AB6162

Anti-Alpha-synuclein antibody

Name: Anti-Alpha-synuclein antibody (ab6162) | Abcam
Brand: Abcam Limited
SKU: AB6162
Price: 600 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(38 Publications)

Sheep Polyclonal Alpha-synuclein antibody. Suitable for WB and reacts with Mouse, Recombinant full length protein - Human samples. Cited in 38 publications. Immunogen corresponding to Synthetic Peptide within Human SNCA aa 100-150.

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

1 Images

Supplier Data

Western blot - Anti-Alpha-synuclein antibody (AB6162)

All lanes:

Western blot - Anti-Alpha-synuclein antibody (ab6162) at 1 µg/mL

Lane 1:

Recombinant Human α -synuclein at 0.1 µg

Lane 2:

Mouse brain lysate at 30 µg

Secondary

All lanes:

anti-sheep HRP at 1/6000 dilution

Predicted band size: 14 kDa

false

Key facts

Host species

Sheep

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human SNCA aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P37840

Specificity

Human and rat alpha synuclein. Cross reactivity: This antibody is known to react with human, mouse, rat and other rodents. Cross reactivity with other species has not yet been tested.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 7.4

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.

See full target information SNCA

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Publications (38)

Recent publications for all applications. Explore the full list and refine your search

NPJ Parkinson's disease 10:1 PubMed38167744

2024

Aggregate-prone brain regions in Parkinson's disease are rich in unique N-terminus α-synuclein conformers with high proteolysis susceptibility.

Applications

Unspecified application

Species

Unspecified reactive species

James A Wiseman,Helen C Murray,Richard L M F Faull,Michael Dragunow,Clinton P Turner,Birger Victor Dieriks,Maurice A Curtis

JCI insight 8: PubMed38063197

2023

Gut mucosal cells transfer α-synuclein to the vagus nerve.

Applications

Unspecified application

Species

Unspecified reactive species

Rashmi Chandra,Arpine Sokratian,Katherine R Chavez,Stephanie King,Sandip M Swain,Joshua C Snyder,Andrew B West,Rodger A Liddle

Biomedicines 11: PubMed36831023

2023

A Proteome-Wide Effect of PHF8 Knockdown on Cortical Neurons Shows Downregulation of Parkinson's Disease-Associated Protein Alpha-Synuclein and Its Interactors.

Applications

Unspecified application

Species

Unspecified reactive species

Nicodemus E Oey,Lei Zhou,Christine Hui Shan Chan,Antonius M J VanDongen,Eng King Tan

Molecular neurobiology 59:1333-1344 PubMed34984583

2022

Cancerous Inhibitor of Protein Phosphatase 2A (CIP2A): Could It Be a Promising Biomarker and Therapeutic Target in Parkinson's Disease?

Applications

Unspecified application

Species

Unspecified reactive species

Sijia Yin,Chao Han,Yun Xia,Fang Wan,Junjie Hu,Liang Kou,Yadi Sun,Jiawei Wu,Yunna Li,Qiulu Zhou,Nian Xiong,Jinsha Huang,Tao Wang

mAbs 13:1994690 PubMed34709986

2021

Nonclinical safety evaluation, pharmacokinetics, and target engagement of Lu AF82422, a monoclonal IgG1 antibody against alpha-synuclein in development for treatment of synucleinopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Lone Fjord-Larsen,Annemette Thougaard,Karen Malene Wegener,Joan Christiansen,Frank Larsen,Lise Maj Schrøder-Hansen,Marianne Kaarde,Dorte Kornerup Ditlevsen

Journal of cellular and molecular medicine 25:9634-9646 PubMed34486218

2021

Prolyl oligopeptidase inhibition reduces alpha-synuclein aggregation in a cellular model of multiple system atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Hengjing Cui,Tommi Kilpeläinen,Lydia Zouzoula,Samuli Auno,Kalevi Trontti,Sampo Kurvonen,Susanna Norrbacka,Iiris Hovatta,Poul Henning Jensen,Timo T Myöhänen

The Journal of neuroscience : the official journal of the Society for Neuroscience 41:8725-8741 PubMed34462307

2021

The Role of Galanin in Cerebellar Granule Cell Migration in the Early Postnatal Mouse during Normal Development and after Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yutaro Komuro,Ludovic Galas,Yury M Morozov,Jennifer K Fahrion,Emilie Raoult,Alexis Lebon,Amanda K Tilot,Shin Kikuchi,Nobuhiko Ohno,David Vaudry,Pasko Rakic,Hitoshi Komuro

Movement disorders : official journal of the Movement Disorder Society 36:1911-1918 PubMed33876851

2021

Increased Accumulation of α-Synuclein in Inflamed Appendices of Parkinson's Disease Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhua Chen,Wei Wu,Shuli Zhao,Xinyi Lv,Ji Hu,Chao Han,Guoping Wang,Shicun Wang,Pan Bo,Juan Zhang,Wei Gui,Qiqiang Tang,Qiang Liu,Shu Zhu,Feng Yu

Journal of neuroinflammation 18:4 PubMed33402167

2021

Microglial activation contributes to cognitive impairments in rotenone-induced mouse Parkinson's disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Dongdong Zhang,Sheng Li,Liyan Hou,Lu Jing,Zhengzheng Ruan,Bingjie Peng,Xiaomeng Zhang,Jau-Shyong Hong,Jie Zhao,Qingshan Wang

Biomedicine & pharmacotherapy = Biomedecine & phar 131:110788 PubMed33152946

2020

Prolyl oligopeptidase inhibition by KYP-2407 increases alpha-synuclein fibril degradation in neuron-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jinar Rostami,Maria Jäntti,Hengjing Cui,Maiju K Rinne,Jyrki P Kukkonen,Anna Falk,Anna Erlandsson,Timo Myöhänen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Alpha-synuclein antibody

Western blot - Anti-Alpha-synuclein antibody (AB6162)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (38)

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