Rabbit Polyclonal Alpha-synuclein antibody. Suitable for IHC-P and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human SNCA.
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- PeerJ 10:e132562022Icaritin attenuates 6-OHDA-induced MN9D cell damage by inhibiting oxidative stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinyu Zhou,Nanqu Huang,Xiaoyi Hou,Li Zhu,Yiman Xie,Zhisheng Ba,Yong LuoPubMed 35433120
- Nature communications 12:18142021The release of toxic oligomers from α-synuclein fibrils induces dysfunction in neuronal cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoberta Cascella,Serene W Chen,Alessandra Bigi,José D Camino,Catherine K Xu,Christopher M Dobson,Fabrizio Chiti,Nunilo Cremades,Cristina CecchiPubMed 33753734
- Parkinsonism & related disorders 84:61-672021Compound heterozygous variants in Wiskott-Aldrich syndrome like (WASL) gene segregating in a family with early onset Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSumeet Kumar,Masoom M Abbas,Shyla T Govindappa,Uday B Muthane,Madhuri Behari,Sanjay Pandey,Ramesh C Juyal,B K ThelmaPubMed 33571872
- Bone 137:1154282020Deletion of α-Synuclein in Prrx1-positive cells causes partial loss of function in the central nervous system (CNS) but does not affect ovariectomy induced bone loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarolina A Figueroa et. al.PubMed 32417536
- eLife 8:2019Electroporated recombinant proteins as tools for in vivo functional complementation, imaging and chemical biology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmal Alex et. al.PubMed 31310234
- The Journal of biological chemistry 294:9973-99842019C-terminal α-synuclein truncations are linked to cysteine cathepsin activity in Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyan P McGlinchey et. al.PubMed 31092553
- Cell death & disease 10:1742019Microglia as modulators of exosomal alpha-synuclein transmission.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Xia et. al.PubMed 30787269
- Acta neuropathologica communications 5:222017Age-dependent dopamine transporter dysfunction and Serine129 phospho-α-synuclein overload in G2019S LRRK2 mice.
- Scientific reports 7:406992017DOPAL derived alpha-synuclein oligomers impair synaptic vesicles physiological function.
- Molecular biology of the cell 25:3870-832014The Golgi S-acylation machinery comprises zDHHC enzymes with major differences in substrate affinity and S-acylation activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKimon Lemonidis et. al.PubMed 25253725
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SNCA. The exact immunogen used to generate this antibody is proprietary information. Database link P37840
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Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
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Target data
Function
The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor
Recommended products
Rabbit Polyclonal Alpha-synuclein antibody. Suitable for IHC-P and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human SNCA.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SNCA. The exact immunogen used to generate this antibody is proprietary information. Database link P37840
- Purification technique
- Affinity purification Immunogen
- Specificity
Due to 83% sequence homology ab52168 might react with Beta synuclein
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
- Biological function summary
The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.
- Pathways
Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.
- Associated diseases and disorders
Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (ab52168)
ab52168 staining human dentate nuleus. Staining is localised to the cytoplasm and nucleus.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be r
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