide free ab214033) has been successfully conjugated by Abcam. This image was generated using Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (Alexa Fluor® 488). Please refer to Alexa Fluor® 488 Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific ab216124 for protocol details.
IHC image of alpha-synuclein aggregate staining in a section of frozen Parkinson human substantia nigra*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with Alexa Fluor® 488 Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific ab216124 at 1/100 dilution (shown in green) and counterstained using Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
![Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--immunocytochemistry-immunofluorescence-img71960.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)")
Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)
Clone MJFR-14-6-4-2 (Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - BSA and Azide free ab214033) has been successfully conjugated by Abcam. This image was generated using Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific ab216309 for protocol details.
IHC image of alpha-synuclein aggregate staining in a section of formalin-fixed paraffin-embedded Parkinson human substantia nigra*.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110oC for 8 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with Alexa Fluor® 647 Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific ab216309 at 1/5000 dilution (shown in red) and counterstained using Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119404.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)")
This image is courtesy of a customer review by Carl Hobbs.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)
ab209538 staining Alpha-synuclein aggregate in rat dorsal root ganglian (DRG) tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, cut into 20 micron slices and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/2000 in TBS/BSA/azide) at 21°C for 2 hours. An Alexa Fluor® 555-conjugated Goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--immunocytochemistry-immunofluorescence-img88890.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)")
Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized ReNcell VM (Human neural progenitor) cells labeling Alpha-synuclein aggregate with ab209538 at 1/5000 dilution, followed by Alexa Fluor® 647 Donkey anti-Rabbit IgG (H+L) secondary antibody at 1/400 dilution (red).
Upper panel counterstain: Anti-aggregated alpha-synuclein antibody clone 5G4 at 1/400 dilution, followed by AlexaFluor®488 secondary detection (green).
Lower panel counterstain: Anti-alpha/beta-synculein antibody at 1/200 dilution, followed by Alexa Fluor® 488 secondary detection (green).
Blocking buffer: 3% bovine serum albumin and 2% fetal bovine serum.
ReNcell VM cells were differentiated in media containing cAMP and GDNF (without bFGF or EGF) and transduced with Ad5C01 viral vector encoding human WT alpha-synuclein filament.
Images were reproduced courtesy of Charles River.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24195.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)")
This image is courtesy of a customer review submitted by Carl Hobbs.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)
ab209538 staining Alpha-synuclein aggregate in mouse colon tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, cut into 20 micron slices, blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/2000 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--immunocytochemistry-immunofluorescence-img41868.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)")
Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized ReNcell VM (Human neural progenitor) cells labeling Alpha-synuclein aggregate with ab209538 at 1/5000 dilution, followed by Alexa Fluor® 647 Donkey anti-Rabbit IgG (H+L) secondary antibody at 1/400 dilution (red).
Blocking buffer: 3% bovine serum albumin and 2% fetal bovine serum.
ReNcell VM cells were differentiated in media containing cAMP and GDNF (without bFGF or EGF) and transduced with Ad5C01 viral vector encoding human alpha-synuclein filament (Left image). Right image show control vector cells.
Images were reproduced courtesy of Charles River.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91301.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)")
This image is courtesy of a customer review submitted by Carl HobbsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)
ab209538 staining Alpha-synuclein aggregate in Human DLB brain tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was fixed with paraformaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in Citric acid. Samples were incubated with primary antibody (1/10,000 in TBS) for 2 hours at 21°C. A biotin conjugated anti-rabbit IgG Goat polyclonal was used as the secondary antibody at 1/300 dilution.
![Dot Blot - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--dot-blot-img29939.jpg "Dot Blot - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)")
Dot Blot - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)
Dot blot analysis of alpha-synuclein filament labeled with ab209538 at 2.2 ng/ml.
Lane 1: Recombinant alpha-synuclein filament treated with 70% formic acid.
Lane 2: Recombinant alpha-synuclein filament treated with 70% formic acid.
Lane 3: Untreated recombinant alpha-synuclein filament.
Lane 4: Untreated recombinant alpha-synuclein filament.
Alpha Synuclein filaments were generated using full length recombinant alpha-synuclein (aa1-140) by incubation in 20mM TRIS, pH 7.2 at 37 °C with agitation.
Denaturation of filaments with 70% formic acid reduces antibody recognition by 30-100 fold, demonstrating conformation specificity.
Data is provided by Professor Poul Henning Jensen, Aarhus University, Denmark.
![Dot Blot - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--dot-blot-img42275.jpg "Dot Blot - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)")
Data is provided by Professor Poul Henning Jensen, Aarhus University, Denmark.Dot Blot - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)
Dot Blot showing the reactivity of ab209538 (2 ng/ml) with
F: alpha synuclein Filament
F+FA: alpha synuclein Filament treated in 50% formic acid for 1 h 37oC prior to application to the dot blot.
M+FA: alpha synuclein Monomer treated in 50% formic acid for 1 h 37oC prior to application to the dot blot.
Loading control antibody (1:1000) reacts with Alpha-synuclein irrespectively of it being in a filament, oligomer or a monomer.
![Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--immunocytochemistry-immunofluorescence-img41868.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91301.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)")
![Dot Blot - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--dot-blot-img29939.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)")
![Dot Blot - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-aggregate-antibody-mjfr-14-6-4-2-conformation-specific-ab209538--dot-blot-img42275.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (ab209538)")
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