AB52168

Anti-Alpha-synuclein antibody

Name: Anti-Alpha-synuclein antibody (ab52168) | Abcam
Brand: Abcam Limited
SKU: AB52168
Price: 565 USD
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(15 Publications)

Rabbit Polyclonal Alpha-synuclein antibody. Suitable for IHC-P and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human SNCA.

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (AB52168)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (AB52168)

ab52168 staining human dentate nuleus. Staining is localised to the cytoplasm and nucleus.
Left panel : with primary antibody at 4 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be r

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human SNCA. The exact immunogen used to generate this antibody is proprietary information.

P37840

Specificity

Due to 83% sequence homology ab52168 might react with Beta synuclein

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed : 20798282, PubMed : 26442590, PubMed : 28288128, PubMed : 30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed : 28288128, PubMed : 30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed : 30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed : 20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed : 26442590).

See full target information SNCA

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7797 PubMed40841722

2025

Integrating artificial intelligence and optogenetics for Parkinson's disease diagnosis and therapeutics in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bobae Hyeon,Jaehyun Shin,Jae-Hun Lee,Woori Kim,Jea Kwon,Heeyoung Lee,Dae-Gun Kim,Choong Yeon Kim,Sian Choi,Jae-Woong Jeong,Kwang-Soo Kim,C Justin Lee,Daesoo Kim,Won Do Heo

Scientific reports 15:9992 PubMed40121347

2025

Viral mediated α-synuclein overexpression results in greater transgene levels and α-synuclein overload in mice bearing kinase dead mutation of LRRK2.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Albanese,Chiara Domenicale,Daniela Mercatelli,Alberto Brugnoli,Sandra Dovero,Erwan Bezard,Michele Morari

Iranian journal of pharmaceutical research : IJPR 23:e144200 PubMed39830652

2025

Insulin and TLR4 Inhibitor Improve Motor Impairments in a Rat Model of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemeh Hemmati,Neda Valian,Abolhassan Ahmadiani,Zahurin Mohamed,Raymond Azman Ali,Norlinah Mohamed Ibrahim,Seyed Farshad Hosseini Shirazi

Biomedicines 10: PubMed35453631

2022

Dopamine Transporter, PhosphoSerine129 α-Synuclein and α-Synuclein Levels in Aged LRRK2 G2019S Knock-In and Knock-Out Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Domenicale,Daniela Mercatelli,Federica Albanese,Salvatore Novello,Fabrizio Vincenzi,Katia Varani,Michele Morari

PeerJ 10:e13256 PubMed35433120

2022

Icaritin attenuates 6-OHDA-induced MN9D cell damage by inhibiting oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Zhou,Nanqu Huang,Xiaoyi Hou,Li Zhu,Yiman Xie,Zhisheng Ba,Yong Luo

Nature communications 12:1814 PubMed33753734

2021

The release of toxic oligomers from α-synuclein fibrils induces dysfunction in neuronal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Cascella,Serene W Chen,Alessandra Bigi,José D Camino,Catherine K Xu,Christopher M Dobson,Fabrizio Chiti,Nunilo Cremades,Cristina Cecchi

Parkinsonism & related disorders 84:61-67 PubMed33571872

2021

Compound heterozygous variants in Wiskott-Aldrich syndrome like (WASL) gene segregating in a family with early onset Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sumeet Kumar,Masoom M Abbas,Shyla T Govindappa,Uday B Muthane,Madhuri Behari,Sanjay Pandey,Ramesh C Juyal,B K Thelma

Bone 137:115428 PubMed32417536

2020

Deletion of α-Synuclein in Prrx1-positive cells causes partial loss of function in the central nervous system (CNS) but does not affect ovariectomy induced bone loss.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina A Figueroa,Pratima Bajgain,J Patrizia Stohn,Arturo Hernandez,Daniel J Brooks,Karen L Houseknecht,Clifford J Rosen

eLife 8: PubMed31310234

2019

Electroporated recombinant proteins as tools for in vivo functional complementation, imaging and chemical biology.

Applications

Unspecified application

Species

Unspecified reactive species

Amal Alex,Valentina Piano,Soumitra Polley,Marchel Stuiver,Stephanie Voss,Giuseppe Ciossani,Katharina Overlack,Beate Voss,Sabine Wohlgemuth,Arsen Petrovic,Yaowen Wu,Philipp Selenko,Andrea Musacchio,Stefano Maffini

The Journal of biological chemistry 294:9973-9984 PubMed31092553

2019

C-terminal α-synuclein truncations are linked to cysteine cathepsin activity in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan P McGlinchey,Shannon M Lacy,Katherine E Huffer,Nahid Tayebi,Ellen Sidransky,Jennifer C Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Alpha-synuclein antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (AB52168)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise