Anti-Alpha-synuclein (phospho S129) antibody (ab59264) is a rabbit polyclonal antibody that is used to detect Alpha-synuclein in Western Blot, IHC-P. Suitable for Human, Mouse samples.
- Over 60 publications
- Trusted since 2007
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- Nature communications 14:75292023Brain-to-gut trafficking of alpha-synuclein by CD11c cells in a mouse model of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRhonda L McFleder,Anastasiia Makhotkina,Janos Groh,Ursula Keber,Fabian Imdahl,Josefina Peña Mosca,Alina Peteranderl,Jingjing Wu,Sawako Tabuchi,Jan Hoffmann,Ann-Kathrin Karl,Axel Pagenstecher,Jörg Vogel,Andreas Beilhack,James B Koprich,Jonathan M Brotchie,Antoine-Emmanuel Saliba,Jens Volkmann,Chi Wang IpPubMed 37981650
- Frontiers in aging neuroscience 15:11174912023Uric acid regulates α-synuclein transmission in Parkinsonian models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Jin Shin,Yeon Ju Kim,Ji Eun Lee,Yi Seul Kim,Jung Wook Lee,HyeonJeong Kim,Jin Young Shin,Phil Hyu LeePubMed 37711993
- Biomedicines 11:2023UBL3 Interacts with Alpha-Synuclein in Cells and the Interaction Is Downregulated by the EGFR Pathway Inhibitor Osimertinib.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Chen,Md Mahmudul Hasan,Hengsen Zhang,Qing Zhai,A S M Waliullah,Yashuang Ping,Chi Zhang,Soho Oyama,Mst Afsana Mimi,Yuna Tomochika,Yu Nagashima,Tomohiko Nakamura,Tomoaki Kahyo,Kenji Ogawa,Daita Kaneda,Minoru Yoshida,Mitsutoshi SetouPubMed 37371780
- Cell death & disease 13:10482022Neuronal haemoglobin induces loss of dopaminergic neurons in mouse Substantia nigra, cognitive deficits and cleavage of endogenous α-synuclein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiara Santulli,Carlotta Bon,Elena De Cecco,Marta Codrich,Joanna Narkiewicz,Pietro Parisse,Fabio Perissinotto,Claudio Santoro,Francesca Persichetti,Giuseppe Legname,Stefano Espinoza,Stefano GustincichPubMed 36526614
- Methods in molecular biology (Clifton, N.J.) 2551:357-3782022Propagation of Distinct α-Synuclein Strains Within Human Reconstructed Neuronal Network and Associated Neuronal Dysfunctions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimona Gribaudo,Luc Bousset,Josquin Courte,Ronald Melki,Jean-Michel Peyrin,Anselme L PerrierPubMed 36310215
- Neurogastroenterology and motility 34:e143542022Comparison of commercially available antibodies for the detection of phosphorylated alpha-synuclein in primary culture of ENS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimon Lassozé,Adrien de Guilhem de Lataillade,Thibauld Oullier,Michel Neunslist,Laurène Leclair-Visonneau,Pascal Derkinderen,Sébastien PaillussonPubMed 35279896
- Experimental neurobiology 31:29-412022Conformation-specific Antibodies Targeting Aggregated Forms of α-synuclein Block the Propagation of Synucleinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinsun Choi,Tae-Kyung Kim,Jinhyung Ahn,Jun Sung Lee,Byung Chul Jung,Sungwon An,Dongin Kim,Min Jae Lee,Inhee Mook-Jung,Sang Hoon Lee,Seung-Jae LeePubMed 35256542
- Disease models & mechanisms 15:2022A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhao-Feng Li,Lei Cui,Mi-Mi Jin,Dong-Yan Hu,Xiao-Gang Hou,Shu-Shu Liu,Xiong Zhang,Jian-Hong ZhuPubMed 35023548
- Acta neuropathologica communications 9:1892021Assembly of α-synuclein and neurodegeneration in the central nervous system of heterozygous M83 mice following the peripheral administration of α-synuclein seeds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer A Macdonald,John L Chen,Masami Masuda-Suzukake,Manuel Schweighauser,Zane Jaunmuktane,Thomas Warner,Janice L Holton,Annabelle Grossman,Richard Berks,Isabelle Lavenir,Michel GoedertPubMed 34819144
- NPJ Parkinson's disease 7:522021Potent inhibitors of toxic alpha-synuclein identified via cellular time-resolved FRET biosensors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnthony R Braun,Elly E Liao,Mian Horvath,Prakriti Kalra,Karen Acosta,Malaney C Young,Noah Nathan Kochen,Chih Hung Lo,Roland Brown,Michael D Evans,William C K Pomerantz,Elizabeth Rhoades,Kelvin Luk,Razvan L Cornea,David D Thomas,Jonathan N SachsPubMed 34183676
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SNCA phospho S129. The exact immunogen used to generate this antibody is proprietary information. Database link P37840
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/1000.00000 | Notes Please see WB protocol details in the image legend. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes Antigen retrieval: Microwave method - put the slice into 10 mmol/L citrate buffer (pH 6.0), microwave high temperature for 5 minutes, and then medium temperature for 15 minutes. Primary antibody incubation: 1 hour at 37°C Secondary antibody: Poly-HRP-Anti Mouse/Rabbit IgG, 50 μL for 30 minutes. DAB reagent. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor
Recommended products
Anti-Alpha-synuclein (phospho S129) antibody (ab59264) is a rabbit polyclonal antibody that is used to detect Alpha-synuclein in Western Blot, IHC-P. Suitable for Human, Mouse samples.
