AB11304

Anti-alpha Tubulin antibody [B-5-1-2] - Microtubule Marker

Name: Anti-alpha Tubulin antibody [B-5-1-2] - Microtubule Marker (ab11304) | Abcam
Brand: Abcam Limited
SKU: AB11304
Price: 420 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(61 Publications)

Mouse Monoclonal Alpha-tubulin 4 antibody. Suitable for ICC and reacts with Human samples. Cited in 61 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human TUBA4A.

View Alternative Names

TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain

2 Images

Supplier Data

Immunocytochemistry - Anti-alpha Tubulin antibody [B-5-1-2] - Microtubule Marker (AB11304)

ab11304 staining alpha Tubulin in the HEK293T cell line by ICC/IF (Immunocytochemistry/immunofluorescence) transfected with a recombinant protein HA tagged dual stained with Anti-HA Mouse primary antibody and Anti-Mouse Alexa Fluor®543 secondary antibody. Microtubules detected with Anti-α-tubulin Mouse primary antibody and Anti-Mouse Alexa Fluor®488 secondary antibody.Nuclus visualised with DAPI.

AbReview4521****

Immunocytochemistry - Anti-alpha Tubulin antibody [B-5-1-2] - Microtubule Marker (AB11304)

ab11304 at a 1/100 dilution staining human HeLa cells by Immunocytochemistry/Immunofluorescence. The cells were methanol fixed and blocked for 1 hour with 10% serum. The antibody was then incubated with the cells for 1 hour. Bound antibody was detected using a Goat anti-mouse TRITC antibody.

This image is courtesy of an Anonymous Abreview

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B-5-1-2

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC

applications

Immunogen

Native Full Length Protein corresponding to Human TUBA4A.

P68366

Epitope

The antibody recognizes an epitope located at the C-terminal end of the alpha tubulin isoform.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>" }, "African green monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Cynomolgus monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Xenopus laevis": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Xenopus tropicalis": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }

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Product details

Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.

Biological function summary

Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.

Pathways

Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.

Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBA4A

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Publications (61)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 10:503 PubMed39695179

2024

An HuR mutant, HuR-V225I, identified in adult T-cell Leukemia/Lymphoma, alters the pro-apoptotic function of HuR.

Applications

Unspecified application

Species

Unspecified reactive species

Bianca Colalillo,Sujitha Sali,Ali H Aldouhki,Isabelle Aubry,Sergio Di Marco,Michel L Tremblay,Imed E Gallouzi

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2406009 PubMed39018254

2024

CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Li,Qingzhou Chen,Tao Zheng,Fulin Wang,Junlin Teng,Haining Zhou,Jianguo Chen

Cell & bioscience 14:91 PubMed38997783

2024

SUMOylation of nuclear receptor Nor1/NR4A3 coordinates microtubule cytoskeletal dynamics and stability in neuronal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Gagnon,Véronique Caron,André Tremblay

Nature communications 15:5678 PubMed38971858

2024

Erythroid-intrinsic activation of TLR8 impairs erythropoiesis in inherited anemia.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Liang,Yang Wan,Jie Gao,Lingyue Zheng,Jingwei Wang,Peng Wu,Yue Li,Bingrui Wang,Ding Wang,Yige Ma,Biao Shen,Xue Lv,Di Wang,Na An,Xiaoli Ma,Guangfeng Geng,Jingyuan Tong,Jinhua Liu,Guo Chen,Meng Gao,Ryo Kurita,Yukio Nakamura,Ping Zhu,Hang Yin,Xiaofan Zhu,Lihong Shi

EMBO reports 24:e57300 PubMed37987447

2023

Microautophagy regulated by STK38 and GABARAPs is essential to repair lysosomes and prevent aging.

Applications

Unspecified application

Species

Unspecified reactive species

Monami Ogura,Tatsuya Kaminishi,Takayuki Shima,Miku Torigata,Nao Bekku,Keisuke Tabata,Satoshi Minami,Kohei Nishino,Akiko Nezu,Maho Hamasaki,Hidetaka Kosako,Tamotsu Yoshimori,Shuhei Nakamura

Scientific reports 13:17985 PubMed37863933

2023

The crosstalk between glomerular endothelial cells and podocytes controls their responses to metabolic stimuli in diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Albrecht,Carsten Sticht,Tabea Wagner,Steffen A Hettler,Carolina De La Torre,Jiedong Qiu,Norbert Gretz,Thomas Albrecht,Benito Yard,Jonathan P Sleeman,Boyan K Garvalov

Arthritis & rheumatology (Hoboken, N.J.) 75:517-532 PubMed36245290

2023

Reset of Inflammatory Priming of Joint Tissue and Reduction of the Severity of Arthritis Flares by Bromodomain Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Jasna Friščić,Christiane Reinwald,Martin Böttcher,Miranda Houtman,Maximilien Euler,Xi Chen,Kellie I Walker,Philipp Kirchner,Honglin Zhu,Benjamin Wirth,Daniela Weidner,René Krüger,Vladimir Trajkovic,Arif B Ekici,Kerstin Klein,Dimitrios Mougiakakos,Caroline Ospelt,Georg Schett,Markus H Hoffmann

Nature 611:167-172 PubMed36261522

2022

Substrate-driven assembly of a translocon for multipass membrane proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Arunkumar Sundaram,Melvin Yamsek,Frank Zhong,Yogesh Hooda,Ramanujan S Hegde,Robert J Keenan

Cells 11: PubMed36139370

2022

MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian Insufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Zobia Umair,Mi-Ock Baek,Jisue Song,Seona An,Seung Joo Chon,Mee-Sup Yoon

Cell reports 38:110444 PubMed35235784

2022

Age-associated decline of MondoA drives cellular senescence through impaired autophagy and mitochondrial homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hitomi Yamamoto-Imoto,Satoshi Minami,Tatsuya Shioda,Yurina Yamashita,Shinsuke Sakai,Shihomi Maeda,Takeshi Yamamoto,Shinya Oki,Mizuki Takashima,Tadashi Yamamuro,Kyosuke Yanagawa,Ryuya Edahiro,Miki Iwatani,Mizue So,Ayaka Tokumura,Toyofumi Abe,Ryoichi Imamura,Norio Nonomura,Yukinori Okada,Donald E Ayer,Hidesato Ogawa,Eiji Hara,Yoshitsugu Takabatake,Yoshitaka Isaka,Shuhei Nakamura,Tamotsu Yoshimori

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha Tubulin antibody [B-5-1-2] - Microtubule Marker

Immunocytochemistry - Anti-alpha Tubulin antibody [B-5-1-2] - Microtubule Marker (AB11304)

Immunocytochemistry - Anti-alpha Tubulin antibody [B-5-1-2] - Microtubule Marker (AB11304)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (61)

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