AB27766

Anti-Alpha-synuclein antibody [LB 509]

Name: Anti-alpha-synuclein [LB 509] antibody - mouse monoclonal (ab27766) | Abcam
Brand: Abcam Limited
SKU: AB27766
Price: 445 USD
Availability: InStock
Rating: 4 (13 reviews)

(13 Reviews)

(100 Publications)

Anti-Alpha-synuclein antibody [LB 509] (ab27766) is a mouse monoclonal antibody detecting Alpha-synuclein in Western Blot, Flow Cytometry (Intra). Suitable for Human, Rat,.

- Over 80 publications
- Trusted since 2006

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

4 Images

Unknown

Western blot - Anti-Alpha-synuclein antibody [LB 509] (AB27766)

All lanes:

Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766) at 1/1000 dilution

All lanes:

Western blot - Recombinant Human Alpha-synuclein protein (Tag Free) (<a href='/en-us/products/proteins-peptides/recombinant-human-alpha-synuclein-protein-ab51189'>ab51189</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 14 kDa

true

Exposure time: 8min

Flow Cytometry (Intracellular) - Anti-Alpha-synuclein antibody [LB 509] (AB27766)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Alpha-synuclein antibody [LB 509] (AB27766)

Overlay histogram showing PC12 (NGF differentiated) cells stained with ab27766 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab27766, 1μg/1x10⁶) for 30 min at 22°C. The secondary antibody used was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.

Isotype control antibody (black line) was Mouse IgG1 [15-6E10A7] (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50mW Blue laser (488nm) and 530/30 bandpass filter.

Lab

Western blot - Anti-Alpha-synuclein antibody [LB 509] (AB27766)

All lanes:

Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766) at 5 µg/mL

Lane 1:

Western blot - Recombinant Human Alpha-synuclein protein (Tag Free) (<a href='/en-us/products/proteins-peptides/recombinant-human-alpha-synuclein-protein-ab51189'>ab51189</a>) at 10 µg

Lane 2:

Human brain hippocampus tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-hippocampus-tissue-lysate-total-protein-ab30180'>ab30180</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 14 kDa

Observed band size: 16 kDa,32 kDa

true

Exposure time: 20min

Unknown

Western blot - Anti-Alpha-synuclein antibody [LB 509] (AB27766)

All lanes:

Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766) at 1/1000 dilution

All lanes:

Western blot - Recombinant Human Alpha-synuclein protein (Tag Free) (<a href='/en-us/products/proteins-peptides/recombinant-human-alpha-synuclein-protein-ab51189'>ab51189</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 5000 µg

Predicted band size: 14 kDa

true

Exposure time: 8min

Carrier free

Anti-Alpha-synuclein antibody [LB 509] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LB 509

Isotype

IgG1

Carrier free

Reacts with

Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

ab27766 reacts with an epitope located in the region encoded by amino acids 115-122 of alpha-synuclein.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Purified native protein - Human": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "" } } }

style

left-column

Product details

Anti-Alpha-synuclein antibody [LB 509] (ab27766) is a mouse monoclonal antibody and is validated for use in Flow Cyt (Intra), Western Blot in human, mouse, rat samples.

Anti-Alpha-synuclein antibody [LB 509] (ab27766) has been cited over 81 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Alpha-synuclein antibody [LB 509] (Anti-SNCA antibody) (ab27766) has high sensitivity and specificity.

Anti-Alpha-synuclein antibody [LB 509] (ab27766) has 12 independent reviews from customers.

Anti-Alpha-synuclein antibody [LB 509] (ab27766) specifically detects Alpha-synuclein (UniProt ID: P37840; Molecular weight: 14kDa) and is sold in 100 µg selling sizes.

Synuclein Alpha (SNCA) is a protein primarily found in the brain, where it plays a crucial role in the regulation of synaptic vesicle trafficking and neurotransmitter release. It is a key component of Lewy bodies, which are pathological hallmarks of Parkinson's disease. Mutations and abnormal aggregation of SNCA are linked to several neurodegenerative disorders, including Parkinson's disease, dementia with Lewy bodies, and multiple system atrophy.

Alpha-synuclein is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals. The deposition of the abundant presynaptic brain protein alpha-synuclein as fibrillary aggregates in neurons or glial cells is a hallmark lesion in a subset of neurodegenerative disorders. These disorders include Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy, collectively referred to as synucleinopathies. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed : 20798282, PubMed : 26442590, PubMed : 28288128, PubMed : 30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed : 28288128, PubMed : 30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed : 30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed : 20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed : 26442590).

