Mouse Monoclonal Alpha-synuclein antibody. Suitable for ICC and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SNCA.
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![Immunocytochemistry - Anti-Alpha-synuclein antibody [syn204] (AB3309), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-syn204-ab3309--immunocytochemistry-img29813.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-Alpha-synuclein antibody [syn204] (AB3309)")
Publications
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- Cells 11:2022ATPase Thorase Deficiency Causes α-Synucleinopathy and Parkinson's Disease-like Behavior.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFei Gao,Han Zhang,Jia Yang,Menghua Cai,Qi Yang,Huaishan Wang,Yi Xu,Hui Chen,Yu Hu,Wei He,Jianmin ZhangPubMed 36230952
- Frontiers in neuroscience 14:5754592020Neuroprotective and Neurorestorative Effects of Extract in the MPTP/MPP-Induced Mouse and Cellular Models of Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKunwadee Noonong et. al.PubMed 33408606
- Neural regeneration research 15:361-3682019Ethanol extract from ameliorates dopaminergic neuronal cell death in transgenic mice expressing mutant A53T human alpha-synuclein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun Jin Park,Ting Ting Zhao,Seung Hwan Kim,Chong Kil Lee,Bang Yeon Hwang,Kyung Eun Lee,Myung Koo LeePubMed 31552910
- eLife 8:2019An engineered monomer binding-protein for α-synuclein efficiently inhibits the proliferation of amyloid fibrils.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmil Dandanell Agerschou et. al.PubMed 31389332
- Biochimica et biophysica acta. Molecular basis of 1864:3714-37252018C-terminal truncation exacerbates the aggregation and cytotoxicity of α-Synuclein: A vicious cycle in Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Ma et. al.PubMed 30290273
- Neurochemistry international 120:172-1812018Yeast red pigment modifies cloned human α-synuclein pathogenesis in Parkinson disease models in Saccharomyces cerevisiae and Drosophila melanogaster.Applications:Unspecified applicationReactive species:Unspecified reactive speciesO V Nevzglyadova et. al.PubMed 30099122
- JAMA neurology 74:163-1722016Development of a Biochemical Diagnosis of Parkinson Disease by Detection of α-Synuclein Misfolded Aggregates in Cerebrospinal Fluid.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammad Shahnawaz,Takahiko Tokuda,Masaaki Waragai,Nicolas Mendez,Ryotaro Ishii,Claudia Trenkwalder,Brit Mollenhauer,Claudio SotoPubMed 27918765
- Proceedings of the National Academy of Sciences of the United States of America 113:9593-82016Reducing C-terminal truncation mitigates synucleinopathy and neurodegeneration in a transgenic model of multiple system atrophy.Applications:SDS-PAGEReactive species:Unspecified reactive speciesFares Bassil,Pierre-Olivier Fernagut,Erwan Bezard,Alain Pruvost,Thierry Leste-Lasserre,Quyen Q Hoang,Dagmar Ringe,Gregory A Petsko,Wassilios G MeissnerPubMed 27482103
- Nature protocols 9:2135-462014Addition of exogenous α-synuclein preformed fibrils to primary neuronal cultures to seed recruitment of endogenous α-synuclein to Lewy body and Lewy neurite-like aggregates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura A Volpicelli-Daley et. al.PubMed 25122523
- Neuropathology and applied neurobiology 32:351-62006Comparative study of commercially available anti-alpha-synuclein antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesE Croisier,D Elfant MRes,M Deprez,K Goldring,D T Dexter,R K B Pearce,M B Graeber,F RoncaroliPubMed 16640654
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human SNCA. Database link P37840
Reactivity data
Select an application| ICC | |
|---|---|
| Human | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
Associated Products
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Target data
Function
The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor
Recommended products
Mouse Monoclonal Alpha-synuclein antibody. Suitable for ICC and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SNCA.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human SNCA. Database link P37840
- Clone number
- syn204
- Purification technique
- Affinity purification Protein G
- Specificity
Due to sequence homology ab3309 might react with Beta synuclein
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
- Biological function summary
The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.
- Pathways
Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.
- Associated diseases and disorders
Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunocytochemistry - Anti-Alpha-synuclein antibody [syn204] (ab3309), expandable thumbnail](https://content.abcam.com/products/images/alpha-synuclein-antibody-syn204-ab3309--immunocytochemistry-img29813.jpg "Immunocytochemistry - Anti-Alpha-synuclein antibody [syn204] (ab3309)")
Immunocytochemistry - Anti-Alpha-synuclein antibody [syn204] (ab3309)
ab3309 staining Alpha-Synuclein in wild-type Hap1 cells (top panel) and SNCA knockout Hap1 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab3309 at 10μg/ml concentration and Anti-beta Tubulin antibody - Loading Control ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
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