Rabbit anti-Alpha-synuclein antibody EP1536Y ab51253 is a rabbit monoclonal antibody that is used in Alpha-synuclein western blotting, IHC and flow cytometry. Suitable for human, mouse and rat samples.
Recombinant format for high batch-to-batch consistency and reproducible results
Validated for Alpha-synuclein staining on the Leica BOND™ RX automated IHC staining platform
Tried and trusted by researchers since 2007
Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-P | IHC-FrFl | IP | Flow Cyt | ELISA | Dot | WB | IHC-Fr | |
---|---|---|---|---|---|---|---|---|
Human | Tested | Expected | Not recommended | Not recommended | Tested | Tested | Expected | Not recommended |
Mouse | Not recommended | Tested | Not recommended | Not recommended | Expected | Expected | Tested | Not recommended |
Rat | Not recommended | Expected | Not recommended | Not recommended | Expected | Expected | Tested | Not recommended |
Chimpanzee | Not recommended | Predicted | Not recommended | Not recommended | Predicted | Predicted | Predicted | Not recommended |
Cow | Not recommended | Predicted | Not recommended | Not recommended | Predicted | Predicted | Predicted | Not recommended |
Gorilla | Not recommended | Predicted | Not recommended | Not recommended | Predicted | Predicted | Predicted | Not recommended |
Macaque monkey | Not recommended | Predicted | Not recommended | Not recommended | Predicted | Predicted | Predicted | Not recommended |
Orangutan | Not recommended | Predicted | Not recommended | Not recommended | Predicted | Predicted | Predicted | Not recommended |
Pig | Not recommended | Predicted | Not recommended | Not recommended | Predicted | Predicted | Predicted | Not recommended |
Spider monkey | Not recommended | Predicted | Not recommended | Not recommended | Predicted | Predicted | Predicted | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5-10 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Cow | Dilution info - | Notes - |
Species Pig | Dilution info - | Notes - |
Species Chimpanzee | Dilution info - | Notes - |
Species Macaque monkey | Dilution info - | Notes - |
Species Gorilla | Dilution info - | Notes - |
Species Orangutan | Dilution info - | Notes - |
Species Spider monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes Good results have been obtained by treating the membrane with 0.4% PFA for 30 min at room temperature before blocking it with 5% milk. |
Species Rat | Dilution info 1/1000 - 1/5000 | Notes Good results have been obtained by treating the membrane with 0.4% PFA for 30 min at room temperature before blocking it with 5% milk. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey | Dilution info - | Notes - |
Select an associated product type
Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).
Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP, SNCA, NACP, PARK1
Rabbit anti-Alpha-synuclein antibody EP1536Y ab51253 is a rabbit monoclonal antibody that is used in Alpha-synuclein western blotting, IHC and flow cytometry. Suitable for human, mouse and rat samples.
Recombinant format for high batch-to-batch consistency and reproducible results
Validated for Alpha-synuclein staining on the Leica BOND™ RX automated IHC staining platform
Tried and trusted by researchers since 2007
Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation.
Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP, SNCA, NACP, PARK1
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EP1536Y
Affinity purification Protein A
This antibody only detects alpha synuclein phosphorylated on Ser129. IHC-P: This antibody showed no staining in human hippocampus normal brain and showed staining in Parkinson's brain as expected.
Mouse and rat species are recommended based on WB results, we do not guarantee IHC-FrFl and IHC-P for Mouse and rat.
Blue Ice
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Alpha-synuclein also known as SNCA or PARK1 protein is a monomer protein with a molecular weight of approximately 14 kDa. It plays an important role in the synaptic functions of neurons. You usually find this protein expressed in the brain specifically in the presynaptic terminals of neurons. It also appears in other tissues including the heart and muscles though to a lesser degree. In its natural state alpha-synuclein exists as a soluble monomer but under certain conditions it can form insoluble protein filaments. The antibodies 4d6 and ep1536y might be used to study this protein providing tools to investigate its role and behavior in biological processes.
Alpha-synuclein contributes to the regulation of synaptic vesicle trafficking and release. It interacts with the cell membrane and is involved in the assembly of SNARE complexes important for neurotransmitter release. Although alpha-synuclein generally acts in its monomeric form it may also aggregate and become involved in the assembly of fibrils under pathological conditions impacting normal cellular function. The protein's ability to interact with lipid membranes suggests its possible role in maintaining membrane integrity and dynamics.
Alpha-synuclein is integral to neurotransmitter release and synaptic plasticity influencing dopaminergic signaling pathways. This protein interacts with other synaptic proteins like synaptophysin and synaptotagmin linking it to vesicular transport and recycling pathways. It plays a role in regulating dopamine biosynthesis and storage impacting neurological activity. The balance and regulation of these pathways are important for proper neural communication and function.
