Sheep Polyclonal Alpha-synuclein antibody. Suitable for WB and reacts with Mouse, Recombinant full length protein - Human samples. Cited in 32 publications. Immunogen corresponding to Synthetic Peptide within Human SNCA aa 100-150.
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- JCI insight 8:2023Gut mucosal cells transfer α-synuclein to the vagus nerve.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRashmi Chandra,Arpine Sokratian,Katherine R Chavez,Stephanie King,Sandip M Swain,Joshua C Snyder,Andrew B West,Rodger A LiddlePubMed 38063197
- Biomedicines 11:2023A Proteome-Wide Effect of PHF8 Knockdown on Cortical Neurons Shows Downregulation of Parkinson's Disease-Associated Protein Alpha-Synuclein and Its Interactors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicodemus E Oey,Lei Zhou,Christine Hui Shan Chan,Antonius M J VanDongen,Eng King TanPubMed 36831023
- Molecular neurobiology 59:1333-13442022Cancerous Inhibitor of Protein Phosphatase 2A (CIP2A): Could It Be a Promising Biomarker and Therapeutic Target in Parkinson's Disease?Applications:Unspecified applicationReactive species:Unspecified reactive speciesSijia Yin,Chao Han,Yun Xia,Fang Wan,Junjie Hu,Liang Kou,Yadi Sun,Jiawei Wu,Yunna Li,Qiulu Zhou,Nian Xiong,Jinsha Huang,Tao WangPubMed 34984583
- mAbs 13:19946902021Nonclinical safety evaluation, pharmacokinetics, and target engagement of Lu AF82422, a monoclonal IgG1 antibody against alpha-synuclein in development for treatment of synucleinopathies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLone Fjord-Larsen,Annemette Thougaard,Karen Malene Wegener,Joan Christiansen,Frank Larsen,Lise Maj Schrøder-Hansen,Marianne Kaarde,Dorte Kornerup DitlevsenPubMed 34709986
- Journal of cellular and molecular medicine 25:9634-96462021Prolyl oligopeptidase inhibition reduces alpha-synuclein aggregation in a cellular model of multiple system atrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHengjing Cui,Tommi Kilpeläinen,Lydia Zouzoula,Samuli Auno,Kalevi Trontti,Sampo Kurvonen,Susanna Norrbacka,Iiris Hovatta,Poul Henning Jensen,Timo T MyöhänenPubMed 34486218
- The Journal of neuroscience : the official journal of the Society for Neuroscience 41:8725-87412021The Role of Galanin in Cerebellar Granule Cell Migration in the Early Postnatal Mouse during Normal Development and after Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYutaro Komuro,Ludovic Galas,Yury M Morozov,Jennifer K Fahrion,Emilie Raoult,Alexis Lebon,Amanda K Tilot,Shin Kikuchi,Nobuhiko Ohno,David Vaudry,Pasko Rakic,Hitoshi KomuroPubMed 34462307
- Movement disorders : official journal of the Movement Disorder Society 36:1911-19182021Increased Accumulation of α-Synuclein in Inflamed Appendices of Parkinson's Disease Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuhua Chen,Wei Wu,Shuli Zhao,Xinyi Lv,Ji Hu,Chao Han,Guoping Wang,Shicun Wang,Pan Bo,Juan Zhang,Wei Gui,Qiqiang Tang,Qiang Liu,Shu Zhu,Feng YuPubMed 33876851
- Journal of neuroinflammation 18:42021Microglial activation contributes to cognitive impairments in rotenone-induced mouse Parkinson's disease model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongdong Zhang,Sheng Li,Liyan Hou,Lu Jing,Zhengzheng Ruan,Bingjie Peng,Xiaomeng Zhang,Jau-Shyong Hong,Jie Zhao,Qingshan WangPubMed 33402167
- Neuroscience 419:129-1402019Lesion of the Locus Coeruleus Damages Learning and Memory Performance in Paraquat and Maneb-induced Mouse Parkinson's Disease Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiyan Hou et. al.PubMed 31634513
- Journal of molecular medicine (Berlin, Germany) 97:1329-13442019Exosomes from patients with Parkinson's disease are pathological in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Han et. al.PubMed 31302715
Key facts
- Isotype
- IgG
- Host species
- Sheep
- Storage buffer
pH: 7 - 7.4
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SNCA aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link P37840
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Reactivity data
Select an application| Flow Cyt | WB | ICC/IF | |
|---|---|---|---|
| Human | Not recommended | Predicted | Not recommended |
| Mouse | Not recommended | Tested | Not recommended |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.5 µg/mL | Notes - |
Species Recombinant full length protein - Human | Dilution info 0.5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
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7 products for Alternative Product
Target data
Function
The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor
Recommended products
Sheep Polyclonal Alpha-synuclein antibody. Suitable for WB and reacts with Mouse, Recombinant full length protein - Human samples. Cited in 32 publications. Immunogen corresponding to Synthetic Peptide within Human SNCA aa 100-150.
Key facts
- Isotype
- IgG
- Host species
- Sheep
- Storage buffer
pH: 7 - 7.4
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SNCA aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link P37840
- Purification technique
- Affinity purification Immunogen
- Specificity
Human and rat alpha synuclein. Cross reactivity: This antibody is known to react with human, mouse, rat and other rodents. Cross reactivity with other species has not yet been tested.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
- Biological function summary
The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.
- Pathways
Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.
- Associated diseases and disorders
Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Alpha-synuclein antibody (ab6162)
All lanes: Western blot - Anti-Alpha-synuclein antibody (ab6162) at 1 µg/mL
Lane 1: Recombinant Human α -synuclein at 0.1 µg
Lane 2: Mouse brain lysate at 30 µg
SecondaryAll lanes: anti-sheep HRP at 1/6000 dilution
Predicted band size: 14 kDa
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