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Rabbit Monoclonal Alpha-tubulin 4 antibody. Microtubule marker. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Pig, Human, Drosophila melanogaster samples. Cited in 240 publications.
Alternative names=Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain, TUBA4A, TUBA1
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected | Expected |
| Rat | Tested | Tested | Expected | Expected |
| Drosophila melanogaster | Expected | Expected | Expected | Expected |
| Pig | Tested | Tested | Expected | Expected |
Images
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Pig | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Drosophila melanogaster | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/50000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/50000 | Notes - |
Species Pig | Dilution info 1/1000 - 1/50000 | Notes - |
Species Human | Dilution info 1/1000 - 1/50000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Drosophila melanogaster | Dilution info 1/1000 - 1/50000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Pig | Dilution info - | Notes - |
Species Drosophila melanogaster | Dilution info 1/250 - 1/500 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/50 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Pig | Dilution info - | Notes - |
Species Drosophila melanogaster | Dilution info 1/20 - 1/50 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target data
Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Recommended products
Rabbit Monoclonal Alpha-tubulin 4 antibody. Microtubule marker. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Pig, Human, Drosophila melanogaster samples. Cited in 240 publications.
Alternative names=Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain, TUBA4A, TUBA1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EP1332Y
- Purification technique
Affinity purification Protein A
- Specificity
This antibody is expected to recognise most alpha tubulin proteins and not only TUBA4A.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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