AB194775

Anti-Alpha-synuclein (phospho Y136) antibody

Name: Anti-Alpha-synuclein (phospho Y136) antibody (ab194775) | Abcam
Brand: Abcam Limited
SKU: AB194775
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Alpha-synuclein phospho Y136 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SNCA phospho Y136.

View Alternative Names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein (phospho Y136) antibody (AB194775)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein (phospho Y136) antibody (AB194775)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling alpha Synuclein (phospho Y136) with ab194775 at 1/100 dilution.

Supplier Data

Western blot - Anti-Alpha-synuclein (phospho Y136) antibody (AB194775)

All lanes:

Western blot - Anti-Alpha-synuclein (phospho Y136) antibody (ab194775) at 1/500 dilution

Lane 1:

Mouse brain tissue extract with blocking peptide

Lane 2:

Mouse brain tissue extract

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human SNCA phospho Y136. The exact immunogen used to generate this antibody is proprietary information.

P37840

Specificity

Due to high sequence homology ab194775 might react with Beta synuclein

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.05% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed : 20798282, PubMed : 26442590, PubMed : 28288128, PubMed : 30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed : 28288128, PubMed : 30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed : 30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed : 20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed : 26442590).

See full target information SNCA phospho Y136

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 20:20251206 PubMed40918165

2025

Peptide CCAT1-70aa promotes hepatocellular carcinoma proliferation and invasion via the MAPK/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xianjian Wu,Ruifeng Liang,Guoman Liu,Quan Fang,Zuoming Xu,Wenchuan Li,Chuan Tan,Jian Pu

NPJ Parkinson's disease 9:4 PubMed36646701

2023

Dynamic physiological α-synuclein S129 phosphorylation is driven by neuronal activity.

Applications

Unspecified application

Species

Unspecified reactive species

Nagendran Ramalingam,Shan-Xue Jin,Tim E Moors,Luis Fonseca-Ornelas,Kazuma Shimanaka,Shi Lei,Hugh P Cam,Aurelia Hays Watson,Lisa Brontesi,Lai Ding,Dinc Yasat Hacibaloglu,Haiyang Jiang,Se Joon Choi,Ellen Kanter,Lei Liu,Tim Bartels,Silke Nuber,David Sulzer,Eugene V Mosharov,Weisheng V Chen,Shaomin Li,Dennis J Selkoe,Ulf Dettmer

Acta neuropathologica communications 9:182 PubMed34772466

2021

Tyrosine 136 phosphorylation of α-synuclein aggregates in the Lewy body dementia brain: involvement of serine 129 phosphorylation by casein kinase 2.

Applications

Unspecified application

Species

Unspecified reactive species

Kazunori Sano,Yasushi Iwasaki,Yuta Yamashita,Keiichi Irie,Masato Hosokawa,Katsuya Satoh,Kenichi Mishima

View all publications

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Product promise

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Anti-Alpha-synuclein (phospho Y136) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein (phospho Y136) antibody (AB194775)

Western blot - Anti-Alpha-synuclein (phospho Y136) antibody (AB194775)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise