Rabbit Polyclonal Alpha-synuclein phospho S129 antibody. Suitable for WB and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Rat Snca phospho S129.
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- Scientific reports 7:163862017Alpha-synuclein facilitates to form short unconventional microtubules that have a unique function in the axonal transport.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiori Toba et. al.PubMed 29180624
- Journal of neurochemistry 122:812-222012Potential role of α-synuclein in neurodegeneration: studies in a rat animal model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeorge Stoica et. al.PubMed 22639889
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Rat Snca phospho S129. The exact immunogen used to generate this antibody is proprietary information. Database link P37377
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Reactivity data
Select an application| WB | |
|---|---|
| Mouse | Predicted |
| Rat | Tested |
| Cow | Predicted |
| Dog | Predicted |
| Primates | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Dog, Primates | Dilution info - | Notes - |
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Target data
Function
Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (By similarity). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (By similarity). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (By similarity). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (By similarity). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (By similarity). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (By similarity).
Alternative names
Alpha-synuclein, Snca
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Rabbit Polyclonal Alpha-synuclein phospho S129 antibody. Suitable for WB and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Rat Snca phospho S129.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Rat Snca phospho S129. The exact immunogen used to generate this antibody is proprietary information. Database link P37377
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody is specific for the 15 kDa alpha Synuclein protein phosphorylated at serine 129. Immunolabelling is blocked by the phosphopeptide used as an antigen but not by the corresponding dephosphopeptide.
- Concentration
- Purification notes
Prepared from rabbit serum by affinity purification via sequential chromatography on phosph- and dephosphopeptide affinity columns.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
- Biological function summary
The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.
- Pathways
Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.
- Associated diseases and disorders
Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Alpha-synuclein (phospho S129) antibody (ab42906)
Western blot of rat cortex lysate showing specific labelling of the ~15kDa alpha Synuclein protein phosphorylated at serine 129, using ab42906 at a 1/1000 dilution.
Immunolabeling is blocked by the phosphopeptide (peptide) used as immunogen.All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody (ab42906) at 1/1000 dilution
Lane 1: Rat cortex lysate at 20 µg
Lane 2: Rat cortex lysate with immunogenic peptide 1ng
Predicted band size: 14 kDa
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