Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] - BSA and Azide free (AB209421)

This data was developed using ab168381, the same antibody clone in a different buffer formulation.

Immunohistochemistry image of Alpha Synuclein Phospho S129 staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex* performed on a Leica Biosystems BOND^® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab168381, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.The inset secondary-only control image is taken from an identical assay without primary antibody.

For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

• IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] - BSA and Azide free (AB209421)

This data was developed using ab168381, the same antibody clone in a different buffer formulation.

Immunohistochemistry image of Alpha Synuclein Phospho S129 staining in a section of formalin-fixed paraffin-embedded normal human Parkinson's brain* performed on a Leica Biosystems BOND^® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab168381, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

Collaborator

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] - BSA and Azide free (AB209421)

This data was developed using ab168381, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Alpha-synuclein (phospho S129) antibody [MJF-R13 (8-8)] (<a href='/en-us/products/primary-antibodies/alpha-synuclein-phospho-s129-antibody-mjf-r13-8-8-ab168381'>ab168381</a>) at 1/1000 dilution

Lane 1:

Recombinant alpha Synuclein expressed in BL21 bacterial cells

Lane 2:

Recombinant alpha Synuclein expressed in BL21 bacterial cells, in the presence of Human Polo-Like Kinase 2

Lane 3:

HEK whole cell lysates, stably-transfected with Polo-Like Kinase 2 and alpha Synuclein

Predicted band size: 14 kDa

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This image is courtesy of Drs. Shyra Gardai and Jennifer Johnston (Elan Pharmaceuticals)