AB131508

Anti-Alpha-synuclein antibody

Name: Anti-Alpha-synuclein antibody (ab131508) | Abcam
Brand: Abcam Limited
SKU: AB131508
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Polyclonal Alpha-synuclein antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications.

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein antibody (AB131508)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein antibody (AB131508)

Immunofluorescence analysis of methanol fixed HeLa cells labelled with ab131508 at 1/100 dilution.

Unknown

Western blot - Anti-Alpha-synuclein antibody (AB131508)

All lanes:

Western blot - Anti-Alpha-synuclein antibody (ab131508) at 1/500 dilution

Lane 1:

HeLa cell extract

Lane 2:

3T3 cell extract

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects endogenous levels of total alpha Synuclein. Due to 72.7% sequence homology ab131508 might react with Beta synuclein

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.

See full target information SNCA

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 18:1366098 PubMed38644975

2024

Micro-and mesoscale aspects of neurodegeneration in engineered human neural networks carrying the LRRK2 G2019S mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Vibeke Devold Valderhaug,Ola Huse Ramstad,Rosanne van de Wijdeven,Kristine Heiney,Stefano Nichele,Axel Sandvig,Ioanna Sandvig

Biomolecules 12: PubMed35204664

2022

Spreading of Aggregated α-Synuclein in Sagittal Organotypic Mouse Brain Slices.

Applications

Unspecified application

Species

Unspecified reactive species

Buket Uçar,Nadia Stefanova,Christian Humpel

Scientific reports 11:15934 PubMed34354200

2021

Novel blood test for early biomarkers of preeclampsia and Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Shibin Cheng,Sayani Banerjee,Lori A Daiello,Akitoshi Nakashima,Sukanta Jash,Zheping Huang,Jonathan D Drake,Jan Ernerudh,Goran Berg,James Padbury,Shigeru Saito,Brian R Ott,Surendra Sharma

American journal of physiology. Cell physiology 320:C1141-C1152 PubMed33950697

2021

Early functional changes associated with alpha-synuclein proteinopathy in engineered human neural networks.

Applications

Unspecified application

Species

Unspecified reactive species

Vibeke D Valderhaug,Kristine Heiney,Ola Huse Ramstad,Geir Bråthen,Wei-Li Kuan,Stefano Nichele,Axel Sandvig,Ioanna Sandvig

Journal of controlled release : official journal o 315:139-149 PubMed31678095

2019

GDNF-expressing macrophages restore motor functions at a severe late-stage, and produce long-term neuroprotective effects at an early-stage of Parkinson's disease in transgenic Parkin Q311X(A) mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuling Zhao,Matthew J Haney,Yeon S Jin,Olga Uvarov,Natasha Vinod,Yueh Z Lee,Benjamin Langworthy,Jason P Fine,Myosotys Rodriguez,Nazira El-Hage,Alexander V Kabanov,Elena V Batrakova

Scientific reports 7:13631 PubMed29051583

2017

Nuclear fragments of the neural cell adhesion molecule NCAM with or without polysialic acid differentially regulate gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Westphal,Thomas Theis,Gabriele Loers,Melitta Schachner,Ralf Kleene

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alpha-synuclein antibody

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein antibody (AB131508)

Western blot - Anti-Alpha-synuclein antibody (AB131508)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise