Skip to main content

Rabbit Recombinant Monoclonal Alpha-synuclein phospho S129 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 40 publications.


Images

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (AB168381), expandable thumbnail
  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (AB168381), expandable thumbnail
  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (AB168381), expandable thumbnail
  • Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (AB168381), expandable thumbnail
  • Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (AB168381), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Not recommended
Not recommended
Tested
Not recommended
Tested

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.5 µg/mL

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:20798282, PubMed:26442590, PubMed:28288128, PubMed:30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Alpha-synuclein phospho S129 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 40 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

MJF-R13 (8-8)

Purification technique

Affinity purification Protein A

Specificity

This antibody only detects alpha Synuclein phosphorylated on Ser129.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Alpha-synuclein was the first gene to be linked to Parkinson's disease (PD) and remains the most promising link to PD pathogenesis, where there is genetic evidence that it may play a causal role. In the brain, alpha-synuclein is concentrated in presynaptic nerve terminals. The deposition of the abundant presynaptic brain protein alpha-synuclein as fibrillary aggregates in neurons or glial cells is a hallmark lesion in a subset of neurodegenerative disorders. These disorders include Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy, collectively referred to as synucleinopathies. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin.
Recent studies also indicate that alpha-synuclein undergoes post-translational modification. Though the role of many of these modifications is still under investigation, phosphorylation at Serine 129 may affect alpha-synuclein aggregations and may also serve as marker of disease pathogenesis. With the advent of this phospho-specific Serine 129 antibody, The Michael J. Fox Foundation hopes to ensure that the putative role of this modification can be further examined by all researchers.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody was developed with support from The Michael J. Fox Foundation.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Associated diseases and disorders

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381), expandable thumbnail
    Image courtesy of Mr. Chun Chau SUNG

    Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)

    Lysate prepared in PBS + 1% Triton X-100. Membrane fixed with 0.4% PFA in PBS for 30 min prior to blocking.

    Primary incubation for 12 hours at 4°C.

    All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381) at 1/2000 dilution

    All lanes: Mouse cortical neuron lysate at 30 µg

    Secondary

    All lanes: Goat anti-rabbit IgG (H+L) HRP

    Developed using the ECL technique.

    Predicted band size: 14 kDa

    Exposure time: 5min

  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381), expandable thumbnail

    Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)

    Blocking and Diluting buffer and concentration - 5% NFDM/TBST

    Exposure time: 5 seconds

    All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)

    Lane 1: In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of PLK2 (Polo-like kinase 2) but absence of ATP

    Lane 2: In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of ATP but absence of PLK2 (Polo-like kinase 2)

    Lane 3: In vitro kinase assay of Alpha Synuclein phosphorylation using His tagged human full length recombinant alpha-synuclein protein in the presence of PLK2 (Polo-like kinase 2) and ATP

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa

    Observed band size: 17 kDa

  • Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381), expandable thumbnail
    This image is courtesy of Drs. Shyra Gardai and Jennifer Johnston (Elan Pharmaceuticals)

    Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)

    All lanes: Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381) at 1/1000 dilution

    Lane 1: Recombinant alpha Synuclein expressed in BL21 bacterial cells

    Lane 2: Recombinant alpha Synuclein expressed in BL21 bacterial cells, in the presence of Human Polo-Like Kinase 2

    Lane 3: HEK whole cell lysates, stably-transfected with Polo-Like Kinase 2 and alpha Synuclein

    Predicted band size: 14 kDa

  • Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)

    Immunohistochemistry image of Alpha Synuclein Phospho S129 staining in a section of formalin-fixed paraffin-embedded normal human Parkinson's brain* performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab168381, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (ab168381)

    Immunohistochemistry image of Alpha Synuclein Phospho S129 staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex* performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab168381, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen.The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com