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AB184674

Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A]

4

(2 Reviews)

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(51 Publications)

Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (ab184674) is a mouse monoclonal antibody detecting Alpha-synuclein in IHC-P. Suitable for Human.

- Over 30 publications

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (AB184674)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (AB184674)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded diseased human brain tissue labeling alpha Synuclein (phospho S129) with ab184674 at 1/500 dilution.

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (AB184674)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (AB184674)

Immunohistochemistry image of Alpha Synuclein Phospho S129 staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex* performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab184674, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

P-syn/81A

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human SNCA phospho S129 aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P37840

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Retrieve antigens with 70% Formic acid for 10-30 minutes at room temperature before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (ab184674) is a mouse monoclonal antibody and is validated for use in IHC-P in human, mouse, rat samples.

Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (ab184674) has been cited over 31 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (ab184674) has high sensitivity and specificity.

Anti-Alpha-synuclein (phospho S129) antibody [P-syn/81A] (ab184674) specifically detects Alpha-synuclein Phospho-S129 (Phospho-α-synuclein Ser129) (UniProt ID: P37840; Molecular weight: 14kDa) and is sold in 50 µL selling sizes.

Alpha-synuclein phosphorylated at serine 129 (NACP/SNCA pS129) is a post-translational modification of the alpha-synuclein protein, which is involved in synaptic function. Elevated levels of pS129 are found in the brains of individuals with Parkinson's disease and related disorders. This modification is linked to the aggregation of alpha-synuclein into Lewy bodies, which are pathological features of these diseases. Research suggests that pS129 may enhance the neurotoxic effects of alpha-synuclein, playing a critical role in the development and progression of neurodegenerative conditions.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed : 20798282, PubMed : 26442590, PubMed : 28288128, PubMed : 30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed : 28288128, PubMed : 30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed : 30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed : 20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed : 26442590).
See full target information SNCA phospho S129
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Publications (51)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:26537 PubMed40691198

2025

Templating of monomeric alpha-synuclein results in inflammation and SNpc dopamine neuron death in a genetic mouse model of induced synucleinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew D Byrne,Peyman Petramfar,Jae-Kyung Lee,Richard Jay Smeyne

Translational neurodegeneration 14:31 PubMed40537859

2025

Pathological α-synuclein elicits granulovacuolar degeneration independent of tau.

Applications

Unspecified application

Species

Unspecified reactive species

Dylan J Dues,Madalynn L Erb,Alysa Kasen,Naman Vatsa,Erin T Williams,An Phu Tran Nguyen,Michael X Henderson,Darren J Moore

Frontiers in neuroscience 19:1556447 PubMed40236948

2025

Intrastriatal injection of alpha-synuclein preformed fibrils to rats results in L-DOPA reversible sensorimotor impairments and alterations in non-motor function.

Applications

Unspecified application

Species

Unspecified reactive species

Sheila M Fleming,Sophia Scott,Edward J Hamad,Danielle E Herman,John G Holden,Lily Yan,Katrina Linning-Duffy,Christopher J Kemp,Joseph R Patterson,Kathryn M Miller,Michael Kubik,Nathan Kuhn,Anna C Stoll,Megan F Duffy,Kathy Steece-Collier,Allyson Cole-Strauss,Jack W Lipton,Kelvin C Luk,Caryl E Sortwell

NPJ Parkinson's disease 11:61 PubMed40155632

2025

The homozygous LRRK2.p.N1437D point mutation mouse is a novel model of parkinsonism.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Hua Gan,Yi-Min Sun,Xin-Yue Zhou,Zhi-Yuan Qi,Feng-Tao Liu,Yi-Lin Tang,Wen-Bo Yu,Bao-Guo Xiao,Jian Wang,Jian-Jun Wu

Life medicine 3:lnae011 PubMed39872397

2025

Tracing TMEM106B fibril deposition in aging and Parkinson's disease with dementia brains.

Applications

Unspecified application

Species

Unspecified reactive species

Wanbing Zhao,Yun Fan,Qinyue Zhao,Zhen Fan,Jue Zhao,Wenbo Yu,Wensheng Li,Dan Li,Cong Liu,Jian Wang

Translational neurodegeneration 13:67 PubMed39726015

2024

N-terminus α-synuclein detection reveals new and more diverse aggregate morphologies in multiple system atrophy and Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

James A Wiseman,YuHong Fu,Richard L M Faull,Clinton P Turner,Maurice A Curtis,Glenda M Halliday,Birger V Dieriks

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2406700 PubMed39721018

2024

OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomeng Song,Tengfei Liu,Lu Yu,Qiuran Ji,Xin Guo,Runzhe Zong,Yiquan Li,Gan Huang,Qidi Xue,Qingyi Fu,Bingyu Liu,Yi Zheng,Lin Chen,Chengjiang Gao,Huiqing Liu

NPJ Parkinson's disease 10:194 PubMed39443520

2024

CRBN modulates synuclein fibrillation via degradation of DNAJB1 in mouse model of Parkinson disease.

Applications

Unspecified application

Species

Unspecified reactive species

Uroos Akber,Jun-Hyung Jung,Heewoong Yoon,Jiwon Seo,Chul-Seung Park

Cell reports methods 4:100876 PubMed39413778

2024

An accelerated Parkinson's disease monkey model using AAV-α-synuclein plus poly(ADP-ribose).

Applications

Unspecified application

Species

Unspecified reactive species

Shuyi Liu,Naixue Yang,Yaping Yan,Shaobo Wang,Jialing Chen,Yichao Wang,Xue Gan,Jiawen Zhou,Guoqing Xie,Hong Wang,Tianzhuang Huang,Weizhi Ji,Zhengbo Wang,Wei Si

Bioanalysis 16:1125-1139 PubMed39404180

2024

An immunoassay for the quantification of phosphorylated α-synuclein at serine 129 in human cerebrospinal fluid.

Applications

Unspecified application

Species

Unspecified reactive species

Achani M Silva,Elizabeth S Hickford,Paul Cutler
View all publications
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