Rabbit Recombinant Monoclonal Alpha-synuclein antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse, Human, Rat, Recombinant full length protein samples.
Images
![Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (AB225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--western-blot-img183710.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (AB225866)")
![Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (AB225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--western-blot-img175162.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (AB225866)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (AB225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111447.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (AB225866)")
![Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (AB225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--western-blot-img166793.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (AB225866)")
![Immunohistochemistry (Frozen sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (AB225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--immunohistochemistry-frozen-sections-img117859.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (AB225866)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Tested | Expected | Tested |
| Mouse | Tested | Tested | Tested | Tested |
| Rat | Expected | Tested | Expected | Tested |
| Recombinant full length protein | Expected | Expected | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Recombinant full length protein | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
Species Recombinant full length protein | Dilution info - | Notes Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).
Alternative names
Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP, PARK1, NACP, SNCA
Recommended products
Rabbit Recombinant Monoclonal Alpha-synuclein antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse, Human, Rat, Recombinant full length protein samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR20535
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab225866 is the carrier-free version of Anti-Alpha-synuclein antibody [EPR20535] ab212184.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
- Biological function summary
The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.
- Pathways
Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.
- Associated diseases and disorders
Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
11 product images
![Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--western-blot-img183710.jpg "Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)
This data was developed using Anti-Alpha-synuclein antibody [EPR20535] ab212184, the same antibody clone in a different buffer formulation
Blocking/Dilution buffer: 5% NFDM/TBST.
Human Alpha-synuclein recombinant protein contain aa1-140 with His-tag. Human Beta-synuclein recombinant protein contain aa1-134 with His-tag.
All lanes: Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866) at 1/1000 dilution
Lane 1: Human Alpha-synuclein recombinant protein at 0.01 µg
Lane 2: Human Beta-synuclein recombinant protein at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 18 kDa
Exposure time: 8s
![Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--western-blot-img175162.jpg "Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)
This data was developed using Anti-Alpha-synuclein antibody [EPR20535] ab212184, the same antibody clone in a different buffer formulation
Blocking/Dilution buffer: 5% NFDM/TBST.
The observed molecular weight is consistent with the literature (PMID: 11739566).
All lanes: Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866) at 1/1000 dilution
Lane 1: Human cerebellum lysate at 20 µg
Lane 2: Human brain lysate at 20 µg
Lane 3: Mouse brain lysate at 20 µg
Lane 4: Rat brain lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 18 kDa
Exposure time: 5s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111447.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)
Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling Alpha-synuclein with Anti-Alpha-synuclein antibody [EPR20535] ab212184 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Cytoplasmic staining on human cerebral cortex [PMID: 22112368].
Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Alpha-synuclein antibody [EPR20535] ab212184).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--western-blot-img166793.jpg "Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)
This data was developed using Anti-Alpha-synuclein antibody [EPR20535] ab212184, the same antibody clone in a different buffer formulation:
False colour image of Western blot: Anti-Alpha-synuclein antibody [EPR20535] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Alpha-synuclein antibody [EPR20535] ab212184 was shown to bind specifically to Alpha-synuclein. A band was observed at 16 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in SNCA knockout cell line Human SNCA (Alpha-synuclein) knockout U-87 MG cell line ab282333 (knockout cell lysate Human SNCA (Alpha-synuclein) knockout U-87 MG cell lysate ab283006). To generate this image, wild-type and SNCA knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866) at 1/1000 dilution
Lane 1: Wild-type U-87 MG cell lysate at 20 µg
Lane 2: SNCA knockout U-87 MG cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 16 kDa
![Immunohistochemistry (Frozen sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--immunohistochemistry-frozen-sections-img117859.jpg "Immunohistochemistry (Frozen sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
Immunohistochemistry (Frozen sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling Alpha-synuclein with Anti-Alpha-synuclein antibody [EPR20535] ab212184 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Cytoplasmic staining on mouse hippocampus (PMID: 22112368).
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Alpha-synuclein antibody [EPR20535] ab212184).
![Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--western-blot-img178244.jpg "Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)
This data was developed using Anti-Alpha-synuclein antibody [EPR20535] ab212184, the same antibody clone in a different buffer formulation:
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: SNCA (alpha Synuclein) knockout HAP1 whole cell lysate (20 μg)
Lane 3: Human brain whole tissue lysate (20 μg)
Lane 4: SH-SY5Y whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - Anti-Alpha-synuclein antibody [EPR20535] ab212184 observed at 14 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-Alpha-synuclein antibody [EPR20535] ab212184 was shown to specifically react with SNCA (alpha Synuclein) in wild type cells as signal was lost in SNCA (alpha Synuclein) knockout cells. Wild-type and SNCA (alpha Synuclein) knockout samples were subjected to SDS-PAGE. Anti-Alpha-synuclein antibody [EPR20535] ab212184 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)
Predicted band size: 14 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66442.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)
Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling Alpha-synuclein with Anti-Alpha-synuclein antibody [EPR20535] ab212184 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Cytoplasmic staining on human glioma [PMID: 22112368].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Alpha-synuclein antibody [EPR20535] ab212184).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--immunoprecipitation-img9378.jpg "Immunoprecipitation - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
Immunoprecipitation - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)
Alpha-synuclein was immunoprecipitated from 0.35 mg of mouse brain lysate with Anti-Alpha-synuclein antibody [EPR20535] ab212184 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using Anti-Alpha-synuclein antibody [EPR20535] ab212184 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse brainlysate 10ug (Input).
Lane 2: Anti-Alpha-synuclein antibody [EPR20535] ab212184 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Alpha-synuclein antibody [EPR20535] ab212184 in mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Alpha-synuclein antibody [EPR20535] ab212184).
All lanes: Immunoprecipitation - Anti-Alpha-synuclein antibody [EPR20535] (Anti-Alpha-synuclein antibody [EPR20535] ab212184)
Predicted band size: 14 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90420.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling Alpha-synuclein with Anti-Alpha-synuclein antibody [EPR20535] ab212184 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Cytoplasmic staining on mouse cerebral cortex [PMID: 22112368].
Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Alpha-synuclein antibody [EPR20535] ab212184).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100723.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling Alpha-synuclein with Anti-Alpha-synuclein antibody [EPR20535] ab212184 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Cytoplasmic staining on rat cerebral cortex [PMID: 22112368].
Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Alpha-synuclein antibody [EPR20535] ab212184).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119494.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Alpha-synuclein with Anti-Alpha-synuclein antibody [EPR20535] ab212184 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Negative control: No staining on human kidney. [PMID: 14997013].
Counter stained with Hematoxylin.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Alpha-synuclein antibody [EPR20535] ab212184).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--western-blot-img178244.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66442.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
![Immunoprecipitation - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--immunoprecipitation-img9378.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90420.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100723.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-epr20535-bsa-and-azide-free-ab225866--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119494.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [EPR20535] - BSA and Azide free (ab225866)")
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