Phospho Alpha-synuclein antibody pS129 [EP1536Y] ab209422 is a carrier free rabbit monoclonal antibody that is used in Alpha-synuclein western blotting, ELISA, IHC and Dot. Suitable for human, mouse and rat samples.
- PBS only, conjugation-ready, removing anything extra from your antibodies, greater flexibility in assay design
- Validated on the Leica BOND™ RX automated IHC staining platform for Alpha-synuclein IHC
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EP1536Y is cited in over 410 publications
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IHC-P | IHC-FrFl | ELISA | Dot | WB | IP | Flow Cyt | IHC-Fr | |
---|---|---|---|---|---|---|---|---|
Human | Tested | Expected | Tested | Tested | Expected | Not recommended | Not recommended | Not recommended |
Mouse | Not recommended | Tested | Expected | Expected | Tested | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Expected | Expected | Expected | Tested | Not recommended | Not recommended | Not recommended |
Chimpanzee | Not recommended | Predicted | Predicted | Predicted | Predicted | Not recommended | Not recommended | Not recommended |
Cow | Not recommended | Predicted | Predicted | Predicted | Predicted | Not recommended | Not recommended | Not recommended |
Gorilla | Not recommended | Predicted | Predicted | Predicted | Predicted | Not recommended | Not recommended | Not recommended |
Macaque monkey | Not recommended | Predicted | Predicted | Predicted | Predicted | Not recommended | Not recommended | Not recommended |
Orangutan | Not recommended | Predicted | Predicted | Predicted | Predicted | Not recommended | Not recommended | Not recommended |
Pig | Not recommended | Predicted | Predicted | Predicted | Predicted | Not recommended | Not recommended | Not recommended |
Spider monkey | Not recommended | Predicted | Predicted | Predicted | Predicted | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Orangutan | Dilution info - | Notes - |
Species Pig | Dilution info - | Notes - |
Species Chimpanzee | Dilution info - | Notes - |
Species Macaque monkey | Dilution info - | Notes - |
Species Gorilla | Dilution info - | Notes - |
Species Cow | Dilution info - | Notes - |
Species Spider monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Orangutan, Pig, Chimpanzee, Macaque monkey, Gorilla, Cow, Spider monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Orangutan, Pig, Chimpanzee, Macaque monkey, Gorilla, Cow, Spider monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Orangutan, Pig, Chimpanzee, Macaque monkey, Gorilla, Cow, Spider monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Orangutan, Pig, Chimpanzee, Macaque monkey, Gorilla, Cow, Spider monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Orangutan, Pig, Chimpanzee, Macaque monkey, Gorilla, Cow, Spider monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Orangutan, Pig, Chimpanzee, Macaque monkey, Gorilla, Cow, Spider monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Orangutan, Pig, Chimpanzee, Macaque monkey, Gorilla, Cow, Spider monkey | Dilution info - | Notes - |
Select an associated product type
Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:20798282, PubMed:26442590, PubMed:28288128, PubMed:30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).
NACP, PARK1, SNCA, NACP, PARK1, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP
Phospho Alpha-synuclein antibody pS129 [EP1536Y] ab209422 is a carrier free rabbit monoclonal antibody that is used in Alpha-synuclein western blotting, ELISA, IHC and Dot. Suitable for human, mouse and rat samples.
- PBS only, conjugation-ready, removing anything extra from your antibodies, greater flexibility in assay design
- Validated on the Leica BOND™ RX automated IHC staining platform for Alpha-synuclein IHC
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EP1536Y is cited in over 410 publications
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EP1536Y
Affinity purification Protein A
This antibody only detects alpha synuclein phosphorylated on Ser129. IHC-P: This antibody showed no staining in human hippocampus normal brain and showed staining in Parkinson's brain as expected.
Mouse and rat species are recommended based on WB results, we do not guarantee IHC-FrFl and IHC-P for Mouse and rat.
Blue Ice
+4°C
+4°C
Do Not Freeze
ab209422 is the carrier-free version of Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.
Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.
Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This IHC data was generated using the same anti-phospho alpha synuclein S129 antibody clone, EP1536Y, in a different buffer formulation (cat# Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253).
IHC image of alpha Synuclein (phospho S129) staining in Human Parkinson Substantia Nigra formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay performed on Human normal Substantia Nigra.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Dot blot analysis of alpha Synuclein (pS129) peptide (Lane 1), alpha Synuclein (unmodified) peptide (Lane 2) labelling alpha Synuclein (pS129) with Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253).
This ELISA data was generated using the same anti-phospho alpha synuclein S129 antibody clone, EP1536Y, in a different buffer formulation (cat# Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253).
Direct ELISA antibody dose-response curve using Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253. Antibody concentration of 0-5000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
This data was developed using Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253, the same antibody clone in a different buffer formulation.
Immunohistochemistry image of Alpha Synuclein Phospho S129 staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex* performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
This data was developed using Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253, the same antibody clone in a different buffer.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a higher sensitivity ECL substrate.
Band around 100kda corresponds to αS oligomer (PMID: 27637918, PMID: 12597857)
Lysates used here were prepared using RIPA method. We recommend 1% SDS hot lysate method to reduce the detection of oligomers. Please refer to Support & downloads -> Downloads -> Protocol booklet.
All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253) at 1/1000 dilution
Lane 1: Mouse brain with Alzheimer’s disease tissue lysate at 20 µg
Lane 2: Mouse brain with Alzheimer’s disease tissue lysate, membrane was incubated with alkaline phosphatase
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 18 kDa, 100 kDa
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