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AB259611

Anti-Alpha-synuclein antibody [MJFR-14-6-4-2] - BSA and Azide free (Capture)

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Anti-Alpha-synuclein antibody [MJFR-14-6-4-2] - BSA and Azide free (Capture) (ab259611) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Alpha-synuclein in ELISA. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

1 Images
Sandwich ELISA - Anti-Alpha-synuclein antibody [MJFR-14-6-4-2] - BSA and Azide free (Capture) (AB259611)
  • sELISA

Lab

Sandwich ELISA - Anti-Alpha-synuclein antibody [MJFR-14-6-4-2] - BSA and Azide free (Capture) (AB259611)

Sandwich ELISA - Recombinant human Alpha-Synuclein protein filament standard curve.

Background subtracted standard curve using Anti-Alpha-synuclein antibody [MJFR-14-6-4-2] - BSA and Azide free (Capture), Anti-Alpha-synuclein antibody [MJFR1] - BSA and Azide free (Detector), and Recombinant human Alpha-Synuclein protein filament (ab254309) in sandwich ELISA.

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2]

  • Biotin

    Biotin Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific

  • Unconjugated

    Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific

  • Carrier free

    Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MJFR-14-6-4-2

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Alpha-synuclein antibody [MJFR-14-6-4-2] - BSA and Azide free (Capture) (ab259611) is a rabbit recombinant monoclonal antibody and is validated for use in ELISA in Human samples.

Other related products
We have a range of other formats of antibody clone [MJFR-14-6-4-2] also available for your convenience: ab209538, Carrier free - ab214033, Alexa Fluor® 488 - ab216124, Alexa Fluor® 647 - ab216309, Biotin - ab227047, Carrier free - ab259611, Alexa Fluor® 594 - ab283628, Alexa Fluor® 555 - ab283737

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.
See full target information SNCA
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Product promise

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