AB290655

Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal Alpha-synuclein antibody. Carrier free. Suitable for ICC, WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (AB290655)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (AB290655)

This data was developed using ab290644, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized 293T (human embryonic kidney epithelial cell) cells lebelling Alpha-synuclein, isoform 98 and 126 with ab290644 at 1/2000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor=® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing no staining in HEK-293T cells transfected with a human alpha-synuclein isoform SNCA140 or SNCA112 expression vector containing a myc tag is observed. Myc-Tag Mouse mAb (Alexa Fluor^® 647) was used to counterstain tubulin at 1/100 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (AB290655)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (AB290655)

This data was developed using ab290644, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with a human Alpha-synuclein, isoform 140 (Left) or Alpha-synuclein, isoform 112 (Right) expression vector containing a myc-His tag cells labelling Alpha-synuclein, isoform 98 and 126 with ab290644 at 1/500 dilution/ Left and Right compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. No cross-reactivity with Alpha-synuclein, isoform 140 and isoform 112.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (AB290655)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (AB290655)

This data was developed using ab290644, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells tissue labelling Alpha-synuclein, isoform 98 and 126 with ab290644 at 1/100 (4.81 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on (A) HEK-293T cells transfected with a SNCA98 expression vector containing a his tag and (B) HEK-293T cells transfected with a SNCA126 expression vector containing a his tag. No staining on (C) HEK-293T cells transfected with a SNCA140 expression vector containing a his tag, (D) HEK-293T cells transfected with a SNCA112 expression vector containing a his tag and (E) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab290644 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (AB290655)

This data was developed using ab290644, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with a human Alpha-synuclein, isoform 126 (Left) or Alpha-synuclein, isoform 98 (Right) expression vector containing a myc-His tag cells labelling Alpha-synuclein, isoform 98 and 126 with ab290644 at 1/500 dilution. Left and Right compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Immunocytochemistry - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (AB290655)

This data was developed using ab290644, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized 293T (human embryonic kidney epithelial cell) cells lebelling Alpha-synuclein, isoform 98 and 126 with ab290644 at 1/2000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^®488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HEK-293T cells transfected with a human alpha-synuclein isoform SNCA98 and SNCA126 expression vector containing a myc tag is observed. Myc-Tag Mouse mAb (Alexa Fluor^® 647) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution.

Unknown

Western blot - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (AB290655)

This data was developed using ab290744, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration was 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] (<a href='/en-us/products/primary-antibodies/alpha-synuclein-isoform-98-and-126-antibody-epr25117-81-ab290644'>ab290644</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney) cells transfected with a human SNCA140 expression vector containing a myc-His tag whole cell lysate at 20 µg

Lane 2:

HEK-293T cells transfected with a human SNCA126 expression vector containing a myc-His tag whole cell lysate at 20 µg

Lane 3:

HEK-293T cells transfected with a human SNCA112 expression vector containing a myc-His tag whole cell lysate at 20 µg

Lane 4:

HEK-293T cells transfected with a human SNCA98 expression vector containing a myc-His tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 3min

Unconjugated

Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25117-81

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra), ICC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Alpha-synuclein, isoform 98 and 126.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein isoform 98 and 126 also known as SNCA plays a mechanical role in neurons. This protein is an important part of the nerve cell cytoplasm distributed mainly in presynaptic terminals of the brain. With a molecular weight of about 14 kDa SNCA functions in both soluble and membrane-bound forms. Expression of alpha-synuclein occurs in the central nervous system but it is seen in some peripheral tissues too.

Biological function summary

The protein contributes to the regulation of synaptic vesicle trafficking and neurotransmitter release. As part of a complex alpha-synuclein interacts with a variety of other synaptic proteins affecting vesicle mobility and ensuring efficient synaptic transmission. It influences neuronal plasticity and survival supporting nervous system function. Though still under investigation these functions indicate a regulatory role in maintaining neural communication.

Pathways

One can see the involvement of alpha-synuclein in the dopamine and calcium signaling pathways. These pathways impact synaptic transmission with overlaps including proteins like LRRK2 and parkin. Alpha-synuclein's regulation of cellular transport processes connects these pathways impacting their overall function by modulating synaptic vesicle dynamics.

Alpha-synuclein is linked to Parkinson's Disease and Lewy Body Dementia. These neurodegenerative disorders show abnormal accumulation of the protein. Interactions between SNCA and others such as tau protein and amyloid precursor protein lead to pathological changes. Understanding these interactions provides insight into how alpha-synuclein contributes to disease mechanisms making it an important focus for therapeutic research.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.

See full target information SNCA

Additional targets

Alpha-synuclein

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (AB290655)

Flow Cytometry (Intracellular) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (AB290655)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (AB290655)

Flow Cytometry (Intracellular) - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (AB290655)

Immunocytochemistry - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (AB290655)

Western blot - Anti-Alpha-synuclein, isoform 98 and 126 antibody [EPR25117-81] - BSA and Azide free (AB290655)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise