AB51253

Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]

Name: Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) | Abcam
Brand: Abcam Limited
SKU: AB51253
Price: 265 USD
Availability: InStock
Rating: 5 (9 reviews)

20ul selling size
BOND RX™ Validated
RabMAb
Recombinant
What is this?

(9 Reviews)

(541 Publications)

Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) is a rabbit monoclonal antibody detecting Alpha-synuclein in Western Blot, IHC-P, IHC-FrFl, ELISA, Dot Blot. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 370 publications
- Trusted since 2007

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

13 Images

Lab

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

All lanes:

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution

Lane 1:

Rat brain lysates, the membrane was incubated with alkaline phosphatase at 15 µg

Lane 2:

Rat brain lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

Predicted band size: 14 kDa

Observed band size: 18 kDa

false

Exposure time: 20s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

IHC image of alpha Synuclein (phospho S129) staining Human Parkinson Substantia Nigra tissue section*, previously antigen was retrieved by heat mediated with citrate buffer pH 6, fixed in formalin and embedded in paraffin. This section was incubated with ab51253 at 10 μg/mL for 15 mins at room temperature and detected using an HRP conjugated compact polymer system, performed on a Leica Bond™ system using the standard protocol F. DAB was used as the chromogen. Counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay performed on Human normal Substantia Nigra.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Immunohistochemistry image of Alpha Synuclein Phospho S129 staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex* performed on a Leica Biosystems BOND^® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab51253, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Immunohistochemistry - Free Floating - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

IHC-FrFl

PubMed

Immunohistochemistry - Free Floating - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Expression of WT aSyn in the SN is toxic over time. Mouse brain sections immunostained for TH (red panels) and aSyn (using ab51253) (green panels) 1 2 and 3 weeks after injection with vectors encoding for EGFP or WT aSyn. Scale bar for isolated channels 200 μm and for merged channels 100 μm.

This image is taken from Machado de Oliveira. R et al. PLoS Biol. 2017 Mar; 15(3): e2000374. S7a doi: 10.1371/journal.pbio.2000374 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

IHC-FrFl

Collaborator

Immunohistochemistry - Free Floating - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

IHC image of alpha Synuclein (phospho S129) staining in free floating tgM83^+/- or tgM83^+/+ mouse brain tissue. The section was incubated with ab51253 at 1/5000 dilution for 15 hours at 4^oC and detected using a Biotinylated conjugated Anti-Rabbit monocloal antibody at 1/200 dilution.

This image is courtesy of an abreview by Sophie Morgan

Unknown

Dot Blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Dot blot analysis of human alpha Synuclein (pS129) peptide (Lane 1), human alpha Synuclein (unmodified) peptide (Lane 2) labelling alpha Synuclein (pS129) with ab51253 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Supplier Data

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a higher sensitivity ECL substrate.

Band around 100kda corresponds to αS oligomer (PMID : 27637918, PMID : 12597857)

Lysates used here were prepared using RIPA method. We recommend 1% SDS hot lysate method to reduce the detection of oligomers. Please refer to Support & downloads -> Downloads -> Protocol booklet.

All lanes:

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution

Lane 1:

Mouse brain with Alzheimer's disease tissue lysate at 20 µg

Lane 2:

Mouse brain with Alzheimer's disease tissue lysate, membrane was incubated with alkaline phosphatase

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 14 kDa

Observed band size: 18 kDa,100 kDa

false

Lab

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 40 seconds

All lanes:

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution

Lane 1:

Mouse brain lysates, the membrane was incubated with alkaline phosphatase at 15 µg

Lane 2:

Mouse brain lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

Predicted band size: 14 kDa

Observed band size: 18 kDa

false

PubMed

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

α‐Synuclein (α‐syn) and S129‐phosphorylated α‐synuclein protein levels in SNCA/SNCA mouse brains after 12 days of treatment with 4mM ambroxol (Amb). (A) Western blotting for α‐synuclein (using ab1903) and serine 129 (S129)‐phosphorylated α‐synuclein protein (using ab51253) in the brainstem (example blots shown).

All lanes:

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

Predicted band size: 14 kDa

false

This image is taken from Migdalska-Richards A. et al. Ann Neurol. 2016 Nov; 80(5): 766–775. Fig 5 doi: 10.1002/ana.24790

Lab

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate. We suggest optimizing experimental protocols (increasing lysate amount, using lower antibody dilution or higher sensitivity ECL substrate) to improve results.

All lanes:

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa

true

Exposure time: 180s

ELISA - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

ELISA

Unknown

ELISA - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Direct ELISA antibody dose-response curve using ab51253.

Antibody concentration of 0-5000 ng/mL.

Antigen (Alpha-synuclein (pS129) phospho peptide and Alpha-synuclein non-phospho peptide) concentration of 1000 ng/mL.

