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Chicken Monoclonal Alpha-synuclein antibody. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (AB320839), expandable thumbnail
  • Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (AB320839), expandable thumbnail
  • Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (AB320839), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (AB320839), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (AB320839), expandable thumbnail

Key facts

Isotype
IgY
Host species
Chicken
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PFlow Cyt (Intra)IPICC/IF
Human
Tested
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

Chicken Monoclonal Alpha-synuclein antibody. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgY
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
MJFR1
Purification technique
Ion exchange chromatography Thiophilic Resin
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This chicken antibody has been engineered from a RabMAb parent antibody (Anti-Alpha-synuclein antibody [MJFR1] ab138501). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (ab320839), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (ab320839)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Alpha-synuclein with ab320839 at 1/5000 (0.211 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human liver.

    The section was incubated with ab320839 for 10 mins at room temperature, followed by anti-Chicken IgY antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (ab320839), expandable thumbnail

    Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (ab320839)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: liver and kidney ( (PMID:14997013).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (ab320839) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 20 µg

    Lane 2: Human liver tissue lysate at 20 µg

    Lane 3: Human kidney tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) preadsorbed (Goat Anti-Chicken IgY H&L (HRP) preadsorbed ab7118) at 1/2000 dilution

    Observed band size: 18 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (ab320839), expandable thumbnail

    Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (ab320839)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, ab320839 was shown to bind specifically to SNCA. Target of interest was observed at 18kDa in wild-type HAP1 cell lysates (lane 1) with no signal observed at this size in SNCA knockout cell line (lane 2).

    The identity of the bands above target band are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (ab320839) at 1/1000 dilution

    Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

    Lane 2: SNCA knockout HAP1 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) preadsorbed (Goat Anti-Chicken IgY H&L (HRP) preadsorbed ab7118) at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 18 kDa, 36 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (ab320839), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (ab320839)

    Immunohistochemical analysis of paraffin-embedded (A) Parental HAP1 (human chronic myelogenous leukemia near-haploid cell) cell pellet (B) SNCA knockout HAP1 (HO010074) cell pellet tissue labeling Alpha-synuclein with ab320839 at 1/1000 (1.055 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Cytoplasmic staining on (A) Parental HAP1 cell pellet, no staining on (B) SNCA knockout HAP1 (HO010074) cell pellet.

    The section was incubated with ab320839 for 10 mins at room temperature, followed by anti-Chicken IgY (Rabbit Anti-Chicken IgY H&L ab97136) antibody for 8 mins during the LeicaDS9800 kit staining procedure.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (ab320839), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Chicken IgY (Chimeric) (ab320839)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Alpha-synuclein with ab320839 at 1/5000 (0.211 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human cerebrum.

    The section was incubated with ab320839 for 10 mins at room temperature, followed by anti-Chicken IgY antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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