Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (AB280382)

This data was developed using ab280377 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Alpha-synuclein with ab280377 at 1/5000 (0.221 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining in human cerebrum.The section was incubated with ab280377 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (AB280382)

This data was developed using ab280377 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Alpha-synuclein with ab280377 at 1/5000 (0.221 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining in mouse cerebrum.The section was incubated with ab280377 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (AB280382)

This data was developed using ab280377 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Alpha-synuclein with ab280377 at 1/5000 (0.221 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining in rat cerebrum.The section was incubated with ab280377 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• WB

Supplier Data

Western blot - Anti-Alpha-synuclein antibody [42/a-Synuclein] - BSA and Azide free (AB280382)

This data was developed using ab280377, the same antibody clone in a different buffer formulation.

Blocking and Diluting buffer and concentration : 5% NFDM/TBST

Negative control : mouse kidney, human kidney, rat kidney (PMID : 14997013)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 14997013, 30553024).

Exposure time 15 seconds

All lanes:

Western blot - Anti-Alpha-synuclein antibody [42/a-Synuclein] (<a href='/en-us/products/primary-antibodies/alpha-synuclein-antibody-42-a-synuclein-ab280377'>ab280377</a>) at 1/1000 dilution

Lane 1:

Mouse cerebellum lysate at 10 µg

Lane 2:

Mouse kidney lysate at 10 µg

Lane 3:

Human cerebellum lysate at 10 µg

Lane 4:

Human kidney lysate at 10 µg

Lane 5:

Rat hippocampus lysate at 10 µg

Lane 6:

Rat kidney lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 18 kDa

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