AB168381

Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)]

Name: Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381) | Abcam
Brand: Abcam Limited
SKU: AB168381
Price: 185 USD
Availability: InStock
Rating: 5 (5 reviews)

BOND RX™ Validated
RabMAb
Recombinant
20ul selling size
What is this?

(5 Reviews)

(53 Publications)

Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381) is a rabbit monoclonal antibody detecting Alpha-synuclein in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 40 publications

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

5 Images

Supplier Data

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (AB168381)

Blocking and Diluting buffer and concentration - 5% NFDM/TBST

Exposure time : 5 seconds

All lanes:

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)

Lane 1:

In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of PLK2 (Polo-like kinase 2) but absence of ATP

Lane 2:

In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of ATP but absence of PLK2 (Polo-like kinase 2)

Lane 3:

In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of PLK2 (Polo-like kinase 2) and ATP

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 14 kDa

Observed band size: 17 kDa

false

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (AB168381)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (AB168381)

Immunohistochemistry image of Alpha Synuclein Phospho S129 staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex* performed on a Leica Biosystems BOND^® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab168381, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen.The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (AB168381)

Immunohistochemistry image of Alpha Synuclein Phospho S129 staining in a section of formalin-fixed paraffin-embedded normal human Parkinson's brain* performed on a Leica Biosystems BOND^® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab168381, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

AbReview62634****

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (AB168381)

Lysate prepared in PBS + 1% Triton X-100. Membrane fixed with 0.4% PFA in PBS for 30 min prior to blocking.

Primary incubation for 12 hours at 4°C.

All lanes:

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381) at 1/2000 dilution

All lanes:

Mouse cortical neuron lysate at 30 µg

Secondary

All lanes:

Goat anti-rabbit IgG (H+L) HRP

Predicted band size: 14 kDa

true

Exposure time: 5min

Image courtesy of Mr. Chun Chau SUNG

Collaborator

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (AB168381)

All lanes:

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381) at 1/1000 dilution

Lane 1:

Recombinant alpha Synuclein expressed in BL21 bacterial cells

Lane 2:

Recombinant alpha Synuclein expressed in BL21 bacterial cells, in the presence of Human Polo-Like Kinase 2

Lane 3:

HEK whole cell lysates, stably-transfected with Polo-Like Kinase 2 and alpha Synuclein

Predicted band size: 14 kDa

false

This image is courtesy of Drs. Shyra Gardai and Jennifer Johnston (Elan Pharmaceuticals)

Carrier free

Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MJF-R13 (8-8)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects alpha Synuclein phosphorylated on Ser129.

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Reactivity data

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Product details

Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P, WB in human samples.

Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381) has been cited over 40 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-Alpha-synuclein (phospho S129) antibody (Anti-pS129 alpha-synuclein antibody) [MJF-R13 (8-8)] (ab168381) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381) has 4 independent reviews from customers.

Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381) specifically detects Alpha-synuclein Phospho-S129 (UniProt ID: P37840; Molecular weight: 14kDa) and is sold in 100 µL and 1 mL selling sizes.

Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381) was developed by Abcam in partnership with the Michael J. Fox Foundation for Parkinson's Research.

Alpha-synuclein phosphorylated at serine 129 (pS129; SNCA/NACP) is a key pathological marker in neurodegenerative diseases such as Parkinson's disease. This phosphorylation event is associated with the abnormal aggregation of alpha-synuclein into Lewy bodies, which are indicative of disease. Studies have shown that pS129 not only promotes the aggregation of alpha-synuclein but also increases its neurotoxicity, contributing to neuronal death and disease progression

Alpha-synuclein was the first gene to be linked to Parkinson's disease (PD) and remains the most promising link to PD pathogenesis, where there is genetic evidence that it may play a causal role. In the brain, alpha-synuclein is concentrated in presynaptic nerve terminals. The deposition of the abundant presynaptic brain protein alpha-synuclein as fibrillary aggregates in neurons or glial cells is a hallmark lesion in a subset of neurodegenerative disorders. These disorders include Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy, collectively referred to as synucleinopathies. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin.
Recent studies also indicate that alpha-synuclein undergoes post-translational modification. Though the role of many of these modifications is still under investigation, phosphorylation at Serine 129 may affect alpha-synuclein aggregations and may also serve as marker of disease pathogenesis. With the advent of this phospho-specific Serine 129 antibody, The Michael J. Fox Foundation hopes to ensure that the putative role of this modification can be further examined by all researchers.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed : 20798282, PubMed : 26442590, PubMed : 28288128, PubMed : 30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed : 28288128, PubMed : 30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed : 30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed : 20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed : 26442590).

