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Rabbit Recombinant Monoclonal Alpha-synuclein phospho Y39 antibody. Suitable for WB, IP, Dot and reacts with Transfected cell lysate - Mouse, Transfected cell lysate - Human, Synthetic peptide - Human samples.

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Images

Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (AB317366), expandable thumbnail
  • Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (AB317366), expandable thumbnail
  • Dot Blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (AB317366), expandable thumbnail
  • Immunoprecipitation - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (AB317366), expandable thumbnail
  • Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (AB317366), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPDotIHC-PICC/IF
Human
Predicted
Predicted
Predicted
Not recommended
Not recommended
Mouse
Predicted
Predicted
Predicted
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide - Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Mouse
Dilution info
1/1000
Notes

-

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/30
Notes

-

Predicted
Predicted

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Mouse, Synthetic peptide - Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide - Human
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Mouse, Transfected cell lysate - Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Mouse, Transfected cell lysate - Human, Synthetic peptide - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell lysate - Mouse, Transfected cell lysate - Human, Synthetic peptide - Human, Rat
Dilution info
-
Notes

-

Target data

Function

The protein expressed by the SNCA gene is involved in various synaptic activities, including the regulation of synaptic vesicle trafficking and neurotransmitter release. As a monomer, it enhances synaptic vesicle exocytosis through vesicle priming, fusion, and dilation of exocytotic fusion pores. Mechanistically, it increases local Ca(2+) release, which is crucial for ATP-induced exocytosis. In its multimeric membrane-bound form, SNCA acts as a molecular chaperone, assisting in the folding of synaptic fusion components known as SNAREs at the presynaptic plasma membrane, in conjunction with cysteine string protein-alpha/DNAJC5, a function that is vital for maintaining normal SNARE-complex assembly during aging. Additionally, SNCA plays a role in dopamine neurotransmission regulation by associating with the dopamine transporter (DAT1) and modulating its activity. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

Recommended products

Rabbit Recombinant Monoclonal Alpha-synuclein phospho Y39 antibody. Suitable for WB, IP, Dot and reacts with Transfected cell lysate - Mouse, Transfected cell lysate - Human, Synthetic peptide - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25118-5
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Associated diseases and disorders

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366), expandable thumbnail

    Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366)

    Alpha-synuclein (phospho Y39) Western blot staining using rabbit Anti-Alpha-synuclein (phospho Y39) antibody

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) (25kDa) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector treated with 1 mM pervanadate for 30 minutes whole cell lysate (untreated membrane) at 20 µg

    Lane 2: 293T transfected with human Tyrosine-protein kinase ABL1 expression vector and a mouse Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate (untreated membrane) at 20 µg

    Lane 3: 293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector treated with 1 mM pervanadate for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

    Lane 4: 293T transfected with human Tyrosine-protein kinase ABL1 expression vector and a mouse Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 25 kDa

    Exposure time: 6s

  • Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366), expandable thumbnail

    Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366)

    In Western blot, Anti-Alpha-synuclein antibody [EPR20535] (Anti-Alpha-synuclein antibody [EPR20535] ab212184) staining at 1/1000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with humanTyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein (WT) expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate at 4 µg with NFDM/TBST

    Lane 2: 293T transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein (mutant Y39) protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate at 4 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 25 kDa

    Exposure time: 10s

  • Dot Blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366), expandable thumbnail

    Dot Blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366)

    Dot blot analysis of Alpha-synuclein (phospho Y39) using ab317366 at 1:1000 (0.504 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    All lanes: Dot Blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366) at 1/1000 dilution

    Lane 1: Human Alpha-synuclein (phospho Y39) peptide a

    Lane 2: Human Alpha-synuclein (phospho Y39) peptide b

    Lanes 3 and 5: Human Alpha-synuclein non-phospho peptide

    Lane 4: Human Alpha-synuclein (phospho S131) peptide

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Immunoprecipitation - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366), expandable thumbnail

    Immunoprecipitation - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366)

    Alpha-synuclein (phospho Y39) was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate with ab317366 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317366 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate

    Lane 2: ab317366 IP in 293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab317366 in 293T transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate

    All lanes: Immunoprecipitation - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366) at 1/30 dilution

    All lanes: 293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate with NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 5s

  • Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366), expandable thumbnail

    Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366)

    In Western blot, Anti-Alpha-synuclein antibody [EPR20535] (Anti-Alpha-synuclein antibody [EPR20535] ab212184) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containi a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate (untreated membrane) at 4 µg with NFDM/TBST

    Lane 2: 293T transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containi a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 4 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 25 kDa

    Exposure time: 8s

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Product protocols

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