See full target information SNCA

style

left-column

Publications (100)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 13: PubMed40427069

2025

Renalase Overexpression-Mediated Excessive Metabolism of Peripheral Dopamine, DOPAL Accumulation, and α-Synuclein Aggregation in Baroreflex Afferents Contribute to Neuronal Degeneration and Autonomic Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Xiong,Yin-Zhi Xu,Yan Zhang,Hong-Fei Zhang,Tian-Min Dou,Xing-Yu Li,Zhao-Yuan Xu,Chang-Peng Cui,Xue-Lian Li,Bai-Yan Li

Molecular neurodegeneration 20:26 PubMed40038767

2025

Age-dependent progression from clearance to vulnerability in the early response of periventricular microglia to α-synuclein toxic species.

Applications

Unspecified application

Species

Unspecified reactive species

Mª Salomé Sirerol-Piquer,Ana Perez-Villalba,Pere Duart-Abadia,Germán Belenguer,Ulises Gómez-Pinedo,Laura Blasco-Chamarro,Pau Carrillo-Barberà,Azucena Pérez-Cañamás,Victoria Navarro-Garrido,Benjamin Dehay,Miquel Vila,Javier Vitorica,Francisco Pérez-Sánchez,Isabel Fariñas

Communications biology 8:288 PubMed39987244

2025

Female mice exhibit resistance to disease progression despite early pathology in a transgenic mouse model inoculated with alpha-synuclein fibrils.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Tullo,Janice Sung Hyun Park,Daniel Gallino,Megan Park,Kristie Mar,Vladislav Novikov,Rodrigo Sandoval Contreras,Raihaan Patel,Esther Del Cid-Pellitero,Edward A Fon,Wen Luo,Irina Shlaifer,Thomas M Durcan,Marco A M Prado,Vania F Prado,Gabriel A Devenyi,M Mallar Chakravarty

NPJ Parkinson's disease 10:227 PubMed39613827

2024

MJF-14 proximity ligation assay detects early non-inclusion alpha-synuclein pathology with enhanced specificity and sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Nanna Møller Jensen,YuHong Fu,Cristine Betzer,Hongyun Li,Sara Elfarrash,Ali H Shaib,Donatus Krah,Zagorka Vitic,Lasse Reimer,Hjalte Gram,Vladimir Buchman,Mark Denham,Silvio O Rizzoli,Glenda M Halliday,Poul Henning Jensen

NPJ Parkinson's disease 10:206 PubMed39463404

2024

Structural basis of epitope recognition by anti-alpha-synuclein antibodies MJFR14-6-4-2.

Applications

Unspecified application

Species

Unspecified reactive species

Ilva Liekniņa,Lasse Reimer,Teodors Panteļejevs,Alons Lends,Kristaps Jaudzems,Aadil El-Turabi,Hjalte Gram,Anissa Hammi,Poul Henning Jensen,Kaspars Tārs

Immunity & ageing : I & A 21:47 PubMed38997709

2024

Nuclear alpha-synuclein accelerates cell senescence and neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Tingfu Du,Guoxiang Li,Qinglan Zong,Haiyu Luo,Yue Pan,Kaili Ma

Brain : a journal of neurology 147:3727-3741 PubMed38696728

2024

Distinct ultrastructural phenotypes of glial and neuronal alpha-synuclein inclusions in multiple system atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Carolin Böing,Marta Di Fabrizio,Domenic Burger,John G J M Bol,Evelien Huisman,Annemieke J M Rozemuller,Wilma D J van de Berg,Henning Stahlberg,Amanda J Lewis

Cell death discovery 10:167 PubMed38589400

2024

Thrombomodulin reduces α-synuclein generation and ameliorates neuropathology in a mouse model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Yun Niu,Xi-Xiu Xie,Hou-Zhen Tuo,Cui-Ping Lv,Ya-Ru Huang,Jie Zhu,Shi-Yu Liang,Xiao-Yu Du,Cheng-Gang Yang,Sheng-Jie Hou,Xiao-Ying Sun,Ling-Jie Li,Fang Cui,Qi-Xin Huang,Ying-Bo Jia,Yu-Jiong Wang,Rui-Tian Liu

Scientific reports 14:6239 PubMed38486089

2024

Solubility of α-synuclein species in the L62 mouse model of synucleinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Karima Schwab,Mandy Magbagbeolu,Franz Theuring,Charles R Harrington,Claude M Wischik,Gernot Riedel

NPJ Parkinson's disease 10:37 PubMed38368444

2024

Angiotensin type 1 receptor activation promotes neuronal and glial alpha-synuclein aggregation and transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Lage,Ana I Rodriguez-Perez,Begoña Villar-Cheda,Jose L Labandeira-Garcia,Antonio Dominguez-Meijide

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alpha-synuclein antibody [LB 509]

Western blot - Anti-Alpha-synuclein antibody [LB 509] (AB27766)

Flow Cytometry (Intracellular) - Anti-Alpha-synuclein antibody [LB 509] (AB27766)

Western blot - Anti-Alpha-synuclein antibody [LB 509] (AB27766)

Western blot - Anti-Alpha-synuclein antibody [LB 509] (AB27766)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (100)

Product promise