See full target information SNCA phospho S129

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Publications (53)

Recent publications for all applications. Explore the full list and refine your search

NPJ Parkinson's disease 11:232 PubMed40775228

2025

Phosphorylated α-synuclein in CSF and plasma does not reflect synucleinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni Bellomo,Erik Stoops,Jeroen Vanbrabant,Leentje Demeyer,Cindy Francois,Melanie Vanhooren,Yihua Ma,Carly M Farris,Luis Concha-Marambio,Federico Paolini Paoletti,Lorenzo Gaetani,Lucilla Parnetti,Davide Chiasserini

Nature neuroscience 28:925-936 PubMed40082617

2025

Sympathetic and parasympathetic subtypes of body-first Lewy body disease observed in postmortem tissue from prediagnostic individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Katrine B Andersen,Anushree Krishnamurthy,Mie Kristine Just,Nathalie Van Den Berge,Casper Skjærbæk,Jacob Horsager,Karoline Knudsen,Jacob W Vogel,Jon B Toledo,Johannes Attems,Tuomo Polvikoski,Yuko Saito,Shigeo Murayama,Per Borghammer

Acta neuropathologica communications 13:14 PubMed39838428

2025

Distinct subcellular localization of tau and alpha-synuclein in lewy body disease.

Applications

Unspecified application

Species

Unspecified reactive species

D Luke Fischer,Marissa Menard,Omar Z Abdelaziz,Nicholas M Kanaan,Virginia G Cobbs,Richard E Kennedy,Geidy E Serrano,Thomas G Beach,Laura A Volpicelli-Daley

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2410229 PubMed39804980

2025

Molecular Determinants of Protein Pathogenicity at the Single-Aggregate Level.

Applications

Unspecified application

Species

Unspecified reactive species

Agnieszka Urbanek,Emma F Garland,Emily E Prescott,Marianne C King,Anna Olerinyova,Hollie E Wareing,Nia Georgieva,Ellie L Bradshaw,Svetomir B Tzokov,Alexander Knight,Alexander I Tartakovskii,Tarja Malm,J Robin Highley,Suman De

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e70121 PubMed39436202

2024

The role of polo-like kinases 2 in the proteasomal and lysosomal degradation of alpha-synuclein in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Chau Sung,Wai Yun Lam,Kenny K K Chung

Bioanalysis 16:1125-1139 PubMed39404180

2024

An immunoassay for the quantification of phosphorylated α-synuclein at serine 129 in human cerebrospinal fluid.

Applications

Unspecified application

Species

Unspecified reactive species

Achani M Silva,Elizabeth S Hickford,Paul Cutler

Cell reports. Medicine 5:101570 PubMed38749422

2024

SARS-CoV-2 infection exacerbates the cellular pathology of Parkinson's disease in human dopaminergic neurons and a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Bina Lee,Ha Nyeoung Choi,Young Hyun Che,Myungjun Ko,Hye Min Seong,Min Gi Jo,Seon-Hee Kim,Chieun Song,Subeen Yoon,Jiwoo Choi,Jeong Hee Kim,Minkyeong Kim,Min Young Lee,Sang Won Park,Hye Jung Kim,Seong Jae Kim,Do Sik Moon,Sun Lee,Jae-Hoon Park,Seung-Geun Yeo,Richard G Everson,Young Jin Kim,Kyung-Wook Hong,In-Soon Roh,Kwang-Soo Lyoo,Yong Jun Kim,Seung Pil Yun

Nature communications 15:2436 PubMed38499535

2024

HTRA1 disaggregates α-synuclein amyloid fibrils and converts them into non-toxic and seeding incompetent species.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Chen,Anuradhika Puri,Braxton Bell,Joseph Fritsche,Hector H Palacios,Maurie Balch,Macy L Sprunger,Matthew K Howard,Jeremy J Ryan,Jessica N Haines,Gary J Patti,Albert A Davis,Meredith E Jackrel

Scientific reports 13:22613 PubMed38114623

2023

Impaired neuronal activity as a potential factor contributing to the underdeveloped cerebrovasculature in a young Parkinson's disease mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Young Jeong,Hyun Jung Lee,Namsuk Kim,Yan Li,Jong-Cheol Rah,Won-Jong Oh

Neurobiology of disease 183:106166 PubMed37245833

2023

Stabilization of 14-3-3 protein-protein interactions with Fusicoccin-A decreases alpha-synuclein dependent cell-autonomous death in neuronal and mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Rodrigo Vinueza-Gavilanes,Jorge Juan Bravo-González,Leyre Basurco,Chiara Boncristiani,Joaquín Fernández-Irigoyen,Enrique Santamaría,Irene Marcilla,Alberto Pérez-Mediavilla,María Rosario Luquin,Africa Vales,Gloria González-Aseguinolaza,María Soledad Aymerich,Tomás Aragón,Montserrat Arrasate

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)]

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (AB168381)

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (AB168381)

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (AB168381)

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (AB168381)

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (AB168381)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (53)

Product promise