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Rabbit Recombinant Monoclonal Alpha-synuclein phospho S129 antibody. Suitable for IHC-P, IHC-FrFl, ELISA, Dot, WB and reacts with Human, Mouse, Rat samples. Cited in 374 publications.
Alternative names=Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP, SNCA, NACP, PARK1
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IHC-FrFl | IP | Flow Cyt | ELISA | Dot | WB | IHC-Fr | |
|---|---|---|---|---|---|---|---|---|
| Human | Tested | Expected | Not recommended | Not recommended | Tested | Tested | Expected | Not recommended |
| Mouse | Not recommended | Tested | Not recommended | Not recommended | Expected | Expected | Tested | Not recommended |
| Rat | Not recommended | Expected | Not recommended | Not recommended | Expected | Expected | Tested | Not recommended |
| Chimpanzee | Not recommended | Predicted | Not recommended | Not recommended | Predicted | Predicted | Predicted | Not recommended |
| Cow | Not recommended | Predicted | Not recommended | Not recommended | Predicted | Predicted | Predicted | Not recommended |
| Gorilla | Not recommended | Predicted | Not recommended | Not recommended | Predicted | Predicted | Predicted | Not recommended |
| Macaque monkey | Not recommended | Predicted | Not recommended | Not recommended | Predicted | Predicted | Predicted | Not recommended |
| Orangutan | Not recommended | Predicted | Not recommended | Not recommended | Predicted | Predicted | Predicted | Not recommended |
| Pig | Not recommended | Predicted | Not recommended | Not recommended | Predicted | Predicted | Predicted | Not recommended |
| Spider monkey | Not recommended | Predicted | Not recommended | Not recommended | Predicted | Predicted | Predicted | Not recommended |
Images
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-10 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Cow | Dilution info - | Notes - |
Species Pig | Dilution info - | Notes - |
Species Chimpanzee | Dilution info - | Notes - |
Species Macaque monkey | Dilution info - | Notes - |
Species Gorilla | Dilution info - | Notes - |
Species Orangutan | Dilution info - | Notes - |
Species Spider monkey | Dilution info - | Notes - |
IHC-FrFl
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes Good results have been obtained by treating the membrane with 0.4% PFA for 30 min at room temperature before blocking it with 5% milk. |
Species Rat | Dilution info 1/1000 - 1/5000 | Notes Good results have been obtained by treating the membrane with 0.4% PFA for 30 min at room temperature before blocking it with 5% milk. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey | Dilution info - | Notes - |
Recommended products
Rabbit Recombinant Monoclonal Alpha-synuclein phospho S129 antibody. Suitable for IHC-P, IHC-FrFl, ELISA, Dot, WB and reacts with Human, Mouse, Rat samples. Cited in 374 publications.
Alternative names=Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP, SNCA, NACP, PARK1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EP1536Y
- Purification technique
Affinity purification Protein A
- Specificity
This antibody only detects alpha synuclein phosphorylated on Ser129. IHC-P: This antibody showed no staining in human hippocampus normal brain and showed staining in Parkinson's brain as expected.
Mouse and rat species are recommended based on WB results, we do not guarantee IHC-FrFl and IHC-P for Mouse and rat.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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