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Rabbit anti-Alpha-synuclein antibody EP1536Y ab51253 is a rabbit monoclonal antibody that is used in Alpha-synuclein western blotting, IHC and flow cytometry. Suitable for human, mouse and rat samples.

Recombinant format for high batch-to-batch consistency and reproducible results
Validated for Alpha-synuclein staining on the Leica BOND™ RX automated IHC staining platform
Tried and trusted by researchers since 2007
Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation.


Images

Immunohistochemistry - Free Floating - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253), expandable thumbnail
  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253), expandable thumbnail
  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253), expandable thumbnail
  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253), expandable thumbnail
  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (AB51253), expandable thumbnail

Publications

  • Nature communications 14:83682023
    NEMO reshapes the α-Synuclein aggregate interface and acts as an autophagy adapter by co-condensation with p62.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Nikolas Furthmann,Verian Bader,Lena Angersbach,Alina Blusch,Simran Goel,Ana Sánchez-Vicente,Laura J Krause,Sarah A Chaban,Prerna Grover,Victoria A Trinkaus,Eva M van Well,Maximilian Jaugstetter,Kristina Tschulik,Rune Busk Damgaard,Carsten Saft,Gisa Ellrichmann,Ralf Gold,Arend Koch,Benjamin Englert,Ana Westenberger,Christine Klein,Lisa Jungbluth,Carsten Sachse,Christian Behrends,Markus Glatzel,F Ulrich Hartl,Ken Nakamura,Chadwick W Christine,Eric J Huang,Jörg Tatzelt,Konstanze F Winklhofer
    PubMed 38114471
  • Biomolecules 13:2023
    Protease-Sensitive and -Resistant Forms of Human and Murine Alpha-Synucleins in Distinct Brain Regions of Transgenic Mice (M83) Expressing the Human Mutated A53T Protein.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Dominique Bétemps,Jean-Noël Arsac,Simon Nicot,Dominique Canal,Habiba Tlili,Maxime Belondrade,Eric Morignat,Jérémy Verchère,Damien Gaillard,Lilian Bruyère-Ostells,Charly Mayran,Latifa Lakhdar,Daisy Bougard,Thierry Baron
    PubMed 38136658

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIHC-FrFlIPFlow CytELISADotWBIHC-Fr
Human
Tested
Expected
Not recommended
Not recommended
Tested
Tested
Expected
Not recommended
Mouse
Not recommended
Tested
Not recommended
Not recommended
Expected
Expected
Tested
Not recommended
Rat
Not recommended
Expected
Not recommended
Not recommended
Expected
Expected
Tested
Not recommended
Chimpanzee
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Predicted
Predicted
Not recommended
Cow
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Predicted
Predicted
Not recommended
Gorilla
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Predicted
Predicted
Not recommended
Macaque monkey
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Predicted
Predicted
Not recommended
Orangutan
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Predicted
Predicted
Not recommended
Pig
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Predicted
Predicted
Not recommended
Spider monkey
Not recommended
Predicted
Not recommended
Not recommended
Predicted
Predicted
Predicted
Not recommended

Tested
Tested

Species

Human

Dilution info

5-10 µg/mL

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Cow

Dilution info

-

Notes

-

Species

Pig

Dilution info

-

Notes

-

Species

Chimpanzee

Dilution info

-

Notes

-

Species

Macaque monkey

Dilution info

-

Notes

-

Species

Gorilla

Dilution info

-

Notes

-

Species

Orangutan

Dilution info

-

Notes

-

Species

Spider monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/5000

Notes

Good results have been obtained by treating the membrane with 0.4% PFA for 30 min at room temperature before blocking it with 5% milk.

Species

Rat

Dilution info

1/1000 - 1/5000

Notes

Good results have been obtained by treating the membrane with 0.4% PFA for 30 min at room temperature before blocking it with 5% milk.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan, Spider monkey

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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2 products for Alternative Version

Target data

Function

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).