- Over 60 publications
- Trusted since 2007
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SNCA phospho S129. The exact immunogen used to generate this antibody is proprietary information. Database link P37840
- Purification technique
- Affinity purification Immunogen
- Specificity
Detects endogenous levels of Synuclein only when phosphorylated at serine 129. Due to 69% sequence homology ab59264 might react with Beta synuclein.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Anti-Alpha-synuclein (phospho S129) antibody (ab59264) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.
Trusted by the scientific community
Anti-Alpha-synuclein (phospho S129) (ab59264) was first used in a scientific publication in 2007 and has been cited over 60 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
- Biological function summary
The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.
- Pathways
Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.
- Associated diseases and disorders
Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-Alpha-synuclein (phospho S129) antibody (ab59264)
Blocking buffer: 5% (w/v) BSA in TBST.
Primary antibody dilution buffer: 5%（w/v）BSA，0.1%（v/v）, Tween-20 in TBST.
Secondary antibody dilution buffer: 5%（w/v）BSA，0.1%（v/v），Tween-20 in TBST.
12% SDS gel. Nitrocellulose membrane.
Blocking: Room temperature for 2 hours or overnight at 4°C. Then wash 3x for 5 minutes with 0.05% blocking buffer.
Primary antibody incubation: diluted in TBST at 1/500. Incubate overnight with 4 degrees shaking. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
Secondary antibody incubation: diluted in TBST at 1/2000. Incubate 37°C for 1 hour. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
ECL development.
All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody (ab59264) at 1/500 dilution
Lane 1: Mouse brain whole cell lysates at 40 µg
Lane 2: Mouse brain whole cell lysates at 40 µg with immunogen phosphopeptide
Predicted band size: 14 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody (ab59264)
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab59264 at a dilution of 1/50-1/100. Left hand image - without immunising peptide; right hand image - with immunising peptide.
Image courtesy of Sébastien Brot by customer review.Western blot - Anti-Alpha-synuclein (phospho S129) antibody (ab59264)
All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody (ab59264) at 1/1000 dilution
Lanes 1 - 3: Whole tissue lysate prepared from young transgenic mouse overexpressing human alpha-synuclein at 50 µg
Lanes 4 - 6: Whole tissue lysate prepared from old transgenic mouse overexpressing human alpha-synuclein at 50 µg
Lane 7: Whole tissue lysate prepared from KO mouse at 50 µg
SecondaryAll lanes: Goat anti-rabbit Ig (H+L) HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 14 kDa
Observed band size: 18 kDa, 19 kDa, 60 kDa, 80 kDa
Exposure time: 5min
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Alpha-synuclein (phospho S129) antibody (ab59264)
Alpha-synuclein (phospho S129) western blot using anti-Alpha-synuclein (phospho S129) antibody ab59264. Publication image and figure legend from Tang, L., Chen, C., et al., 2020, Evid Based Complement Alternat Med, PubMed 32655672.
ab59264 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab59264 please see the product overview.
Immunofluorescence staining of TH and α-syn protein in the substantia nigra of mice. Saline, blank group; Model, rotenone model group; Wsyg-H, high-dosage group; Wsyg-M, medium-dosage group; Wsyg-L, low-dosage group; Sinemet, positive control. ∗∗∗P < 0.001 vs. Saline; ##P < 0.01 and ###P < 0.001 vs. Model.
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