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Knockout Tested Mouse Monoclonal Alpha-synuclein antibody. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (AB309157), expandable thumbnail
  • Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (AB309157), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (AB309157), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (AB309157), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (AB309157), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Tested
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500 - 1/50000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

Target data

Function

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

Recommended products

Knockout Tested Mouse Monoclonal Alpha-synuclein antibody. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
MJFR1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

ab309157 is a mouse monoclonal chimeric antibody.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Associated diseases and disorders

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (ab309157), expandable thumbnail

    Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (ab309157)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, ab309157 was shown to bind specifically to Alpha-synuclein.  Target of interest was observed at 18 kDa in wild-type HAP1 cell lysates (lane 1) with no signal observed at this size in Alpha-synuclein knockout cell line (lane 2).

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time: 180 seconds.

    All lanes: Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (ab309157) at 1/1000 dilution

    Lane 1: Parental HAP1 whole cell lysate at 20 µg

    Lane 2: Alpha-synuclein knockout HAP1 whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Observed band size: 18 kDa

    Exposure time: 180s

  • Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (ab309157), expandable thumbnail

    Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (ab309157)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Kidney and heart. (PMID: 14997013).

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time: 37 seconds.

    All lanes: Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (ab309157) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 10 µg

    Lane 2: Human heart tissue lysate at 10 µg

    Lane 3: Human kidney tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (Goat Anti-Mouse IgG Fc (HRP) preadsorbed ab98717) at 1/20000 dilution

    Observed band size: 18 kDa

    Exposure time: 37s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (ab309157), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (ab309157)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Alpha-synuclein with ab309157 at 1/50000 (0.017 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Cytoplasmic staining on human cerebrum.

    The section was incubated with ab309157 for 10 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (ab309157), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (ab309157)

    Immunohistochemical analysis of paraffin-embedded cells labeling Alpha-synuclein with ab309157 at 1/500 (1.7 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Cytoplasmic staining on (A) Parental HAP1 cell pellet, no staining on (B) SNCA knockout HAP1 (HO010074) cell pellet.

    The section was incubated with ab309157 for 10 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (ab309157), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (ab309157)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Alpha-synuclein with ab309157 at 1/50000 (0.017 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on human kidney.

    The section was incubated with ab309157 for 10 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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