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Rabbit Recombinant Monoclonal Alpha-synuclein antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Immunocytochemistry - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (AB290644), expandable thumbnail
  • Western blot - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (AB290644), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (AB290644), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (AB290644), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (AB290644), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBFlow Cyt (Intra)IHC-P
Human
Tested
Not recommended
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Target data

Function

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.

Additional Targets

Alpha-synuclein

Alternative names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

Recommended products

Rabbit Recombinant Monoclonal Alpha-synuclein antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25117-81
Purification technique
Affinity purification Protein A
Specificity

Alpha-synuclein, isoform 98 and 126.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha-synuclein isoform 98 and 126 also known as SNCA plays a mechanical role in neurons. This protein is an important part of the nerve cell cytoplasm distributed mainly in presynaptic terminals of the brain. With a molecular weight of about 14 kDa SNCA functions in both soluble and membrane-bound forms. Expression of alpha-synuclein occurs in the central nervous system but it is seen in some peripheral tissues too.

Biological function summary

The protein contributes to the regulation of synaptic vesicle trafficking and neurotransmitter release. As part of a complex alpha-synuclein interacts with a variety of other synaptic proteins affecting vesicle mobility and ensuring efficient synaptic transmission. It influences neuronal plasticity and survival supporting nervous system function. Though still under investigation these functions indicate a regulatory role in maintaining neural communication.

Pathways

One can see the involvement of alpha-synuclein in the dopamine and calcium signaling pathways. These pathways impact synaptic transmission with overlaps including proteins like LRRK2 and parkin. Alpha-synuclein's regulation of cellular transport processes connects these pathways impacting their overall function by modulating synaptic vesicle dynamics.

Associated diseases and disorders

Alpha-synuclein is linked to Parkinson's Disease and Lewy Body Dementia. These neurodegenerative disorders show abnormal accumulation of the protein. Interactions between SNCA and others such as tau protein and amyloid precursor protein lead to pathological changes. Understanding these interactions provides insight into how alpha-synuclein contributes to disease mechanisms making it an important focus for therapeutic research.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunocytochemistry - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (ab290644), expandable thumbnail

    Immunocytochemistry - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (ab290644)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized 293T (human embryonic kidney epithelial cell) cells lebelling Alpha-synuclein, isoform 98 and 126 with ab290644 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HEK-293T cells transfected with a human alpha-synuclein isoform SNCA98 and SNCA126 expression vector containing a myc tag is observed. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Western blot - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (ab290644), expandable thumbnail

    Western blot - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (ab290644)

    Blocking and diluting buffer and concentration was 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Western blot - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (ab290644) at 1/1000 dilution

    Lane 1: HEK-293T (human embryonic kidney) cells transfected with a human SNCA140 expression vector containing a myc-His tag whole cell lysate at 20 µg

    Lane 2: HEK-293T cells transfected with a human SNCA126 expression vector containing a myc-His tag whole cell lysate at 20 µg

    Lane 3: HEK-293T cells transfected with a human SNCA112 expression vector containing a myc-His tag whole cell lysate at 20 µg

    Lane 4: HEK-293T cells transfected with a human SNCA98 expression vector containing a myc-His tag whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (ab290644), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (ab290644)

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells tissue labelling Alpha-synuclein, isoform 98 and 126 with ab290644 at 1/100 (4.81 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on (A) HEK-293T cells transfected with a SNCA98 expression vector containing a his tag and (B) HEK-293T cells transfected with a SNCA126 expression vector containing a his tag. No staining on (C) HEK-293T cells transfected with a SNCA140 expression vector containing a his tag, (D) HEK-293T cells transfected with a SNCA112 expression vector containing a his tag and (E) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab290644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (ab290644), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (ab290644)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized 293T (human embryonic kidney epithelial cell) cells lebelling Alpha-synuclein, isoform 98 and 126 with ab290644 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor=® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing no staining in HEK-293T cells transfected with a human alpha-synuclein isoform SNCA140 or SNCA112 expression vector containing a myc tag is observed. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (ab290644), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (ab290644)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with a human Alpha-synuclein, isoform 140 (Left) or Alpha-synuclein, isoform 112 (Right) expression vector containing a myc-His tag cells labelling Alpha-synuclein, isoform 98 and 126 with ab290644 at 1/500 dilution/ Left and Right (Red) compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. No cross-reactivity with Alpha-synuclein, isoform 140 and isoform 112.

  • Flow Cytometry (Intracellular) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (ab290644), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (ab290644)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with a human Alpha-synuclein, isoform 126 (Left) or Alpha-synuclein, isoform 98 (Right) expression vector containing a myc-His tag cells labelling Alpha-synuclein, isoform 98 and 126 with ab290644 at 1/500 dilution/ Left and Right compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

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