Alternative names

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Rabbit anti-Alpha-synuclein antibody EP1536Y ab51253 is a rabbit monoclonal antibody that is used in Alpha-synuclein western blotting, IHC and flow cytometry. Suitable for human, mouse and rat samples.

Recombinant format for high batch-to-batch consistency and reproducible results
Validated for Alpha-synuclein staining on the Leica BOND™ RX automated IHC staining platform
Tried and trusted by researchers since 2007
Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP1536Y

Purification technique

Affinity purification Protein A

Specificity

This antibody only detects alpha synuclein phosphorylated on Ser129. IHC-P: This antibody showed no staining in human hippocampus normal brain and showed staining in Parkinson's brain as expected.

Mouse and rat species are recommended based on WB results, we do not guarantee IHC-FrFl and IHC-P for Mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Activity summary

Alpha-synuclein also known as SNCA or PARK1 protein is a monomer protein with a molecular weight of approximately 14 kDa. It plays an important role in the synaptic functions of neurons. You usually find this protein expressed in the brain specifically in the presynaptic terminals of neurons. It also appears in other tissues including the heart and muscles though to a lesser degree. In its natural state alpha-synuclein exists as a soluble monomer but under certain conditions it can form insoluble protein filaments. The antibodies 4d6 and ep1536y might be used to study this protein providing tools to investigate its role and behavior in biological processes.

Biological function summary

Alpha-synuclein contributes to the regulation of synaptic vesicle trafficking and release. It interacts with the cell membrane and is involved in the assembly of SNARE complexes important for neurotransmitter release. Although alpha-synuclein generally acts in its monomeric form it may also aggregate and become involved in the assembly of fibrils under pathological conditions impacting normal cellular function. The protein's ability to interact with lipid membranes suggests its possible role in maintaining membrane integrity and dynamics.

Pathways

Alpha-synuclein is integral to neurotransmitter release and synaptic plasticity influencing dopaminergic signaling pathways. This protein interacts with other synaptic proteins like synaptophysin and synaptotagmin linking it to vesicular transport and recycling pathways. It plays a role in regulating dopamine biosynthesis and storage impacting neurological activity. The balance and regulation of these pathways are important for proper neural communication and function.

Associated diseases and disorders

Alpha-synuclein is strongly associated with neurodegenerative diseases like Parkinson's disease and Lewy body dementia. The accumulation and aggregation of this protein in these disorders form Lewy bodies which are pathological hallmarks. Interactions with proteins like LRRK2 and Parkin also contribute to pathogenesis affecting cellular pathways involved in protein degradation and mitochondrial function. These interactions and aggregations implicate alpha-synuclein as a central figure in the progression and development of these neurological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Immunohistochemistry - Free Floating - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253), expandable thumbnail
    This image is taken from Machado de Oliveira. R et al. PLoS Biol. 2017 Mar; 15(3): e2000374. S7a doi: 10.1371/journal.pbio.2000374 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

    Immunohistochemistry - Free Floating - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

    Phospho alpha-synuclein immunohistochemistry staining of mouse brain using rabbit anti-Phospho alpha-synuclein antibody

    Expression of WT aSyn in the SN is toxic over time. Mouse brain sections immunostained for TH (red panels) and aSyn (using ab51253) (green panels) 1 2 and 3 weeks after injection with vectors encoding for EGFP or WT aSyn. Scale bar for isolated channels 200 μm and for merged channels 100 μm.

  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253), expandable thumbnail
    This image is taken from Migdalska-Richards A. et al. Ann Neurol. 2016 Nov; 80(5): 766–775. Fig 5 doi: 10.1002/ana.24790

    Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

    α‐Synuclein (α‐syn) and S129‐phosphorylated α‐synuclein protein levels in SNCA/SNCA mouse brains after 12 days of treatment with 4mM ambroxol (Amb). (A) Western blotting for α‐synuclein (using Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free ab1903) and serine 129 (S129)‐phosphorylated α‐synuclein protein (using ab51253) in the brainstem (example blots shown).

