Anti-Alpha-synuclein (phospho S129) antibody

• WB

Supplier Data

Western blot - Anti-Alpha-synuclein (phospho S129) antibody (AB59264)

Blocking buffer : 5% (w/v) BSA in TBST.

Primary antibody dilution buffer : 5%（w/v）BSA，0.1%（v/v）, Tween-20 in TBST.

Secondary antibody dilution buffer : 5%（w/v）BSA，0.1%（v/v），Tween-20 in TBST.

12% SDS gel. Nitrocellulose membrane.

Blocking : Room temperature for 2 hours or overnight at 4°C. Then wash 3x for 5 minutes with 0.05% blocking buffer.

Primary antibody incubation : diluted in TBST at 1/500. Incubate overnight with 4 degrees shaking. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.

Secondary antibody incubation : diluted in TBST at 1/2000. Incubate 37°C for 1 hour. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.

ECL development.

All lanes:

Western blot - Anti-Alpha-synuclein (phospho S129) antibody (ab59264) at 1/500 dilution

Lane 1:

Mouse brain whole cell lysates at 40 µg

Lane 2:

Mouse brain whole cell lysates at 40 µg with immunogen phosphopeptide

Predicted band size: 14 kDa

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• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody (AB59264)

Immunohistochemical analysis of paraffin-embedded human brain tissue using ab59264 at a dilution of 1/50-1/100. Left hand image - without immunising peptide; right hand image - with immunising peptide.

• WB

AbReview24873****

Western blot - Anti-Alpha-synuclein (phospho S129) antibody (AB59264)

All lanes:

Western blot - Anti-Alpha-synuclein (phospho S129) antibody (ab59264) at 1/1000 dilution

Lanes 1 - 3:

Whole tissue lysate prepared from young transgenic mouse overexpressing human alpha-synuclein at 50 µg

Lanes 4 - 6:

Whole tissue lysate prepared from old transgenic mouse overexpressing human alpha-synuclein at 50 µg

Lane 7:

Whole tissue lysate prepared from KO mouse at 50 µg

Secondary

All lanes:

Goat anti-rabbit Ig (H+L) HRP at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 18 kDa,19 kDa,60 kDa,80 kDa

true

Exposure time: 5min

Image courtesy of Sébastien Brot by Abreview.

• WB

CiteAb

Western blot - Anti-Alpha-synuclein (phospho S129) antibody (AB59264)

Alpha-synuclein (phospho S129) western blot using anti-Alpha-synuclein (phospho S129) antibody ab59264. Publication image and figure legend from Tang, L., Chen, C., et al., 2020, Evid Based Complement Alternat Med, PubMed 32655672.

ab59264 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab59264 please see the product overview.

Immunofluorescence staining of TH and α-syn protein in the substantia nigra of mice. Saline, blank group; Model, rotenone model group; Wsyg-H, high-dosage group; Wsyg-M, medium-dosage group; Wsyg-L, low-dosage group; Sinemet, positive control. ***P < 0.001 vs. Saline; ##P < 0.01 and ###P < 0.001 vs. Model.

false