An alkaline phosphatase conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

Collaborator

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

All lanes:

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution

Lane 1:

Sarkosyl-insoluble brain extract from mice transgenic for PrPA53T alpha-synuclein (line M83)

Lane 2:

Recombinant alpha-synuclein phosphorylated at S129, 3ng

Predicted band size: 14 kDa

false

This image is courtesy of Professor Michael Goedert’s team (Sophie Morgan and Masami Masuda-Suzukake).

Supplier Data

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Blocking and Diluting buffer and concentration - 5% NFDM/TBST

Exposure time : 3 seconds

All lanes:

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution

Lane 1:

In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of PLK2 (Polo-like kinase 2) but absence of AT

Lane 2:

In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of ATP but absence of PLK2 (Polo-like kinase 2

Lane 3:

In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of PLK2 (Polo-like kinase 2) and ATP

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 14 kDa

Observed band size: 17 kDa

false

Carrier free

Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1536Y

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

Dot, IHC-FrFl, ELISA, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects alpha synuclein phosphorylated on Ser129. IHC-P: This antibody showed no staining in human hippocampus normal brain and showed staining in Parkinson's brain as expected.

Mouse and rat species are recommended based on WB results, we do not guarantee IHC-P for mouse and rat.

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Reactivity data

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"IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "Good results have been obtained by treating the membrane with 0.4% PFA for 30 min at room temperature before blocking it with 5% milk.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFrFl-species-checked": "predicted", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFrFl-species-checked": "predicted", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFrFl-species-checked": "predicted", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Macaque monkey": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFrFl-species-checked": "predicted", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFrFl-species-checked": "predicted", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Pig": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFrFl-species-checked": "predicted", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Spider monkey": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFrFl-species-checked": "predicted", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Dot blot, ELISA, IHC-FrFl, IHC-P, WB in human, mouse, rat samples.

Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) has been cited over 373 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-Alpha-synuclein (phospho S129) antibody (Anti-pS129 alpha synuclin antibody/ Anti-SNCA antibody) [EP1536Y] (ab51253) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) has 9 independent reviews from customers.

Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) specifically detects Alpha-synuclein Phospho-S129 (UniProt ID: P37840; Molecular weight: 14kDa) and is sold in 100 µL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EP1536Y - ab209422.

Antibody clone EP1536Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 750, PE, Alexa Fluor® 647, Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 568 (ab321645, ab314419, ab225294, ab225295, ab311788, ab313069).

Alpha-synuclein phosphorylated at serine 129 (pS129; phosphorylated alpha synuclein, SNCA/NACP) is a modified form of the alpha-synuclein protein, which plays a crucial role in the regulation of synaptic vesicle trafficking and neurotransmitter release. This phosphorylation is significantly increased in the brains of patients with Parkinson's disease and other synucleinopathies. The presence of pS129 is associated with the formation of Lewy bodies, a hallmark of Parkinson's disease, and is believed to contribute to the neurotoxic properties of alpha-synuclein, exacerbating disease progression.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein also known as SNCA or PARK1 protein is a monomer protein with a molecular weight of approximately 14 kDa. It plays an important role in the synaptic functions of neurons. You usually find this protein expressed in the brain specifically in the presynaptic terminals of neurons. It also appears in other tissues including the heart and muscles though to a lesser degree. In its natural state alpha-synuclein exists as a soluble monomer but under certain conditions it can form insoluble protein filaments. The antibodies 4d6 and ep1536y might be used to study this protein providing tools to investigate its role and behavior in biological processes.

Biological function summary

Alpha-synuclein contributes to the regulation of synaptic vesicle trafficking and release. It interacts with the cell membrane and is involved in the assembly of SNARE complexes important for neurotransmitter release. Although alpha-synuclein generally acts in its monomeric form it may also aggregate and become involved in the assembly of fibrils under pathological conditions impacting normal cellular function. The protein's ability to interact with lipid membranes suggests its possible role in maintaining membrane integrity and dynamics.

Pathways

Alpha-synuclein is integral to neurotransmitter release and synaptic plasticity influencing dopaminergic signaling pathways. This protein interacts with other synaptic proteins like synaptophysin and synaptotagmin linking it to vesicular transport and recycling pathways. It plays a role in regulating dopamine biosynthesis and storage impacting neurological activity. The balance and regulation of these pathways are important for proper neural communication and function.

Alpha-synuclein is strongly associated with neurodegenerative diseases like Parkinson's disease and Lewy body dementia. The accumulation and aggregation of this protein in these disorders form Lewy bodies which are pathological hallmarks. Interactions with proteins like LRRK2 and Parkin also contribute to pathogenesis affecting cellular pathways involved in protein degradation and mitochondrial function. These interactions and aggregations implicate alpha-synuclein as a central figure in the progression and development of these neurological conditions.

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Target data

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed : 20798282, PubMed : 26442590, PubMed : 28288128, PubMed : 30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed : 28288128, PubMed : 30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed : 30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed : 20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed : 26442590).

See full target information SNCA phospho S129

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Publications (541)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8484 PubMed41006254

2025

Synaptic vesicle endocytosis deficits underlie cognitive dysfunction in mouse models of GBA-linked Parkinson's disease and dementia with Lewy bodies.

