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Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560) is a rabbit monoclonal antibody detecting alpha Tubulin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human.



- Biophysical QC for unrivalled batch-batch consistency

- Over 40 publications



Images

Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (AB176560), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (AB176560), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (AB176560), expandable thumbnail
  • Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (AB176560), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (AB176560), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted
African green monkey
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/350
Notes

For unpurified, use 1/100.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat, African green monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000 - 1/10000
Notes

For unpurified, use 1/1000 - 1/10000.

Predicted
Predicted

Species
Mouse, Rat, African green monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/350
Notes

For unpurified, use 1/100.

Predicted
Predicted

Species
Mouse, Rat, African green monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/60
Notes

For unpurified, use 1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat, African green monkey
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Alternative names

TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain

Recommended products

Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560) is a rabbit monoclonal antibody detecting alpha Tubulin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human.



- Biophysical QC for unrivalled batch-batch consistency

- Over 40 publications


Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13478(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of alpha Tubulin?


Anti-alpha Tubulin [EPR13478(B)] - Loading Control (ab176560) is often used as a loading control in Western blot. It specifically detects a band for alpha Tubulin (UniProt: P68366) at a molecular weight of 50kDa.

Trusted by the scientific community


Anti-alpha Tubulin [EPR13478(B)] - Loading Control (ab176560) was first used in a scientific publication in 2013 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists


Anti-alpha Tubulin [EPR13478(B)] - Loading Control (ab176560) has over 10 independent reviews from customers.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products


We have a range of other formats of antibody clone [EPR13478(B)] also available for your convenience:
ab176560, HRP - HRP Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab185067, Alexa Fluor® 405 - Alexa Fluor® 405 Anti-alpha Tubulin antibody [EPR13478(B)] - Microtubule Marker ab210167, Carrier free - Anti-alpha Tubulin antibody [EPR13478(B)] - BSA and Azide free ab220805, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-alpha Tubulin antibody [EPR13478(B)] - Microtubule Marker ab225254, PE - PE Anti-alpha Tubulin antibody [EPR13478(B)] - Microtubule Marker ab225255, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-alpha Tubulin [EPR13478(B)] - Microtubule Marker ab305219, APC - APC Anti-alpha Tubulin antibody [EPR13478(B)] - Microtubule Marker ab314400



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.

Biological function summary

Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.

Pathways

Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.

Associated diseases and disorders

Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560), expandable thumbnail

    Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560) at 1/7000 dilution

    All lanes: Jurkat cell lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 50 kDa

    Observed band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue labeling alpha Tubulin with unpurified ab176560 at a 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Immunohistochemical staining of paraffin embedded human kidney with purified ab176560 at a dilution of 1/350. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560), expandable thumbnail

    Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    All lanes: Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Lane 3: A431 cell lysate at 10 µg

    Lane 4: K562 cell lysate at 10 µg

    Developed using the ECL technique.

    Predicted band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Immunohistochemical staining of paraffin embedded human kidney with unpurified ab176560 at a dilution of 1/100. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Intracellular Flow Cytometry analysis of permeabilized HeLa cells labeling alpha Tubulin (pink) with purifiedab176560 at a 1/70 dilution, or negative control rabbit IgG (green). The secondary antibody was FITC goat anti-rabbit.

  • Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Intracellular Flow Cytometry analysis of permeabilized Jurkat cells labeling alpha Tubulin (red) with unpurified ab176560 at a 1/10 dilution, or negative control rabbit IgG (green)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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