AB184674

Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A]

Name: Anti-alpha-synuclein (phospho S129) antibody [P-syn/81A] (ab184674) | Abcam
Brand: Abcam Limited
SKU: AB184674
Price: 470 USD
Availability: InStock
Rating: 4 (2 reviews)

BOND RX™ Validated
What is this?

(2 Reviews)

(31 Publications)

Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (ab184674) is a mouse monoclonal antibody detecting Alpha-synuclein in IHC-P. Suitable for Human.

- Over 30 publications

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (AB184674)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (AB184674)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded diseased human brain tissue labeling alpha Synuclein (phospho S129) with ab184674 at 1/500 dilution.

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (AB184674)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (AB184674)

Immunohistochemistry image of Alpha Synuclein Phospho S129 staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex* performed on a Leica Biosystems BOND^® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab184674, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

P-syn/81A

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human SNCA phospho S129 aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P37840

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Retrieve antigens with 70% Formic acid for 10-30 minutes at room temperature before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (ab184674) is a mouse monoclonal antibody and is validated for use in IHC-P in human, mouse, rat samples.

Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (ab184674) has been cited over 31 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (ab184674) has high sensitivity and specificity.

Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (ab184674) specifically detects Alpha-synuclein Phospho-S129 (Phospho-α-synuclein Ser129) (UniProt ID: P37840; Molecular weight: 14kDa) and is sold in 50 µL selling sizes.

Alpha-synuclein phosphorylated at serine 129 (NACP/SNCA pS129) is a post-translational modification of the alpha-synuclein protein, which is involved in synaptic function. Elevated levels of pS129 are found in the brains of individuals with Parkinson's disease and related disorders. This modification is linked to the aggregation of alpha-synuclein into Lewy bodies, which are pathological features of these diseases. Research suggests that pS129 may enhance the neurotoxic effects of alpha-synuclein, playing a critical role in the development and progression of neurodegenerative conditions.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

Preservative: 0.09% Sodium azide Constituents: 99.91% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.

See full target information SNCA phospho S129

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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:7295 PubMed37957154

2023

Knockout or inhibition of USP30 protects dopaminergic neurons in a Parkinson's disease mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Tracy-Shi Zhang Fang,Yu Sun,Andrew C Pearce,Simona Eleuteri,Mark Kemp,Christopher A Luckhurst,Rachel Williams,Ross Mills,Sarah Almond,Laura Burzynski,Nóra M Márkus,Christopher J Lelliott,Natasha A Karp,David J Adams,Stephen P Jackson,Jin-Feng Zhao,Ian G Ganley,Paul W Thompson,Gabriel Balmus,David K Simon

Cells 12: PubMed37048156

2023

α-Synuclein Preformed Fibrils Bind to β-Neurexins and Impair β-Neurexin-Mediated Presynaptic Organization.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Feller,Aurélie Fallon,Wen Luo,Phuong Trang Nguyen,Irina Shlaifer,Alfred Kihoon Lee,Nicolas Chofflet,Nayoung Yi,Husam Khaled,Samer Karkout,Steve Bourgault,Thomas M Durcan,Hideto Takahashi

NPJ Parkinson's disease 9:44 PubMed36973269

2023

The small GTPase Rit2 modulates LRRK2 kinase activity, is required for lysosomal function and protects against alpha-synuclein neuropathology.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Obergasteiger,Anne-Marie Castonguay,Sara Pizzi,Stefano Magnabosco,Giulia Frapporti,Evy Lobbestael,Veerle Baekelandt,Andrew A Hicks,Peter P Pramstaller,Claude Gravel,Corrado Corti,Martin Lévesque,Mattia Volta

Methods in molecular biology (Clifton, N.J.) 2551:357-378 PubMed36310215

2022

Propagation of Distinct α-Synuclein Strains Within Human Reconstructed Neuronal Network and Associated Neuronal Dysfunctions.

Applications

Unspecified application

Species

Unspecified reactive species

Simona Gribaudo,Luc Bousset,Josquin Courte,Ronald Melki,Jean-Michel Peyrin,Anselme L Perrier

Acta neuropathologica 144:881-910 PubMed36121476

2022

Stress-inducible phosphoprotein 1 (HOP/STI1/STIP1) regulates the accumulation and toxicity of α-synuclein in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel E Lackie,Aline S de Miranda,Mei Peng Lim,Vladislav Novikov,Nimrod Madrer,Nadun C Karunatilleke,Benjamin S Rutledge,Stephanie Tullo,Anne Brickenden,Matthew E R Maitland,David Greenberg,Daniel Gallino,Wen Luo,Anoosha Attaran,Irina Shlaifer,Esther Del Cid Pellitero,Caroline Schild-Poulter,Thomas M Durcan,Edward A Fon,Martin Duennwald,Flavio H Beraldo,M Mallar Chakravarty,Timothy J Bussey,Lisa M Saksida,Hermona Soreq,Wing-Yiu Choy,Vania F Prado,Marco A M Prado

Cell death & disease 13:575 PubMed35773260

2022

p21-activated kinase 4 controls the aggregation of α-synuclein by reducing the monomeric and aggregated forms of α-synuclein: involvement of the E3 ubiquitin ligase NEDD4-1.

Applications

Unspecified application

Species

Unspecified reactive species

So-Yoon Won,Jung-Jin Park,Soon-Tae You,Jong-A Hyeun,Hyong-Kyu Kim,Byung Kwan Jin,Catriona McLean,Eun-Young Shin,Eung-Gook Kim

Movement disorders : official journal of the Movement Disorder Society 37:1739-1748 PubMed35524682

2022

Dopaminergic Positron Emission Tomography Imaging in the Alpha-Synuclein Preformed Fibril Model Reveals Similarities to Early Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Vesna Sossi,Joseph R Patterson,Siobhan McCormick,Christopher J Kemp,Kathryn M Miller,Anna C Stoll,Nathan Kuhn,Michael Kubik,Joseph Kochmanski,Kelvin C Luk,Caryl E Sortwell

Acta neuropathologica communications 9:189 PubMed34819144

2021

Assembly of α-synuclein and neurodegeneration in the central nervous system of heterozygous M83 mice following the peripheral administration of α-synuclein seeds.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer A Macdonald,John L Chen,Masami Masuda-Suzukake,Manuel Schweighauser,Zane Jaunmuktane,Thomas Warner,Janice L Holton,Annabelle Grossman,Richard Berks,Isabelle Lavenir,Michel Goedert

Nature communications 12:5907 PubMed34625559

2021

Modeling alpha-synuclein pathology in a human brain-chip to assess blood-brain barrier disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Iosif Pediaditakis,Konstantia R Kodella,Dimitris V Manatakis,Christopher Y Le,Chris D Hinojosa,William Tien-Street,Elias S Manolakos,Kostas Vekrellis,Geraldine A Hamilton,Lorna Ewart,Lee L Rubin,Katia Karalis

Journal of cellular and molecular medicine 25:9634-9646 PubMed34486218

2021

Prolyl oligopeptidase inhibition reduces alpha-synuclein aggregation in a cellular model of multiple system atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Hengjing Cui,Tommi Kilpeläinen,Lydia Zouzoula,Samuli Auno,Kalevi Trontti,Sampo Kurvonen,Susanna Norrbacka,Iiris Hovatta,Poul Henning Jensen,Timo T Myöhänen

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (AB184674)

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (AB184674)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (31)

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