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Rabbit Recombinant Monoclonal Alpha-synuclein nitrated Y133 antibody. Suitable for I-ELISA and reacts with Synthetic peptide - Human samples.

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Images

ELISA - Anti-Alpha-synuclein (Nitration Tyr133) antibody [EPR27843-41] (AB323900), expandable thumbnail
  • Indirect ELISA - Anti-Alpha-synuclein (Nitration Tyr133) antibody [EPR27843-41] (AB323900), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
I-ELISAIHC-FrIHC-PWB
Human
Predicted
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Synthetic peptide - Human
Dilution info
62.5 ng/mL
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Synthetic peptide - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Synthetic peptide - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Synthetic peptide - Human, Mouse
Dilution info
-
Notes

-

Target data

Function

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

NACP, PARK1, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

Rabbit Recombinant Monoclonal Alpha-synuclein nitrated Y133 antibody. Suitable for I-ELISA and reacts with Synthetic peptide - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27843-41
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • ELISA - Anti-Alpha-synuclein (Nitration Tyr133) antibody [EPR27843-41] (ab323900), expandable thumbnail

    ELISA - Anti-Alpha-synuclein (Nitration Tyr133) antibody [EPR27843-41] (ab323900)

    Indirect ELISA analysis of ab323900 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution dilution.

    Antigen: Alpha-synuclein peptide.

    Antigen concentration: 1000 ng/ml

    The data was kindly provided by a collaborator.

    TC = Temporal Cortex

    DMV = Dorsal motor nucleus of the vagus nerve

    SN = Substantia nigra

    PD= Parkinson's disease

    CTRL= control

    The clone detected an increase of signal in PD DMV and SN brain lysate.

  • Indirect ELISA - Anti-Alpha-synuclein (Nitration Tyr133) antibody [EPR27843-41] (ab323900), expandable thumbnail

    Indirect ELISA - Anti-Alpha-synuclein (Nitration Tyr133) antibody [EPR27843-41] (ab323900)

    Indirect ELISA analysis of ab323900 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution dilution.

    Antigen: Alpha-synuclein peptide.

    Antigen concentration: 1000 ng/ml

    This antibody does not cross-react with Alpha-synuclein nitrated at Tyr125 or Tyr136; dinitrosylated at Tyr125 and Tyr133; at Tyr118 and Tyr126; at Tyr125 and Tyr126; at Tyr133 and Tyr126; trinitrosylated at Tyr125, Tyr133, and Tyr136; or diphosphorylated at Tyr125 and Tyr133.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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