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Anti-Alpha-synuclein antibody [LB 509] (ab27766) is a mouse monoclonal antibody detecting Alpha-synuclein in Western Blot, Flow Cytometry (Intra), Flow Cytometry. Suitable for Human, Rat,.



- Over 80 publications

- Trusted since 2006



Images

Western blot - Anti-Alpha-synuclein antibody [LB 509] (AB27766), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Alpha-synuclein antibody [LB 509] (AB27766), expandable thumbnail
  • Western blot - Anti-Alpha-synuclein antibody [LB 509] (AB27766), expandable thumbnail
  • Western blot - Anti-Alpha-synuclein antibody [LB 509] (AB27766), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WB
Human
Expected
Tested
Rat
Tested
Expected
Purified native protein - Human
Not recommended
Tested

Tested
Tested

Species
Rat
Dilution info
1 µg for 106 Cells
Notes

Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Purified native protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Purified native protein - Human
Dilution info
1/100.00000 - 1/1000.00000
Notes

-

Species
Human
Dilution info
1/100.00000 - 1/1000.00000
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

7 products for Alternative Product

Target data

Function

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

Recommended products

Anti-Alpha-synuclein antibody [LB 509] (ab27766) is a mouse monoclonal antibody detecting Alpha-synuclein in Western Blot, Flow Cytometry (Intra), Flow Cytometry. Suitable for Human, Rat,.



- Over 80 publications

- Trusted since 2006


Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
LB 509
Purification technique
Affinity purification
Epitope
ab27766 reacts with an epitope located in the region encoded by amino acids 115-122 of alpha-synuclein.
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Anti-Alpha-synuclein antibody [LB 509] (ab27766) is a mouse monoclonal antibody and is validated for use in Flow Cyt (Intra), Western Blot in human, mouse, rat samples.

Anti-Alpha-synuclein antibody [LB 509] (ab27766) has been cited over 81 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Alpha-synuclein antibody [LB 509] (Anti-SNCA antibody) (ab27766) has high sensitivity and specificity.

Anti-Alpha-synuclein antibody [LB 509] (ab27766) has 12 independent reviews from customers.

Anti-Alpha-synuclein antibody [LB 509] (ab27766) specifically detects Alpha-synuclein (UniProt ID: P37840; Molecular weight: 14kDa) and is sold in 100 µg selling sizes.

Synuclein Alpha (SNCA) is a protein primarily found in the brain, where it plays a crucial role in the regulation of synaptic vesicle trafficking and neurotransmitter release. It is a key component of Lewy bodies, which are pathological hallmarks of Parkinson's disease. Mutations and abnormal aggregation of SNCA are linked to several neurodegenerative disorders, including Parkinson's disease, dementia with Lewy bodies, and multiple system atrophy.

Alpha-synuclein is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals. The deposition of the abundant presynaptic brain protein alpha-synuclein as fibrillary aggregates in neurons or glial cells is a hallmark lesion in a subset of neurodegenerative disorders. These disorders include Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy, collectively referred to as synucleinopathies. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Associated diseases and disorders

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766), expandable thumbnail

    Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766)

    All lanes: Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766) at 1/1000 dilution

    All lanes: Western blot - Recombinant Human Alpha-synuclein protein (Recombinant Human Alpha-synuclein protein ab51189) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa

    Exposure time: 8min

  • Flow Cytometry (Intracellular) - Anti-Alpha-synuclein antibody [LB 509] (ab27766), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Alpha-synuclein antibody [LB 509] (ab27766)

    Overlay histogram showing PC12 (NGF differentiated) cells stained with ab27766 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab27766, 1μg/1x106) for 30 min at 22°C. The secondary antibody used was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 1/2000 dilution for 30 min at 22°C.

    Isotype control antibody (black line) was Mouse IgG1 [15-6E10A7] (Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW Blue laser (488nm) and 530/30 bandpass filter.

  • Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766), expandable thumbnail

    Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766)

    All lanes: Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766) at 5 µg/mL

    Lane 1: Western blot - Recombinant Human Alpha-synuclein protein (Recombinant Human Alpha-synuclein protein ab51189) at 10 µg

    Lane 2: Human brain hippocampus tissue lysate - total protein (ab30180) at 10 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa

    Observed band size: 16 kDa, 32 kDa

    Exposure time: 20min

  • Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766), expandable thumbnail

    Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766)

    All lanes: Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766) at 1/1000 dilution

    All lanes: Western blot - Recombinant Human Alpha-synuclein protein (Recombinant Human Alpha-synuclein protein ab51189) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 5000 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa

    Exposure time: 8min

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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