Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903) is a mouse monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Alpha-synuclein in Western Blot, IHC-P. Suitable for Human.
- BSA, sodium azide, and glycerol-free for easy conjugation
- Over 70 publications
- Trusted since 2004
Images
![Western blot - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (AB1903), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-4d6-bsa-and-azide-free-ab1903--western-blot-img7159.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (AB1903)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (AB1903), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-4d6-bsa-and-azide-free-ab1903--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img43513.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (AB1903)")
Publications
Filter by
- Science advances 9:eadi87162023Anionic nanoplastic contaminants promote Parkinson's disease-associated α-synuclein aggregation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiyong Liu,Arpine Sokratian,Addison M Duda,Enquan Xu,Christina Stanhope,Amber Fu,Samuel Strader,Huizhong Li,Yuan Yuan,Benjamin G Bobay,Joana Sipe,Ketty Bai,Iben Lundgaard,Na Liu,Belinda Hernandez,Catherine Bowes Rickman,Sara E Miller,Andrew B WestPubMed 37976362
- iScience 26:1081302023LRRK2 G2019S promotes astrocytic inflammation induced by oligomeric α-synuclein through NF-κB pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai-Jie He,Jin-Bao Zhang,Jun-Yi Liu,Feng-Lun Zhao,Xiao-Yu Yao,Yu-Ting Tang,Jin-Ru Zhang,Xiao-Yu Cheng,Li-Fang Hu,Fen Wang,Chun-Feng LiuPubMed 37876795
- Pharmaceutics 15:2023Targeted Extracellular Vesicle Gene Therapy for Modulating Alpha-Synuclein Expression in Gut and Spinal Cord.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Izco,Martin Schleef,Marco Schmeer,Estefania Carlos,Guglielmo Verona,Lydia Alvarez-ErvitiPubMed 37111717
- International journal of molecular sciences 24:2023DDC-Promoter-Driven Chemogenetic Activation of SNpc Dopaminergic Neurons Alleviates Parkinsonian Motor Symptoms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong-Chan Seo,Yeon Ha Ju,Jin-Ju Seo,Soo-Jin Oh,C Justin Lee,Seung Eun Lee,Min-Ho NamPubMed 36768816
- Movement disorders : official journal of the Movement Disorder Society 38:444-4522023Pathological Validation of the MDS Criteria for the Diagnosis of Multiple System Atrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSasivimol Virameteekul,Tamas Revesz,Zane Jaunmuktane,Thomas T Warner,Eduardo De Pablo-FernándezPubMed 36606594
- Movement disorders : official journal of the Movement Disorder Society 38:558-5662023Clinical Diagnostic Accuracy of Parkinson's Disease: Where Do We Stand?Applications:Unspecified applicationReactive species:Unspecified reactive speciesSasivimol Virameteekul,Tamas Revesz,Zane Jaunmuktane,Thomas T Warner,Eduardo De Pablo-FernándezPubMed 36602274
- PloS one 17:e02776582022Human pericytes degrade diverse α-synuclein aggregates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBirger Victor Dieriks,Blake Highet,Ania Alik,Tracy Bellande,Taylor J Stevenson,Victoria Low,Thomas I-H Park,Jason Correia,Patrick Schweder,Richard L M Faull,Ronald Melki,Maurice A Curtis,Mike DragunowPubMed 36399706
- Acta neuropathologica communications 10:1342022Mitochondrial dysfunction is a key pathological driver of early stage Parkinson's.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristina E Toomey,Wendy E Heywood,James R Evans,Joanne Lachica,Sarah N Pressey,Sandrine C Foti,Mesfer Al Shahrani,Karishma D'Sa,Iain P Hargreaves,Simon Heales,Michael Orford,Claire Troakes,Johannes Attems,Ellen Gelpi,Miklos Palkovits,Tammaryn Lashley,Steve M Gentleman,Tamas Revesz,Kevin Mills,Sonia GandhiPubMed 36076304
- Molecular therapy. Nucleic acids 29:1-152022Suppression of abnormal α-synuclein expression by activation of BDNF transcription ameliorates Parkinson's disease-like pathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianqian Cao,Shilin Luo,Wei Yao,Youge Qu,Nanbu Wang,Jian Hong,Shigeo Murayama,Zhentao Zhang,Jiaxu Chen,Kenji Hashimoto,Qi Qi,Ji-Chun ZhangPubMed 35784012
- Cell reports 39:1106752022Synaptic vesicle binding of α-synuclein is modulated by β- and γ-synucleins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKathryn E Carnazza,Lauren E Komer,Ying Xue Xie,André Pineda,Juan Antonio Briano,Virginia Gao,Yoonmi Na,Trudy Ramlall,Vladimir L Buchman,David Eliezer,Manu Sharma,Jacqueline BurréPubMed 35417693
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human SNCA. Database link P37840
Reactivity data
Select an application| IHC-P | WB | ICC | Flow Cyt | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Antigen retrieval is not essential but may optimise staining. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/10000 | Notes - |
ICC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor
Recommended products
Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903) is a mouse monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Alpha-synuclein in Western Blot, IHC-P. Suitable for Human.
- BSA, sodium azide, and glycerol-free for easy conjugation
- Over 70 publications
- Trusted since 2004
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human SNCA. Database link P37840
- Carrier free
- Yes
- Clone number
- 4D6
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Ammonium sulfate precipitated and dialyzed tissue culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of Alpha-synuclein?
Anti-Alpha-synuclein [4D6] - BSA and Azide free (ab1903) specifically detects a band for Alpha-synuclein (UniProt: P37840) at a molecular weight of 16kDa.
Trusted by the scientific community
Anti-Alpha-synuclein [4D6] - BSA and Azide free (ab1903) was first used in a scientific publication in 2004 and has been cited over 70 times in peer-reviewed journals.
Reviewed by scientists
Anti-Alpha-synuclein [4D6] - BSA and Azide free (ab1903) has over 5 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
- Biological function summary
The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.
- Pathways
Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.
- Associated diseases and disorders
Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-4d6-bsa-and-azide-free-ab1903--western-blot-img7159.jpg "Western blot - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903)")
Western blot - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903)
All lanes: Western blot - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903) at 5 µg/mL
Lane 1: A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg
Lane 2: SK N BE (Human neuroblastoma) Whole Cell Lysate at 10 µg
Lane 3: SK N SH (Human neuroblastoma) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 14 kDa
Observed band size: 16 kDa, 18 kDa, 80 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-4d6-bsa-and-azide-free-ab1903--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img43513.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903)
IHC-P using ab1903 showing lewy bodies in a human substantia nigra neuron(x400)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com