AB184970

Anti-alpha Tubulin antibody [EPR13799] - Loading Control

RabMAb
Recombinant
What is this?

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(31 Publications)

Rabbit Recombinant Monoclonal Alpha-tubulin 4 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 31 publications.

View Alternative Names

TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain

1 Images

Supplier Data

Western blot - Anti-alpha Tubulin antibody [EPR13799] - Loading Control (AB184970)

All lanes:

Western blot - Anti-alpha Tubulin antibody [EPR13799] - Loading Control (ab184970) at 1/50000 dilution

All lanes:

Saccharomyces cerevisiae extract at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13799

Isotype

IgG

Carrier free

Reacts with

Saccharomyces cerevisiae

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.

Biological function summary

Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.

Pathways

Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.

Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBA4A

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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1782 PubMed39972035

2025

Structural basis of urea transport by Arabidopsis thaliana DUR3.

Applications

Unspecified application

Species

Unspecified reactive species

Weidong An,Yiwei Gao,Laihua Liu,Qinru Bai,Jun Zhao,Yan Zhao,Xuejun C Zhang

Scientific reports 14:11497 PubMed38769106

2024

Upregulation of the AMPK-FOXO1-PDK4 pathway is a primary mechanism of pyruvate dehydrogenase activity reduction in tafazzin-deficient cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuqing Liang,Tyler Ralph-Epps,Michael W Schmidtke,Pablo Lazcano,Simone W Denis,Mária Balážová,Nevton Teixeira da Rosa,Mohamed Chakkour,Sanaa Hazime,Mindong Ren,Michael Schlame,Riekelt H Houtkooper,Miriam L Greenberg

Nature microbiology 9:1738-1751 PubMed38649409

2024

The nematode-trapping fungus Arthrobotrys oligospora detects prey pheromones via G protein-coupled receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Yen Kuo,Rebecca J Tay,Hung-Che Lin,Sheng-Chian Juan,Guillermo Vidal-Diez de Ulzurrun,Yu-Chu Chang,Jason Hoki,Frank C Schroeder,Yen-Ping Hsueh

Molecular cell 84:386-400.e11 PubMed38103558

2023

Ubiquiton-An inducible, linkage-specific polyubiquitylation tool.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Renz,Evrydiki Asimaki,Cindy Meister,Véronique Albanèse,Kirill Petriukov,Nils C Krapoth,Sabrina Wegmann,Hans-Peter Wollscheid,Ronald P Wong,Amitkumar Fulzele,Jia-Xuan Chen,Sébastien Léon,Helle D Ulrich

BMC complementary medicine and therapies 23:461 PubMed38102661

2023

Proteomic analysis reveals the molecular mechanism of Astragaloside in the treatment of non-small cell lung cancer by inducing apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqi Liu,Yan Sun,Wenjing Chen,Lingling Deng,Mengmeng Chen,Jingcheng Dong

Molecular cell 83:4461-4478.e13 PubMed38029752

2023

A direct interaction between CPF and RNA Pol II links RNA 3' end processing to transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Manuel Carminati,Juan B Rodríguez-Molina,M Cemre Manav,Dom Bellini,Lori A Passmore

Nature communications 14:4487 PubMed37495621

2023

Architecture and autoinhibitory mechanism of the plasma membrane Na/H antiporter SOS1 in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhang Wang,Chengcai Pan,Qihao Chen,Qing Xie,Yiwei Gao,Lingli He,Yue Li,Yanli Dong,Xingyu Jiang,Yan Zhao

iScience 26:106635 PubMed37138775

2023

Differential Hsp90-dependent gene expression is strain-specific and common among yeast strains.

Applications

Unspecified application

Species

Unspecified reactive species

Po-Hsiang Hung,Chia-Wei Liao,Fu-Hsuan Ko,Huai-Kuang Tsai,Jun-Yi Leu

EMBO reports 24:e56019 PubMed37009824

2023

Rapid evolutionary repair by secondary perturbation of a primary disrupted transcriptional network.

Applications

Unspecified application

Species

Unspecified reactive species

Po-Chen Hsu,Yu-Hsuan Cheng,Chia-Wei Liao,Richard Ron R Litan,Yu-Ting Jhou,Florica Jean Ganaden Opoc,Ahmed A A Amine,Jun-Yi Leu

PLoS genetics 18:e1010576 PubMed36574460

2022

Stress combined with loss of the Candida albicans SUMO protease Ulp2 triggers selection of aneuploidy via a two-step process.

Applications

Unspecified application

Species

Unspecified reactive species

Marzia Rizzo,Natthapon Soisangwan,Samuel Vega-Estevez,Robert Jordan Price,Chloe Uyl,Elise Iracane,Matt Shaw,Jan Soetaert,Anna Selmecki,Alessia Buscaino

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-alpha Tubulin antibody [EPR13799] - Loading Control

Western blot - Anti-alpha Tubulin antibody [EPR13799] - Loading Control (AB184970)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (31)

Product promise