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Rabbit Recombinant Monoclonal Alpha-synuclein phospho S129 antibody. Suitable for IHC-P, IHC-FrFl, ELISA, Dot, WB and reacts with Human, Mouse, Rat samples. Cited in 374 publications.

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIHC-FrFlIPFlow CytELISADotWBIHC-Fr
Human
Tested
Expected
Not recommended
Not recommended
Tested
Tested
Expected
Not recommended
Mouse
Not recommended
Tested
Not recommended
Not recommended
Expected
Expected
Tested
Not recommended
Rat
Not recommended
Expected
Not recommended
Not recommended
Expected
Expected
Tested
Not recommended
Chimpanzee
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Predicted
Predicted
Not recommended
Cow
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Predicted
Predicted
Not recommended
Gorilla
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Predicted
Predicted
Not recommended
Macaque monkey
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Predicted
Predicted
Not recommended
Orangutan
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Predicted
Predicted
Not recommended
Pig
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Predicted
Predicted
Not recommended
Spider monkey
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Predicted
Predicted
Not recommended

Images

Tested
Tested

Species
Human
Dilution info
5-10 µg/mL
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species
Cow
Dilution info
-
Notes

-

Species
Pig
Dilution info
-
Notes

-

Species
Chimpanzee
Dilution info
-
Notes

-

Species
Macaque monkey
Dilution info
-
Notes

-

Species
Gorilla
Dilution info
-
Notes

-

Species
Orangutan
Dilution info
-
Notes

-

Species
Spider monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/5000
Notes

Good results have been obtained by treating the membrane with 0.4% PFA for 30 min at room temperature before blocking it with 5% milk.

Species
Rat
Dilution info
1/1000 - 1/5000
Notes

Good results have been obtained by treating the membrane with 0.4% PFA for 30 min at room temperature before blocking it with 5% milk.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey
Dilution info
-
Notes

-

Recommended products

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Rabbit Recombinant Monoclonal Alpha-synuclein phospho S129 antibody. Suitable for IHC-P, IHC-FrFl, ELISA, Dot, WB and reacts with Human, Mouse, Rat samples. Cited in 374 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EP1536Y

Purification technique

Affinity purification Protein A

Specificity

This antibody only detects alpha synuclein phosphorylated on Ser129. IHC-P: This antibody showed no staining in human hippocampus normal brain and showed staining in Parkinson's brain as expected.

Mouse and rat species are recommended based on WB results, we do not guarantee IHC-FrFl and IHC-P for Mouse and rat.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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