AB209422

Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free

Name: Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - Carrier free (ab209422) | Abcam
Brand: Abcam Limited
SKU: AB209422
Price: 905 USD
Availability: InStock
Rating: 5 (1 reviews)

BOND RX™ Validated
RabMAb
Recombinant
What is this?

(1 Review)

(24 Publications)

Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422) is a rabbit recombinant monoclonal antibody in a PBS only buffer for easy conjugation. Suitable for Western Blot, IHC-P, IHC-FrFl, ELISA, Dot Blot in Human, Mouse, Rat.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422)

This IHC data was generated using the same anti-phospho alpha synuclein S129 antibody clone, EP1536Y, in a different buffer formulation (cat# ab51253).

IHC image of alpha Synuclein (phospho S129) staining in Human Parkinson Substantia Nigra formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51253, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay performed on Human normal Substantia Nigra.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422)

This data was developed using ab51253, the same antibody clone in a different buffer formulation.

Immunohistochemistry image of Alpha Synuclein Phospho S129 staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex* performed on a Leica Biosystems BOND^® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab51253, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Unknown

Dot Blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422)

Dot blot analysis of alpha Synuclein (pS129) peptide (Lane 1), alpha Synuclein (unmodified) peptide (Lane 2) labelling alpha Synuclein (pS129) with ab51253 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51253).

Supplier Data

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422)

This data was developed using ab51253, the same antibody clone in a different buffer.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a higher sensitivity ECL substrate.

Band around 100kda corresponds to αS oligomer (PMID : 27637918, PMID : 12597857)

Lysates used here were prepared using RIPA method. We recommend 1% SDS hot lysate method to reduce the detection of oligomers. Please refer to Support & downloads -> Downloads -> Protocol booklet.

All lanes:

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (<a href='/en-us/products/primary-antibodies/alpha-synuclein-phospho-s129-antibody-ep1536y-ab51253'>ab51253</a>) at 1/1000 dilution

Lane 1:

Mouse brain with Alzheimer's disease tissue lysate at 20 µg

Lane 2:

Mouse brain with Alzheimer's disease tissue lysate, membrane was incubated with alkaline phosphatase

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 14 kDa

Observed band size: 18 kDa,100 kDa

false

Lab

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate. We suggest optimizing experimental protocols (increasing lysate amount, using lower antibody dilution or higher sensitivity ECL substrate) to improve results.

All lanes:

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa

true

Exposure time: 180s

ELISA - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422)

ELISA

Unknown

ELISA - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422)

This ELISA data was generated using the same anti-phospho alpha synuclein S129 antibody clone, EP1536Y, in a different buffer formulation (cat# ab51253).

Direct ELISA antibody dose-response curve using ab51253. Antibody concentration of 0-5000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

Unconjugated

Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1536Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-FrFl, WB, Dot, ELISA, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects alpha synuclein phosphorylated on Ser129. IHC-P: This antibody showed no staining in human hippocampus normal brain and showed staining in Parkinson's brain as expected.

Mouse and rat species are recommended based on WB results, we do not guarantee IHC-P for mouse and rat.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (ab209422) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), ELISA, Dot Blot in Human, Mouse, Rat samples.

What is the molecular weight of Alpha-synuclein?
Anti-Alpha-synuclein (phospho S129) [EP1536Y] - BSA and Azide free (ab209422) specifically detects a band for Alpha-synuclein (UniProt: P37840) at a molecular weight of 14kDa.

Trusted by the scientific community
Anti-Alpha-synuclein (phospho S129) [EP1536Y] - BSA and Azide free (ab209422) was first used in a scientific publication in 2016 and has been cited over 20 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [EP1536Y] also available for your convenience: ab51253, Carrier free - ab209422, Alexa Fluor® 594 - ab282652, Alexa Fluor® 568 - ab307166, Alexa Fluor® 488 - ab310949, Alexa Fluor® 647 - ab311067, Alexa Fluor® 555 - ab313137, Alexa Fluor® 750 - ab321645

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed : 20798282, PubMed : 26442590, PubMed : 28288128, PubMed : 30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed : 28288128, PubMed : 30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed : 30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed : 20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed : 26442590).

See full target information SNCA phospho S129

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2410229 PubMed39804980

2025

Molecular Determinants of Protein Pathogenicity at the Single-Aggregate Level.

