AB131466

Anti-Alpha-synuclein (phospho Y125) antibody

Name: Anti-Alpha-synuclein (phospho Y125) antibody (ab131466) | Abcam
Brand: Abcam Limited
SKU: AB131466
Price: 520 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Alpha-synuclein phospho Y125 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SNCA phospho Y125 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein (phospho Y125) antibody (AB131466)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein (phospho Y125) antibody (AB131466)

Immunoflourescent analysis of alpha Synuclein (phospho Y125)in methanol fixed HeLa cells stained with ab131466 at a 1/100 dilution.

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Western blot - Anti-Alpha-synuclein (phospho Y125) antibody (AB131466)

All lanes:

Western blot - Anti-Alpha-synuclein (phospho Y125) antibody (ab131466) at 1/500 dilution

Lane 1:

Extract from C2C12 cells, untreated

Lane 2:

Extract from C2C12 cells treated with Anisomycin

Predicted band size: 14 kDa

Observed band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human SNCA phospho Y125 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P37840

Specificity

ab131466 detects alpha Synuclein only when phosphorylated at tyrosine 125.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab131466 was purified by affinity- chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.

See full target information SNCA phospho Y125

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

NPJ Parkinson's disease 9:4 PubMed36646701

2023

Dynamic physiological α-synuclein S129 phosphorylation is driven by neuronal activity.

Applications

Unspecified application

Species

Unspecified reactive species

Nagendran Ramalingam,Shan-Xue Jin,Tim E Moors,Luis Fonseca-Ornelas,Kazuma Shimanaka,Shi Lei,Hugh P Cam,Aurelia Hays Watson,Lisa Brontesi,Lai Ding,Dinc Yasat Hacibaloglu,Haiyang Jiang,Se Joon Choi,Ellen Kanter,Lei Liu,Tim Bartels,Silke Nuber,David Sulzer,Eugene V Mosharov,Weisheng V Chen,Shaomin Li,Dennis J Selkoe,Ulf Dettmer

Scientific reports 12:17446 PubMed36261683

2022

Elk-1 regulates retinal ganglion cell axon regeneration after injury.

Applications

Unspecified application

Species

Unspecified reactive species

Takahiko Noro,Sahil H Shah,Yuqin Yin,Riki Kawaguchi,Satoshi Yokota,Kun-Che Chang,Ankush Madaan,Catalina Sun,Giovanni Coppola,Daniel Geschwind,Larry I Benowitz,Jeffrey L Goldberg

Brain pathology (Zurich, Switzerland) 30:831-843 PubMed32324926

2020

Investigating the presence of doubly phosphorylated α-synuclein at tyrosine 125 and serine 129 in idiopathic Lewy body diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Muneera Fayyad,Daniel Erskine,Nour K Majbour,Nishant N Vaikath,Simona S Ghanem,Indulekha P Sudhakaran,Houari Abdesselem,Agaristi Lamprokostopoulou,Kostas Vekrellis,Christopher M Morris,Johannes Attems,Omar M A El-Agnaf

View all publications

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Product promise

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Anti-Alpha-synuclein (phospho Y125) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein (phospho Y125) antibody (AB131466)

Western blot - Anti-Alpha-synuclein (phospho Y125) antibody (AB131466)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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