Alpha-synuclein is strongly associated with neurodegenerative diseases like Parkinson's disease and Lewy body dementia. The accumulation and aggregation of this protein in these disorders form Lewy bodies which are pathological hallmarks. Interactions with proteins like LRRK2 and Parkin also contribute to pathogenesis affecting cellular pathways involved in protein degradation and mitochondrial function. These interactions and aggregations implicate alpha-synuclein as a central figure in the progression and development of these neurological conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Phospho alpha-synuclein immunohistochemistry staining of mouse brain using rabbit anti-Phospho alpha-synuclein antibody
Expression of WT aSyn in the SN is toxic over time. Mouse brain sections immunostained for TH (red panels) and aSyn (using ab51253) (green panels) 1 2 and 3 weeks after injection with vectors encoding for EGFP or WT aSyn. Scale bar for isolated channels 200 μm and for merged channels 100 μm.
α‐Synuclein (α‐syn) and S129‐phosphorylated α‐synuclein protein levels in SNCA/SNCA mouse brains after 12 days of treatment with 4mM ambroxol (Amb). (A) Western blotting for α‐synuclein (using Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free ab1903) and serine 129 (S129)‐phosphorylated α‐synuclein protein (using ab51253) in the brainstem (example blots shown).
All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)
Predicted band size: 14 kDa
Blocking and Diluting buffer and concentration - 5% NFDM/TBST
Exposure time: 3 seconds
All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution
Lane 1: In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of PLK2 (Polo-like kinase 2) but absence of AT
Lane 2: In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of ATP but absence of PLK2 (Polo-like kinase 2
Lane 3: In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of PLK2 (Polo-like kinase 2) and ATP
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 20 seconds
All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution
Lane 1: Rat brain lysates, the membrane was incubated with alkaline phosphatase. at 15 µg
Lane 2: Rat brain lysates at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 18 kDa
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 40 seconds
All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution
Lane 1: Mouse brain lysates, the membrane was incubated with alkaline phosphatase at 15 µg
Lane 2: Mouse brain lysates at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 18 kDa
All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution
Lane 1: Sarkosyl-insoluble brain extract from mice transgenic for PrPA53T alpha-synuclein (line M83)
Lane 2: Recombinant alpha-synuclein phosphorylated at S129, 3ng
Predicted band size: 14 kDa
IHC image of alpha Synuclein (phospho S129) staining Human Parkinson Substantia Nigra tissue section*, previously antigen was retrieved by heat mediated with citrate buffer pH 6, fixed in formalin and embedded in paraffin. This section was incubated with ab51253 at 10 μg/mL for 15 mins at room temperature and detected using an HRP conjugated compact polymer system, performed on a Leica Bond™ system using the standard protocol F. DAB was used as the chromogen. Counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay performed on Human normal Substantia Nigra.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Dot blot analysis of human alpha Synuclein (pS129) peptide (Lane 1), huamn alpha Synuclein (unmodified) peptide (Lane 2) labelling alpha Synuclein (pS129) with ab51253 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Phospho alpha-synuclein immunohistochemistry staining of mouse brain using rabbit anti-Phospho alpha-synuclein antibody
IHC image of alpha Synuclein (phospho S129) staining in free floating tgM83+/- or tgM83+/+ mouse brain tissue. The section was incubated with ab51253 at 1/5000 dilution for 15 hours at 4oC and detected using a Biotinylated conjugated Anti-Rabbit monocloal antibody at 1/200 dilution.
Direct ELISA antibody dose-response curve using ab51253.
Antibody concentration of 0-5000 ng/mL.
Antigen (Alpha-synuclein (pS129) phospho peptide and Alpha-synuclein non-phospho peptide) concentration of 1000 ng/mL.
An alkaline phosphatase conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a higher sensitivity ECL substrate.
Band around 100kda corresponds to αS oligomer (PMID: 27637918, PMID: 12597857)
Lysates used here were prepared using RIPA method. We recommend 1% SDS hot lysate method to reduce the detection of oligomers. Please refer to Support & downloads -> Downloads -> Protocol booklet.
All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution
Lane 1: Mouse brain with Alzheimer’s disease tissue lysate at 20 µg
Lane 2: Mouse brain with Alzheimer’s disease tissue lysate, membrane was incubated with alkaline phosphatase
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 18 kDa, 100 kDa
Immunohistochemistry image of Alpha Synuclein Phospho S129 staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex* performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab51253, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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