    All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

    Predicted band size: 14 kDa

  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253), expandable thumbnail

    Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

    Blocking and Diluting buffer and concentration - 5% NFDM/TBST

    Exposure time: 3 seconds

    All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution

    Lane 1: In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of PLK2 (Polo-like kinase 2) but absence of AT

    Lane 2: In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of ATP but absence of PLK2 (Polo-like kinase 2

    Lane 3: In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of PLK2 (Polo-like kinase 2) and ATP

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa

    Observed band size: 17 kDa

  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253), expandable thumbnail

    Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 20 seconds

    All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution

    Lane 1: Rat brain lysates, the membrane was incubated with alkaline phosphatase. at 15 µg

    Lane 2: Rat brain lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution

    Predicted band size: 14 kDa

    Observed band size: 18 kDa

  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253), expandable thumbnail

    Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 40 seconds

    All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution

    Lane 1: Mouse brain lysates, the membrane was incubated with alkaline phosphatase at 15 µg

    Lane 2: Mouse brain lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution

    Predicted band size: 14 kDa

    Observed band size: 18 kDa

  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253), expandable thumbnail
    This image is courtesy of Professor Michael Goedert’s team (Sophie Morgan and Masami Masuda-Suzukake).

    Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

    All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution

    Lane 1: Sarkosyl-insoluble brain extract from mice transgenic for PrPA53T alpha-synuclein (line M83)

    Lane 2: Recombinant alpha-synuclein phosphorylated at S129, 3ng

    Predicted band size: 14 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

    IHC image of alpha Synuclein (phospho S129) staining Human Parkinson Substantia Nigra tissue section*, previously antigen was retrieved by heat mediated with citrate buffer pH 6, fixed in formalin and embedded in paraffin. This section was incubated with ab51253 at 10 μg/mL for 15 mins at room temperature and detected using an HRP conjugated compact polymer system, performed on a Leica Bond™ system using the standard protocol F. DAB was used as the chromogen. Counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay performed on Human normal Substantia Nigra.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Dot Blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253), expandable thumbnail

    Dot Blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

    Dot blot analysis of human alpha Synuclein (pS129) peptide (Lane 1), huamn alpha Synuclein (unmodified) peptide (Lane 2) labelling alpha Synuclein (pS129) with ab51253 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry - Free Floating - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253), expandable thumbnail
    This image is courtesy of a customer review by Sophie Morgan

    Immunohistochemistry - Free Floating - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

    Phospho alpha-synuclein immunohistochemistry staining of mouse brain using rabbit anti-Phospho alpha-synuclein antibody

    IHC image of alpha Synuclein (phospho S129) staining in free floating tgM83+/- or tgM83+/+ mouse brain tissue. The section was incubated with ab51253 at 1/5000 dilution for 15 hours at 4oC and detected using a Biotinylated conjugated Anti-Rabbit monocloal antibody at 1/200 dilution.

  • ELISA - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253), expandable thumbnail

    ELISA - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

    Direct ELISA antibody dose-response curve using ab51253.

    Antibody concentration of 0-5000 ng/mL.

    Antigen (Alpha-synuclein (pS129) phospho peptide and Alpha-synuclein non-phospho peptide) concentration of 1000 ng/mL.

    An alkaline phosphatase conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253), expandable thumbnail

    Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This blot was developed using a higher sensitivity ECL substrate.

    Band around 100kda corresponds to αS oligomer (PMID: 27637918, PMID: 12597857)

    Lysates used here were prepared using RIPA method. We recommend 1% SDS hot lysate method to reduce the detection of oligomers. Please refer to Support & downloads -> Downloads -> Protocol booklet.

    All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution

    Lane 1: Mouse brain with Alzheimer’s disease tissue lysate at 20 µg

    Lane 2: Mouse brain with Alzheimer’s disease tissue lysate, membrane was incubated with alkaline phosphatase

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa

    Observed band size: 18 kDa, 100 kDa

  • Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)

    Immunohistochemistry image of Alpha Synuclein Phospho S129 staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex* performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab51253, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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