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D J Vidyadhara,David Bäckström,Risha Chakraborty,Jiapeng Ruan,Jae-Min Park,Pramod K Mistry,Sreeganga S Chandra

Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e70730 PubMed40990068

2025

PINK1 deficiency permits the development of Lewy body dementia with coexisting Aβ pathology.

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Tong-Yao Gao,Xu-Zheng Wang,Yu-Han Xie,Tong Wang,Yun-Bi Lu,Lu-Long Huang,Cong Chen,Ming Zhang,Xin Ma,Ya-Ling Chen,Fu-Xiang Liang,Zhi-Ling Lou,Jin-Sheng Li,Yi-Fan Yu,Jian-Bin Wu,Xiao-Ru Ma,Hua-Li Wang,Chun Tang,Wei-Ping Zhang

NPJ Parkinson's disease 11:254 PubMed40847026

2025

A brain-shuttled antibody targeting alpha synuclein aggregates for the treatment of synucleinopathies.

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Sungwon An,John J McInnis,Dongin Kim,Yihang Li,Ozge Tasdemir-Yilmaz,Jinhyung Ahn,Brian C Mackness,Seung-Hwan Kwon,Julia Maeve Bonner,Miran Yoo,Simon Dujardin,Donghwan Kim,Jinyoung Park,Hyesu Yun,Yi Tang,Laurent Pradier,Sumin Hyeon,Daehae Song,Byungje Sung,Rajaraman Krishnan,Brian Spencer,Robert A Rissman,Jagdeep K Sandhu,Arsalan S Haqqani,Jung-Won Shin,Donghwan B Kim,Hyeran Lee,Jinwon Jung,Weon-Kyoo You,Alexandra T Star,Christie E Delaney,Danica B Stanimirovic,Sergio Pablo Sardi,Sang Hoon Lee,Can Kayatekin

Nature communications 16:7797 PubMed40841722

2025

Integrating artificial intelligence and optogenetics for Parkinson's disease diagnosis and therapeutics in male mice.

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Bobae Hyeon,Jaehyun Shin,Jae-Hun Lee,Woori Kim,Jea Kwon,Heeyoung Lee,Dae-Gun Kim,Choong Yeon Kim,Sian Choi,Jae-Woong Jeong,Kwang-Soo Kim,C Justin Lee,Daesoo Kim,Won Do Heo

Frontiers in cellular neuroscience 19:1557645 PubMed40842561

2025

Decreased neuronal and increased endothelial fractalkine expression are associated with neuroinflammation in Parkinson's disease and related disorders.

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Yaping Chu,Ashley S Harms,Ashley Boehringer,Jeffrey H Kordower

Drug delivery and translational research : PubMed40836186

2025

Polymeric nanoparticle-mediated GBA1 gene therapy is neuroprotective in a preclinical model of Parkinson's disease.

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Mohit Kwatra,Gijung Kwak,Haolin Li,Jung Soo Suk,Han Seok Ko

NPJ Parkinson's disease 11:242 PubMed40804075

2025

Early synaptic changes and reduced brain connectivity in PD-like mice with depressive phenotype.

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Lluis Miquel-Rio,Judith Jericó-Escolar,Unai Sarriés-Serrano,Claudia Yanes-Castilla,María Torres-López,Uxia Argibay,Verónica Paz,Carme Casal,Emma Muñoz-Moreno,Xavier López-Gil,Analia Bortolozzi

Nature communications 16:7368 PubMed40783395

2025

Design of Ig-like binders targeting α-synuclein fibril for mitigating its pathological activities.

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Shuyi Zeng,Xingyu Xiong,Houfang Long,Qianhui Xu,Yifan Yu,Bo Sun,Cong Liu,Zhizhi Wang,Wenqing Xu,Shengnan Zhang,Dan Li

PloS one 20:e0329823 PubMed40779487

2025

Expression of human A53T alpha-synuclein without endogenous rat alpha-synuclein fails to elicit Parkinson's disease-related phenotypes in a novel humanized rat model.

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Nicole K Polinski,Jukka Puoliväli,Leena Rauhala,Taina-Kaisa Stenius,Timo Bragge,Teija Parkkari,Anna-Maija Penttinen,Yi Chen,Omar S Mabrouk,Kelly E Glajch,Warren D Hirst,Michael Perkinton,Terina N Martinez,Mark A Frasier

NPJ Parkinson's disease 11:232 PubMed40775228

2025

Phosphorylated α-synuclein in CSF and plasma does not reflect synucleinopathy.

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Giovanni Bellomo,Erik Stoops,Jeroen Vanbrabant,Leentje Demeyer,Cindy Francois,Melanie Vanhooren,Yihua Ma,Carly M Farris,Luis Concha-Marambio,Federico Paolini Paoletti,Lorenzo Gaetani,Lucilla Parnetti,Davide Chiasserini

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Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Immunohistochemistry - Free Floating - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Immunohistochemistry - Free Floating - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Dot Blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

ELISA - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253)

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Publications (541)

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