Applications

Unspecified application

Species

Unspecified reactive species

Agnieszka Urbanek,Emma F Garland,Emily E Prescott,Marianne C King,Anna Olerinyova,Hollie E Wareing,Nia Georgieva,Ellie L Bradshaw,Svetomir B Tzokov,Alexander Knight,Alexander I Tartakovskii,Tarja Malm,J Robin Highley,Suman De

Scientific reports 14:16089 PubMed38997314

2024

Retinal hyperspectral imaging in mouse models of Parkinson's disease and healthy aging.

Applications

Unspecified application

Species

Unspecified reactive species

Paul Trlin,Jenny Gong,Katie K N Tran,Vickie H Y Wong,Pei Ying Lee,Anh Hoang,Da Zhao,Leah C Beauchamp,Jeremiah K H Lim,Andrew Metha,Kevin J Barnham,David I Finkelstein,Bang V Bui,Phillip Bedggood,Christine T O Nguyen

NPJ Parkinson's disease 9:120 PubMed37553379

2023

Parkinson's disease neurons exhibit alterations in mitochondrial quality control proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Chen,David McDonald,Alasdair Blain,Emily Mossman,Kiera Atkin,Michael F Marusich,Roderick Capaldi,Laura Bone,Anna Smith,Andrew Filby,Daniel Erskine,Oliver Russell,Gavin Hudson,Amy E Vincent,Amy K Reeve

Frontiers in neuroscience 17:1146979 PubMed37214398

2023

Retinal alpha-synuclein accumulation correlates with retinal dysfunction and structural thinning in the A53T mouse model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Katie K N Tran,Vickie H Y Wong,Anh Hoang,David I Finkelstein,Bang V Bui,Christine T O Nguyen

ACS chemical neuroscience 14:1238-1248 PubMed36920792

2023

Development of a Novel Electrochemiluminescence ELISA for Quantification of α-Synuclein Phosphorylated at Ser in Biological Samples.

Applications

Unspecified application

Species

Unspecified reactive species

Suman Dutta,Simon Hornung,Hash Brown Taha,Karl Biggs,Ibrar Siddique,Lea M Chamoun,Hedieh Shahpasand-Kroner,Carter Lantz,Marcos Herrera-Vaquero,Nadia Stefanova,Joseph A Loo,Gal Bitan

Acta neuropathologica communications 9:64 PubMed33832546

2021

Mixed pathologies in pancreatic β cells from subjects with neurodegenerative diseases and their interaction with prion protein.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan Martinez-Valbuena,Rafael Valenti-Azcarate,Irene Amat-Villegas,Irene Marcilla,Gloria Marti-Andres,Maria-Cristina Caballero,Mario Riverol,María-Teresa Tuñon,Paul E Fraser,María-Rosario Luquin

Acta neuropathologica 135:877-886 PubMed29536165

2018

Interaction of amyloidogenic proteins in pancreatic β cells from subjects with synucleinopathies.

Applications

IHC-P

Species

Human

Ivan Martinez-Valbuena,Irene Amat-Villegas,Rafael Valenti-Azcarate,Maria Del Mar Carmona-Abellan,Irene Marcilla,Maria-Teresa Tuñon,Maria-Rosario Luquin

Scientific reports 5:14211 PubMed26381815

2015

Sensitive western blotting for detection of endogenous Ser129-phosphorylated α-synuclein in intracellular and extracellular spaces.

Applications

Species

Human

Asuka Sasaki,Shigeki Arawaka,Hiroyasu Sato,Takeo Kato

Human molecular genetics 24:1061-76 PubMed25296918

2014

Potentiation of neurotoxicity in double-mutant mice with Pink1 ablation and A53T-SNCA overexpression.

Applications

WB, IHC-P, IHC-FrFl

Species

Mouse, Mouse, Mouse

Suzana Gispert,Nadine Brehm,Jonas Weil,Kay Seidel,Udo Rüb,Beatrice Kern,Michael Walter,Jochen Roeper,Georg Auburger

Proceedings of the National Academy of Sciences of 111:E3976-85 PubMed25201965

2014

Phosphatidylethanolamine deficiency disrupts α-synuclein homeostasis in yeast and worm models of Parkinson disease.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoxiao Wang,Siyuan Zhang,Liang-Chun Liou,Qun Ren,Zhaojie Zhang,Guy A Caldwell,Kim A Caldwell,Stephan N Witt

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Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422)

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422)

Dot Blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422)

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422)

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422)

ELISA - Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free (AB209422)

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