Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - BSA and Azide free (AB209348)

ab179484 staining alpha Tubulin in HFF-1 (Human skin fibroblast) cell line by ICC/IF (Immunocytochemistry/Immunofluorescence). The cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1 : 20000 dilution. An AlexaFluor^®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1 : 1000 dilution. An Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594), ab195889 was used as a counterstain at 1 : 200 dilution. DAPI was used as a nuclear counterstain. Confocal image showing cilia (arrows) staining in HFF-1 cells treated with starvation for 48 hours.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179484).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - BSA and Azide free (AB209348)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells treated with 500 ng/ml Trichostatin A for 4 hours labeling alpha Tubulin (acetyl K40) with ab179484 at 1/240 dilution (red line). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. ab179484 preincubated with 1mg/ml acetyl Alpha tubulin (acetyl K40) peptide (green) or non-acetyl Alpha tubulin (acetyl K40) peptide (orange). The isotype control was Rabbit monoclonal IgG (black) and the unlabelled contol was cells without incubation with primary antibody and secondary antibody (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179484).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - BSA and Azide free (AB209348)

ab179484 stained in Hela cells. Untreated and Trichostatin A treated (50ug/ml, 4 hours) cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab179484 at 1/500 dilution overnight at +4°C. The secondary antibody was ab150177 used at 1 ug/ml for 1hour at room temperature (colored green). DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179484).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - BSA and Azide free (AB209348)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling alpha Tubulin (acetyl K40) with ab179484 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining is observed on neuron cells of Human brain tissue. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179484).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - BSA and Azide free (AB209348)

Alpha Tubulin was immunoprecipitated from 1mg of HeLa cells (Human epithelial cells from cervix adenocarcinoma) treated with 500 ng/ml Trichostatin A for 4 hours with ab179484 at 1/70 dilution. Western blot was performed from 10 µg of the immunoprecipitate using ab179484 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane : Hela whole cell extract. Right lane : PBS instead of Hela whole cell extract.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179484).

All lanes:

Immunoprecipitation - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (<a href='/en-us/products/primary-antibodies/alpha-tubulin-acetyl-k40-antibody-epr16772-ab179484'>ab179484</a>)

Predicted band size: 50 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - BSA and Azide free (AB209348)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling alpha Tubulin (acetyl K40) with ab179484 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining is observed on neuron cells of Mouse cerebral cortex tissue. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179484).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - BSA and Azide free (AB209348)

This IHC data was generated using the same anti-alpha Tubulin (acetyl K40) antibody clone, EPR16772, in a different buffer formulation (cat# ab179484).

Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling alpha Tubulin (acetyl K40) with ab179484 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasmic staining is observed on Purkinje cells of cerebellum. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - BSA and Azide free (AB209348)

ab179484 staining alpha Tubulin in NIH/3T3 (mouse embryonic fibroblast) cell line by ICC/IF (Immunocytochemistry/Immunofluorescence). The cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1 : 20000 dilution. An AlexaFluor^®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1 : 1000 dilution. An Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594), ab195889 was used as a counterstain at 1 : 200 dilution. DAPI was used as a nuclear counterstain. Confocal image showing midbody (arrows) staining in NIH/3T3 cells treated with starvation for 48 hours.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179484).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] - BSA and Azide free (AB209348)

This ICC/IF data was generated using the same anti-alpha Tubulin (acetyl K40) antibody clone, EPR16772, in a different buffer formulation (cat# ab179484).

ab179484 staining alpha Tubulin in NIH/3T3 (mouse embryonic fibroblast) cell line by ICC/IF (Immunocytochemistry/Immunofluorescence).The cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1 : 20000 dilution. An AlexaFluor^®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1 : 1000 dilution. An Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594), ab195889 was used as a counterstain at 1 : 200 dilution. DAPI was used as a nuclear counterstain. Confocal image showing cilia (arrows) staining in NIH/3T3 cells treated with starvation for 48 hours.