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Phospho Alpha-synuclein antibody pS129 [EP1536Y] ab209422 is a carrier free rabbit monoclonal antibody that is used in Alpha-synuclein western blotting, ELISA, IHC and Dot. Suitable for human, mouse and rat samples.

- PBS only, conjugation-ready, removing anything extra from your antibodies, greater flexibility in assay design
- Validated on the Leica BOND™ RX automated IHC staining platform for Alpha-synuclein IHC
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EP1536Y is cited in over 410 publications


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422), expandable thumbnail
  • Dot Blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422), expandable thumbnail
  • ELISA - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422), expandable thumbnail
  • Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422), expandable thumbnail
  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIHC-FrFlELISADotWBIPFlow CytIHC-Fr
Human
Tested
Expected
Tested
Tested
Expected
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Tested
Expected
Expected
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Expected
Expected
Expected
Tested
Not recommended
Not recommended
Not recommended
Chimpanzee
Not recommended
Predicted
Predicted
Predicted
Predicted
Not recommended
Not recommended
Not recommended
Cow
Not recommended
Predicted
Predicted
Predicted
Predicted
Not recommended
Not recommended
Not recommended
Gorilla
Not recommended
Predicted
Predicted
Predicted
Predicted
Not recommended
Not recommended
Not recommended
Macaque monkey
Not recommended
Predicted
Predicted
Predicted
Predicted
Not recommended
Not recommended
Not recommended
Orangutan
Not recommended
Predicted
Predicted
Predicted
Predicted
Not recommended
Not recommended
Not recommended
Pig
Not recommended
Predicted
Predicted
Predicted
Predicted
Not recommended
Not recommended
Not recommended
Spider monkey
Not recommended
Predicted
Predicted
Predicted
Predicted
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Orangutan

Dilution info

-

Notes

-

Species

Pig

Dilution info

-

Notes

-

Species

Chimpanzee

Dilution info

-

Notes

-

Species

Macaque monkey

Dilution info

-

Notes

-

Species

Gorilla

Dilution info

-

Notes

-

Species

Cow

Dilution info

-

Notes

-

Species

Spider monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Orangutan, Pig, Chimpanzee, Macaque monkey, Gorilla, Cow, Spider monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Orangutan, Pig, Chimpanzee, Macaque monkey, Gorilla, Cow, Spider monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Orangutan, Pig, Chimpanzee, Macaque monkey, Gorilla, Cow, Spider monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse, Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Orangutan, Pig, Chimpanzee, Macaque monkey, Gorilla, Cow, Spider monkey

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Orangutan, Pig, Chimpanzee, Macaque monkey, Gorilla, Cow, Spider monkey

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Orangutan, Pig, Chimpanzee, Macaque monkey, Gorilla, Cow, Spider monkey

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Orangutan, Pig, Chimpanzee, Macaque monkey, Gorilla, Cow, Spider monkey

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

2 products for Alternative Version

Target data

Function

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:20798282, PubMed:26442590, PubMed:28288128, PubMed:30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).

Alternative names

Recommended products

Phospho Alpha-synuclein antibody pS129 [EP1536Y] ab209422 is a carrier free rabbit monoclonal antibody that is used in Alpha-synuclein western blotting, ELISA, IHC and Dot. Suitable for human, mouse and rat samples.

- PBS only, conjugation-ready, removing anything extra from your antibodies, greater flexibility in assay design
- Validated on the Leica BOND™ RX automated IHC staining platform for Alpha-synuclein IHC
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EP1536Y is cited in over 410 publications

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EP1536Y

Purification technique

Affinity purification Protein A

Specificity

This antibody only detects alpha synuclein phosphorylated on Ser129. IHC-P: This antibody showed no staining in human hippocampus normal brain and showed staining in Parkinson's brain as expected.

Mouse and rat species are recommended based on WB results, we do not guarantee IHC-FrFl and IHC-P for Mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab209422 is the carrier-free version of Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Associated diseases and disorders

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422)

    This IHC data was generated using the same anti-phospho alpha synuclein S129 antibody clone, EP1536Y, in a different buffer formulation (cat# Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253).

    IHC image of alpha Synuclein (phospho S129) staining in Human Parkinson Substantia Nigra formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay performed on Human normal Substantia Nigra.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Dot Blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422), expandable thumbnail

    Dot Blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422)

    Dot blot analysis of alpha Synuclein (pS129) peptide (Lane 1), alpha Synuclein (unmodified) peptide (Lane 2) labelling alpha Synuclein (pS129) with Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253).

  • ELISA - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422), expandable thumbnail

    ELISA - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422)

    This ELISA data was generated using the same anti-phospho alpha synuclein S129 antibody clone, EP1536Y, in a different buffer formulation (cat# Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253).

    Direct ELISA antibody dose-response curve using Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253. Antibody concentration of 0-5000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

  • Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422)

    This data was developed using Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253, the same antibody clone in a different buffer formulation.

    Immunohistochemistry image of Alpha Synuclein Phospho S129 staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex* performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422), expandable thumbnail

    Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422)

    This data was developed using Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253, the same antibody clone in a different buffer.


    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This blot was developed using a higher sensitivity ECL substrate.

    Band around 100kda corresponds to αS oligomer (PMID: 27637918, PMID: 12597857)

    Lysates used here were prepared using RIPA method. We recommend 1% SDS hot lysate method to reduce the detection of oligomers. Please refer to Support & downloads -> Downloads -> Protocol booklet.

    All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253) at 1/1000 dilution

    Lane 1: Mouse brain with Alzheimer’s disease tissue lysate at 20 µg

    Lane 2: Mouse brain with Alzheimer’s disease tissue lysate, membrane was incubated with alkaline phosphatase

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa

    Observed band size: 18 kDa, 100 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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